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mCh-Mfn2

Plasmid

#141156

Purpose

Mammalian expression and labeling mitofusin 2

Depositor

Gia Voeltz

Publication

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Mitofusin 2 (MFN2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGL4.20-HIF1A prom

Plasmid

#40173

Depositor

Alex Minella

Publication

Sengupta et al Mol Cell Biol. 2011 Sep;31(18):3885-95.

Insert

HIF1A promoter fragment (HIF1A Human)

Expression

Mammalian

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PERK 2: PERK.K618A.9E10.pCDNA

Plasmid

#21815

Depositor

David Ron

Publication

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (K618A) (Eif2ak3 Mouse)

Tags

Myc (9E10)

Expression

Mammalian

Mutation

kinase dead K618A mutant

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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M7 pdnPKA-GFP

Plasmid

#16716

Depositor

Randall Moon

Publication

Ungar et al Dev Biol. 1996 Aug 25;178(1):186-91. doi

Insert

dnPKA (Prkar1a Mouse)

Use

Xenopus expression

Tags

GFP

Mutation

Dominant negative. Mutations at cAMP binding site…

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCMV-Myc CDC4 R465C

Plasmid

#16653

Depositor

Bert Vogelstein

Publication

Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

Insert

CDC4 R465C (FBXW7 Human)

Tags

myc

Expression

Mammalian

Mutation

R465C

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA3 Flag WDR5

Plasmid

#15552

Depositor

Kai Ge

Publication

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

Wdr5 (Wdr5 Mouse)

Tags

Flag

Expression

Mammalian

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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HA.PKD.K/W

Plasmid

#10809

Depositor

Alex Toker

Publication

Storz et al J Biol Chem. 2003 May 16. 278(20):17969-

Insert

PKD (PRKD1 Human)

Tags

HA and Myc (inserted between amino acids 33&3…

Expression

Mammalian

Mutation

Kinase dead mutant: K612W (Lys 612 to Trp) (bp183…

Promoter

CMV

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pGEX-human p53-(1-393)

Plasmid

#24860

Depositor

Cheryl Arrowsmith

Publication

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

GST

Expression

Bacterial

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHage2-EF1a-TERT-IRES-Puro

Plasmid

#182590

Purpose

expresses TERT in mammalian cells

Depositor

Agnel Sfeir

Publication

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

hTERT (TERT Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTP264

Plasmid

#149334

Purpose

Bacterial expression of Biotin ligase BirA

Depositor

Dirk Görlich

Publication

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Biotin ligase BirA

Tags

His14 and bdNEDD8

Expression

Bacterial

Available since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Jag1-HA pIRES

Plasmid

#17336

Depositor

Joan Conaway, Ronald Conaway

Publication

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Jagged1 (Jag1 Rat)

Tags

HA X3

Expression

Mammalian

Mutation

A silent C->G mutation at 3237

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pGEX-2T PTP-1B

Plasmid

#8602

Depositor

Ben Neel

Insert

PTP-1B (PTPN1 Human)

Tags

GST

Expression

Bacterial

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Ub-G76V-YFP

Plasmid

#11949

Depositor

Nico Dantuma

Publication

Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

Insert

Ub-G76V-YFP

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of YFP. Glycine 76 …

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CAG-Cas9-T2A-EGFP-ires-puro

Plasmid

#78311

Purpose

Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

Depositor

Timo Otonkoski

Publication

Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

Insert

WT SpCas9-T2A-EGFP-ires-puromycin resistance

Use

CRISPR

Tags

T2A-EGFP-ires-puro

Expression

Mammalian

Promoter

CAG

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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Cas9-NAT

Plasmid

#64329

Purpose

Cas9 expression cassette carried by a yeast single-copy episome plasmid

Depositor

Yong-Su Jin

Publication

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette

Use

CRISPR

Expression

Yeast

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pF145 pAcGFP1 SOD1G93A

Plasmid

#26406

Depositor

Elizabeth Fisher

Publication

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

G93A

Available since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcDNA3 HA Arf1 ActQ71L

Plasmid

#10832

Depositor

Thomas Roberts

Publication

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf1 Q71L (ARF1 Human)

Tags

HA

Expression

Mammalian

Mutation

Q71L. Defective in GTP hydrolysis. Constitutive…

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pRS3 (CK2alpha, CK2beta)

Plasmid

#27092

Depositor

David Litchfield

Publication

Turowec et al Methods Enzymol. 2010;484:471-93.

Inserts

CK2beta (CSNK2B Human)
CK2alpha (CSNK2A1 Human)

Use

Tetracycline-regulated expression

Tags

HA and Myc

Expression

Mammalian

Promoter

bidirectional tet-responsive promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smurf2 C716A

Plasmid

#11747

Depositor

Jeff Wrana

Publication

Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

Insert

SMURF2 (SMURF2 Human)

Tags

Flag

Expression

Mammalian

Mutation

C716A: catalytically inactive

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

5 Articles

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single polypeptide FPX biosensor for caspase-3

Plasmid

#60883

Purpose

FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).

Depositor

Robert Campbell

Publication

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

single polypeptide FPX biosensor for caspase-3

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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