We narrowed to 13,571 results for: GAN

1. pET21-pelB-LaM-3

   Plasmid

   #172769

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-3
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC-BETA-At

   Plasmid

   #53288

   Purpose

   Contains crtE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and an lcyB cDNA of Arabidopsis thaliana. Use with pY2F to produce alpha-carotene in E. coli.

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

   Insert

   lcyB (LYC Mustard Weed)
   

   Use

   Low copy number bacterial cloning vector

   Promoter

   none

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. p1A

   Plasmid

   #162692

   Purpose

   Expresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   H. neapolitanus CbbLS and S. elongatus Prk
   

   Tags

   N terminal 6x His tag on PRK

   Expression

   Bacterial

   Promoter

   PLtet0-1 promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE60 R124K wgMDH

   Plasmid

   #204258

   Purpose

   Bacterial expression of R124K wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R124K

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE60 R124Q wgMDH

   Plasmid

   #204259

   Purpose

   Bacterial expression of R124Q wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R124Q

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE60 R130E wgMDH

   Plasmid

   #204267

   Purpose

   Bacterial expression of R130E wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R130E

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC-DELTA

   Plasmid

   #53273

   Purpose

   Contains crtE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, together with the lcyE gene of Arabidopsis thaliana and thereby produces delta-carotene in E. coli

   Depositor

   Francis X Cunningham Jr

   Article

   Sun et al J Biol Chem. 1996 Oct 4;271(40):24349-52

   Insert

   lcyE (LUT2 Mustard Weed)
   

   Use

   Low copy number bacterial cloning vector

   Promoter

   Trc

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJS183

   Plasmid

   #134142

   Purpose

   Bacterial expression of GFP-Ska1 and Ska2

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-GFP-human Ska1; human Ska2
   

   Tags

   GFP

   Expression

   Bacterial

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. SG3ΔENV RT D185A

   Plasmid

   #145782

   Purpose

   Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and D185A mutation in reverse transcriptase (RT D185A).

   Depositor

   Wesley Sundquist

   Article

   Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

   Insert

   Reverse Transcriptase
   

   Use

   Lentiviral and Retroviral

   Expression

   Mammalian

   Mutation

   Changed Reverse Transcriptase Aspartic Acid 185 t…

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-C1-hFGD5

    Plasmid

    #50760

    Purpose

    expression of EGFP-tagged human FGD5

    Depositor

    Naoki Mochizuki

    Article

    Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

    Insert

    FGD5 (FGD5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-PM-CaNAR2

    Plasmid

    #64730

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    Lyn-CaNAR2
    

    Tags

    Cerulean3, N-terminal targeting sequence from Lyn…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXs-Sig10-Fc-IG

    Plasmid

    #183722

    Purpose

    Expresses the fusion protein containing the extracellular part of human Siglec-10 and the Fc region of human IgG1, and GFP

    Depositor

    Takeshi Tsubata

    Article

    Alborzian Deh Sheikh et al J Autoimmun. 2021 Jan;116:102571. doi: 1

    Insert

    Siglec-10
    

    Use

    Retroviral

    Tags

    Fc of human IgG1

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

    Plasmid

    #202571

    Purpose

    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    LINE-1
    

    Tags

    GFP-AI retrotransposition reporter and HaloTag7 o…

    Expression

    Mammalian

    Mutation

    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lyn-mCherry-GAI(1-92)

    Plasmid

    #37315

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Tags

    Lyn and mCherry

    Expression

    Mammalian

    Mutation

    contains aa 1-92

    Promoter

    CMV

    Available Since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

15. MSCV eIF4E IRES GFP

    Plasmid

    #18761

    Depositor

    Scott Lowe

    Article

    Wendel et al Nature. 2004 Mar 18. 428(6980):332-7.

    Insert

    eIF4E (Eif4e Mouse)
    

    Use

    Retroviral

    Tags

    IRES GFP

    Expression

    Mammalian

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pTol2pA2-mnx1:MLS-KillerRed

    Plasmid

    #115515

    Purpose

    Plasmid driving expression of membrane bound KillerRed fluorophore in zebrafish motor neurons

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Promoter

    -3mnx1

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEP4-MR1

    Plasmid

    #178603

    Purpose

    Mammalian expression of myc-tagged MR1

    Depositor

    Erik Procko

    Article

    McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

    Insert

    MR1 (MR1 Human)
    

    Tags

    Extracellular c-myc epitope ta and Signal peptide…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. GluR2_adRNA(1,20,6)-ADAR2(E488Q)

    Plasmid

    #124621

    Purpose

    Expresses adRNA(1,20,6) targeting the RAB7A transcript along with the ADAR2(E488Q)

