We narrowed to 39,967 results for: lat

1. pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

   Plasmid

   #84239

   Purpose

   Expresses ABA-inducible VPR-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   ABI-tagBFP-Sp dCas9
   PYL1-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

   Expression

   Mammalian

   Promoter

   CAG and PGK

   Available Since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFPN1-gamma-tubulin

   Plasmid

   #87956

   Purpose

   Expresses human gamma-tubulin with a GFP-tag on the C-terminal of the protein

   Depositor

   Maria Alvarado-Kristensson, Ana Carrera

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   gamma-tubulin 1 (TUBG1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The protein is a sh-gamma-tubulin resistant gene …

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBactin-mNeonGreen-CI

   Plasmid

   #196850

   Purpose

   Expression of green fluorescent reporter mNeonGreen driven by the strong Beta-actin promoter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mNeonGreen
   

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PRPF4

   Plasmid

   #155850

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   PRPF4 (PRPF4 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ7558 pHR (hU6-crB2M-EFS-PuroR-WPRE)

   Plasmid

   #214881

   Purpose

   Lentiviral vector encoding RfxCas13d targeting B2M guide

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Insert

   hU6-crB2M-EFS-PuroR-WPRE
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Str-Ii_SBP-EGFP-Golgin84

   Plasmid

   #65303

   Purpose

   synchronize trafficking of Golgin-84 from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Golgin-84 (GOLGA5 Human)
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRX-IZU-LAMP1-mRuby3

   Plasmid

   #222586

   Purpose

   Stable expression of LAMP1-mRuby3 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Retroviral

   Tags

   mRuby3

   Expression

   Mammalian

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmRFP-N1 human cofilin S3E

   Plasmid

   #50858

   Purpose

   expresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3E (CFL1 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   S3E psuedo-phosporylated, non-activatable

   Promoter

   CMV

   Available Since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pLenti-DsRed_IRES_MAPT:EGFP

   Plasmid

   #92196

   Purpose

   Lentiviral overexpression vector to make stable bicistronic cell line for Tau screen

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   DsRed-IRES-MAPT-EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRXIB-mRuby3-LC3

    Plasmid

    #221738

    Purpose

    Stable expression of mRuby3-LC3 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    LC3B (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    mRuby3

    Expression

    Mammalian

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. ICAM2-bio-His

    Plasmid

    #51758

    Purpose

    Expresses full-length Intercellular adhesion molecule 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ICAM2 (ICAM2 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Jag1-HA pIRES

    Plasmid

    #17336

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

    Insert

    Jagged1 (Jag1 Rat)
    

    Tags

    HA X3

    Expression

    Mammalian

    Mutation

    A silent C->G mutation at 3237

    Available Since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIRESpuroN3xFLAG-His-CDK5RAP3

    Plasmid

    #204555

    Purpose

    Expresses 3xFLAG-His-tagged CDK5RAP3 in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

    Insert

    CDK5RAP3 (AMY2A Human)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-5X-1-LARP7

    Plasmid

    #113545

    Purpose

    Produces an N-terminal GST-tagged full length LARP7 protein.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    LARP7 (LARP7 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PTGS1-bio-His

    Plasmid

    #53414

    Purpose

    Expresses full-length Prostaglandin G/H synthase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PTGS1 (PTGS1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST PTBP1 isoform 4

    Plasmid

    #108594

    Purpose

    Recombinant GST tagged protein production

    Depositor

    Chris Smith

    Article

    Coelho et al EMBO J. 2015 Mar 4;34(5):653-68. doi: 10

    Insert

    PTBP1 isoform 4 (PTBP1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    isoform 4 (isoform a)

    Promoter

    tac

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-CLASP2 512-650

    Plasmid

    #24409

    Depositor

    Torsten Wittmann

    Article

    Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

    Insert

    CLASP2 (512-650) (CLASP2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CLASP2 deletion mutant with only MT plus end trac…

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRXIP-GFP-Spo20(PABD)

    Plasmid

    #221758

    Purpose

    Stable expression of GFP-Spo20(PABD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    Spo20 (SOS1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    tandem Spo20(PABD) (amino acids 422-551)

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCK341

    Plasmid

    #192640

    Purpose

    Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Inserts

    Sp.pCas9-dSpRY
    BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    SoxS has R93A and S101A mutations and dSpRY has s…

    Promoter

    BBa_J23107 and Sp.pCas9

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid

    #84261

    Purpose

    Expresses AND gate ABA/GA-inducible VPR-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9
    PYL1-VPR-p2a-GID1-ABI
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), ABI, GAI, GID1, HA tag, PYL1, p2a, …

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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