We narrowed to 6,653 results for: SIM;

1. pY2

   Plasmid

   #218115

   Purpose

   Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGem-nHyPer2:sHyper2

   Plasmid

   #84737

   Purpose

   Simultaneous expresion of nuclear and stromal HyPer2 in plant cells

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Insert

   HyPer2 targeted to nucleus
   

   Use

   Tags

   NLS-SV40

   Expression

   Plant

   Mutation

   Promoter

   pFMV/p35S

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMA-SpCas9-g10

   Plasmid

   #80793

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMA-SpCas9-g9

   Plasmid

   #80792

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMA-SpCas9-g8

   Plasmid

   #80791

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDB364

   Plasmid

   #110275

   Purpose

   N-terminal 10-His DsbC TEV-peptide fusion

   Depositor

   Christopher Bahl

   Article

   Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   10-His, TEV cleavage peptide, and mature DsbC (i.…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAWH

   Plasmid

   #133894

   Purpose

   Expression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22H

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBKWH

   Plasmid

   #133898

   Purpose

   Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN_TTGmiRc2

   Plasmid

   #25753

   Purpose

   Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter with GFP coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDDGFP-Leu2d-ScVrg4

    Plasmid

    #102334

    Purpose

    Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

    Insert

    YGL225W (VRG4 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGAL4-DBD-MCS-long

    Plasmid

    #145246

    Purpose

    Backbone vector expressing GAL4 DNA-binding domain followed by MCS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVC-Ds-Pax3a_enh5-E1b:eGFP-Ds

    Plasmid

    #119059

    Purpose

    Fluorescent vector with zebrafish pax3a enhancer 5 (strong activity)

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    pax3a_enh5-E1b:eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E1b minimal promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFJ2359 - intron GG2 - 250bp

    Plasmid

    #159878

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    intron GG2 - 250bp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFJ2369 - intron GG1 - 250bp

    Plasmid

    #159877

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    intron GG1 - 250bp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FLAG-mTIP49a

    Plasmid

    #15357

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

    Insert

    TATA binding protein interacting protein 49 kDa (Ruvbl1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pCFJ1149 - intron 3 smu-2 PATCs

    Plasmid

    #161516

    Purpose

    PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron 3 from smu-2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYZ_3WJrep_N19_switch

    Plasmid

    #132723

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N19_switch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. MTK4_001

    Plasmid

    #123810

    Purpose

    Encodes Bovine Growth Hormone polyadenylation signal as a Type 4 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Bgh pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. MTK234_032

    Plasmid

    #123995

    Purpose

    Encodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA1 -hAAVS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. PARP15-c020

    Plasmid

    #173101

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP15 (PARP15 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    N78-S267, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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