We narrowed to 165,343 results for: addgene

1. pCW-GFP-CtIP

   Plasmid

   #71109

   Purpose

   mammalian expession of GFP-CtlP

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   CtlP
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Dec. 9, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pMH-HA/Joseph2-Multitagged Plasmid

   Plasmid

   #226726

   Purpose

   Validates the expression and detection of epitope-tagged proteins of interest in mammalian cells.

   Depositor

   Richard Cripps

   Article

   Robinson et al Biotechniques. 2024;76(7):299-309. doi:

   Insert

   pMH-HA/Joseph2
   

   Tags

   HA, Myc, GFP, Flag, V5, His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 24, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pHSB04X

   Plasmid

   #117258

   Purpose

   Genome editing for Lactobacillus brevis ATCC367

   Depositor

   Sheng Yang

   Article

   Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

   Insert

   Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
   

   Use

   Other

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLIK DN RhoA

   Plasmid

   #84646

   Purpose

   Lentiviral expression of doxycycline-inducible dominant negative RhoA and co-expression of Venus

   Depositor

   Sanjay Kumar

   Article

   MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

   Insert

   RhoA (RHOA Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Dominant Negative

   Available Since

   Dec. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pSLIK CA Rac1

   Plasmid

   #84605

   Purpose

   Lentiviral expression of doxycycline-inducible constitutively active Rac1 and co-expression of Venus

   Depositor

   Sanjay Kumar

   Article

   MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Constitutively Active, Q61L

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pSLIK_hygro_BirA

   Plasmid

   #136537

   Purpose

   Lentiviral expression vector for an inducible BirA

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   BirA
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Expression

   Mammalian

   Available Since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBLU-VIPR

   Plasmid

   #220498

   Purpose

   Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).

   Depositor

   Alexander Espinosa

   Article

   Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

   Type

   Empty backbone

   Use

   CRISPR; Transposon

   Expression

   Mammalian

   Available Since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. P2-ECFP-pA (Construct 6)

   Plasmid

   #55198

   Purpose

   Synthetic promoter responsive to gRNA2

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   mCerulean
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   Minimal Ade MLP

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-hisMBP-AsCpf1-EC

   Plasmid

   #79007

   Purpose

   Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

   Depositor

   Jin-Soo Kim

   Article

   Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

   Insert

   AsCpf1
   

   Tags

   6xHis-MBP-NLS and NLS-HA

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. HLA_DRA_01_01

    Plasmid

    #165012

    Purpose

    Expression of HLA-DRA*01:01 cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    HLA-DRA (HLA-DRA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO-shBRCA1 #1

    Plasmid

    #44594

    Depositor

    Eros Lazzerini Denchi

    Article

    Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

    Insert

    BRCA1 (Brca1 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

12. UPB-stomach (UPB1)

    Plasmid

    #180784

    Purpose

    Unique Projection Barcode (UPB1)-Stomach for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    humanized ChR2-H134R (partial)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO-shBRCA1 #2

    Plasmid

    #44595

    Depositor

    Eros Lazzerini Denchi

    Article

    Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

    Insert

    BRCA1 (Brca1 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pBabe-Dvl2-GFP

    Plasmid

    #109148

    Purpose

    Stable Dvl2 expression

    Depositor

    Mariann Bienz

    Article

    Gammons et al J Cell Sci. 2016 Oct 15;129(20):3892-390

    Insert

    Dvl2 (DVL2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. UPB-lung (UPB2)

    Plasmid

    #180788

    Purpose

    Unique Projection Barcode (UPB2)-Lung for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    humanized ChR2-H134R (partial)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. TTVTFLPTPSLILQT_epitope

    Plasmid

    #165021

    Purpose

    Expression of CMV UL44 (38-52) fused to CD74

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    CMV UL44 (38-52)
    

    Use

    Lentiviral

    Tags

    CD74 at N-term; cathepsin S cleavage site (GRWHTV…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. prDNA spacer-LacOx50-20xMS2-Hyg

    Plasmid

    #210219

    Purpose

    Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    50xLacO repeats derived from: PMID: 12864855.
    24xMS2 repeats derived from Addgene plasmid # 31865
    

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGDP3 apmA

    Plasmid

    #112881

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    apmA (apmA Staphylococcus aureus)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    Pbla

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRRL MND WASp

    Plasmid

    #36248

    Depositor

    David Rawlings

    Article

    Astrakhan et al Blood. 2012 Mar 19.

    Insert

    WASp (WAS Human)
    

    Use

    Lentiviral

    Promoter

    MND

    Available Since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMD.RVG.CVS24-B2c

    Plasmid

    #19713

    Depositor

    Manfred Schubert

    Article

    Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

    Insert

    Rabies virus CVS24-B2c glycoprotein
    

    Expression

    Mammalian

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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