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    ADAR2(E488Q)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E488Q hyperactive mutant

    Promoter

    CMV

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2

    Plasmid

    #182348

    Purpose

    mScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEYFP-Sec24D

    Plasmid

    #66614

    Purpose

    YFP fusion of Sec24D

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

    Insert

    Sec24D (SEC24D Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

21. ZJOM20

    Plasmid

    #133644

    Purpose

    Integration of PEX1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast peroxisome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-PEX1-2GFP (PEX1 Budding Yeast)
    

    Tags

    EGFP

    Expression

    Yeast

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3-RAB-EKARev(T/A)

    Plasmid

    #118475

    Purpose

    Negative-control mutant for RAB-EKARev biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-EKARev(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pU6-SAG1-DHFR

    Plasmid

    #80322

    Purpose

    Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Insert

    SAG1 sgRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET21-pelB-LaM-8

    Plasmid

    #172772

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-8
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-ExRai-AktAR(T/A)

    Plasmid

    #118463

    Purpose

    Negative-control mutant for ExRai-AktAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AktAR(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCast

    Plasmid

    #200169

    Purpose

    E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

    Depositor

    Yong-Su Jin

    Article

    Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-CaMKIIa-Archer1-EGFP

    Plasmid

    #60423

    Purpose

    Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing Archer1-EGFP fusion. Archer1's red fluorescence monitors changes in membrane voltage.

    Depositor

    Viviana Gradinaru

    Article

    Flytzanis et al Nat Commun. 2014 Sep 15;5:4894. doi: 10.

    Insert

    Archer1-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p2T-CMV-HNHx-ABE8eHD-BlastR

    Plasmid

    #176478

    Purpose

    A-to-G base editor with alternative targeting scope

    Depositor

    Gerald Schwank

    Article

    Villiger et al Mol Ther Nucleic Acids. 2021 Aug 26;26:5

    Insert

    HNHx-ABE8eHD
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. hsp70:Arl13b-mKate2

    Plasmid

    #105964

    Purpose

    heat shock inducible transgenesis, primary cillium marker

    Depositor

    Caren Norden

    Article

    Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

    Insert

    Arl13b (Arl13b Mouse)
    

    Tags

    mKate2

    Expression

    Bacterial

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHH0103_PTPN22_Y-phosphatase

    Plasmid

    #110048

    Purpose

    Protein expression and purification of PTPN22_Y-phosphatase

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PTPN22_Y-phosphatase (PTPN22 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDONR221_SLC22A1

    Plasmid

    #132034

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

    Insert

    SLC22A1 (SLC22A1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pCSIIbsr-4082NES(ForAMPK)

    Plasmid

    #170310

    Purpose

    The lentiviral vector for AMPK FRET-biosensor

    Depositor

    Michiyuki Matsuda

    Article

    Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

    Insert

    4082NES (FRET biosensor for AMPK activity)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHH0103_NR2F2_Hormone-recep

    Plasmid

    #109826

    Purpose

    Protein expression and purification of NR2F2_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR2F2_Hormone-recep (NR2F2 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMRX-IPU-mTagBFP2-Sec61B

    Plasmid

    #228136

    Purpose

    Expresses mTagBFP2-Sec61B in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    Sec61B (SEC61B Human)
    

    Use

    Retroviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28a-His-MinD_MinE

    Plasmid

    #133621

    Purpose

    Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

    Depositor

    Petra Schwille

    Article

    Loose et al Science. 2008 May 9;320(5877):789-92. do

    Insert

    His-MinD_MinE (minD, minE E. coli)
    

    Expression

    Bacterial

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. STIMATE-mCherry

    Plasmid

    #70676

    Purpose

    Expresses STIMATE or TMEM110 in mammalian cells

    Depositor

    Yubin Zhou

    Article

    Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

    Insert

    STIMATE (TMEM110 Human)
    

    Tags

    mcherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pcDNA3-Mito-CaNAR2

    Plasmid

    #64731

    Purpose

    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNAR2
    

    Tags

    Cerulean3, N-terminal 30 amino acids from DAKAP1,…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCAG-ChRmine-mRuby2-ST

    Plasmid

    #227777

    Purpose

    Cation channelrhodopsin ChRmine fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChRmine-mRuby2-ST
    

    Expression

    Mammalian

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET21-pelB-LaG-30

    Plasmid

    #172754

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-30
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pClneoMyc mouse LNX2

    Plasmid

    #37010

    Depositor

    Yutaka Hata

    Article

    Kansaku et al Oncogene. 2006 Aug 24;25(37):5071-84. Ep

    Insert

    LNX2 (Lnx2 Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 23, 2012

    Availability

    Academic Institutions and Nonprofits only