We narrowed to 15,769 results for: LEA

1. pLsCas13a-gRNA-1

   Plasmid

   #164865

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   LsCas13a-gRNA-1
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBT374_pattB-pCA-GFP-pA-attBSwa

   Plasmid

   #52553

   Purpose

   for PhiC31 integrase-mediated cassette exchange

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attB (full length)
   GFP
   

   Expression

   Mammalian

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EGFP-bSV-1-830

   Plasmid

   #40963

   Depositor

   Elizabeth Luna

   Article

   Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

   Insert

   supervillin (SVIL Bovine)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   amino acids 1-830

   Available Since

   Dec. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

4. knob-s48i48

   Plasmid

   #68391

   Purpose

   Knob protein fused to elastin-like polypeptide (S48I48)

   Depositor

   Andrew MacKay

   Article

   Sun et al J Control Release. 2011 Oct 30;155(2):21

   Insert

   Knob-S48I48
   

   Tags

   Knob

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPS832

   Plasmid

   #1357

   Depositor

   Pamela Silver

   Article

   Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

   Insert

   GSP1 (GSP1 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pGEXndoE(E662Q)

   Plasmid

   #47716

   Purpose

   Expresses GST-EndoE(E662Q) from E. faecalis in E. coli

   Depositor

   Mattias Collin, Vincent Fischetti

   Article

   Collin et al J Biol Chem. 2004 May 21;279(21):22558-7

   Insert

   ndoE
   

   Tags

   GST-EndoE(E186Q) and Glutathione S-transferase

   Expression

   Bacterial

   Mutation

   Changed Glu-662 to Gln

   Promoter

   tac

   Available Since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS423-SgH

   Plasmid

   #163972

   Purpose

   Helper plasmid for cloning gRNAs under the control of the SNR52 promoter.

   Depositor

   Jiazhang Lian

   Article

   Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pc3XB-ZF5

   Plasmid

   #13104

   Depositor

   Keith Joung

   Article

   Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

   Insert

   ZF5
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Hy_pPepck1 eGFP SV40

   Plasmid

   #132644

   Purpose

   Expresses eGFP mRNA from the Drosophila Pepck1 promoter

   Depositor

   Jeremy Wilusz

   Article

   Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

   Insert

   eGFP
   

   Expression

   Insect

   Promoter

   Pepck1

   Available Since

   Oct. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR Bmi1-C

    Plasmid

    #30511

    Depositor

    John Gurdon

    Article

    Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

    Insert

    Bmi1 (Bmi1 Mouse)
    

    Use

    Gateway - donor vector

    Mutation

    NO STOP

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAVS1-NEO-CAG-BFP-pA-GFP-pA

    Plasmid

    #149344

    Purpose

    Vector for targeting the Replace reporter to the AAVS1 locus

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    BFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-HIRAmut-polyA

    Plasmid

    #59782

    Purpose

    This Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    HIRA
    

    Expression

    Mammalian

    Mutation

    Change from 'GATCGAAGTTTG' to 'GAc…

    Promoter

    CMV and T7

    Available Since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pc3XB-ZF10

    Plasmid

    #13109

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    ZF10
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTEI134

    Plasmid

    #126071

    Purpose

    Vector for expression of plastid targeted YFP1-10 (T203Y) in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Insert

    Chloroplastic FNR transit peptide
    

    Use

    Binary

    Tags

    YFP1-10

    Expression

    Plant

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEPOR0SP0007

    Plasmid

    #117534

    Purpose

    Level0 Golden Gate part: CDS, LbCas12a

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    LbCas12a (with stop codon)
    

    Use

    CRISPR

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBSKΔB-CBh-Cas9n-pA

    Plasmid

    #82617

    Purpose

    Expresses Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Insert

    SpCas9n
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pc3XB-ZF64

    Plasmid

    #13200

    Depositor

    Daniel Voytas

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    ZF64
    

    Expression

    Mammalian

    Available Since

    Oct. 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pc3XB-ZF63

    Plasmid

    #13199

    Depositor

    Daniel Voytas

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    ZF63
    

    Expression

    Mammalian

    Available Since

    Oct. 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Hy_pMtnA nLuc SV40

    Plasmid

    #132654

    Purpose

    Expresses nanoLuciferase (nLuc) mRNA from the Drosophila Metallothionein A promoter

    Depositor

    Jeremy Wilusz

    Article

    Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG

    Expression

    Insect

    Promoter

    MtnA

    Available Since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLY103

    Plasmid

    #130937

    Purpose

    Engineered sgRNA-LEA1 (2 BoxB aptamers) generator circuit including promoter Plux2

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEA1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Plux2

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge