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pX330A_dCas9-1x5

Plasmid

#63599

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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hP14H8y

Plasmid

#84368

Purpose

antigen for human PRDM14 purification ( aa 1-243)

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14 (PRDM14 Human)

Tags

6xHis

Expression

Bacterial

Mutation

contains PRDM14 AA1-243

Promoter

T7

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-fishRab8A[T22N]

Plasmid

#24906

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8A

Use

Gateway entry vector

Mutation

Changed Threonine 22 to Asparagine

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
pRS406 ATG9-GFP S122D

Plasmid

#114667

Purpose

Expresses yeast ATG9 (S122D) under the endogenous promoter with a GFP C-terminal tag

Depositor

Daniel Klionsky

Article

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Mutation

changed serine 122 to aspartic acid

Promoter

ATG9

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3_ctrl_CP-candidate_luc+

Plasmid

#86392

Purpose

Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates basal activity (no enhancer in vector) is reflected by luciferase activity

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Type

Empty backbone

Use

Luciferase

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pUT1858-HQ858878

Plasmid

#27960

Depositor

John Gray, Erich Grotewold

Article

Yilmaz et al Plant Physiol. 2009 Jan;149(1):171-80. d

Insert

bHLH transcription factor encoded by Os01g0928000 LOC_Os01g70310

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch12_62418577-gRNA-for

Plasmid

#81216

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch12 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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palphaGGFPR

Plasmid

#64377

Purpose

Alpha-gliadin gene promoter driving GFP expression

Depositor

Claudia Vickers

Article

Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

Insert

alpha-Gliadin promoter

Tags

GFP

Expression

Plant

Promoter

alpha-Gliadin

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only
pOAGFPR

Plasmid

#64397

Purpose

Oat avenin promoter driving GFP expression

Depositor

Claudia Vickers

Article

Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

Insert

Oat avenin promoter

Tags

GFP

Expression

Plant

Promoter

Oat avenin

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
L1-YA

Plasmid

#15525

Depositor

Vance Lemmon

Article

Cheng et al J Neurochem. 2005 Aug . 94(4):1102-10.

Insert

L1-cytoplasmic domain-Y1176A (L1CAM Human)

Expression

Yeast

Mutation

Tyrosine 1176 changed to Alanine

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pFM111

Plasmid

#40157

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-1N:FLAG (par-1 Nematode)

Tags

Flag and GST

Expression

Bacterial

Mutation

Contains aa 1-964

Promoter

tac

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pX330A_dCas9-1x3

Plasmid

#63597

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBluescript-KS-Nrg3-ISH

Plasmid

#87669

Purpose

In situ probe for mouse Nrg3

Depositor

Oscar Marin

Article

Bartolini et al Cell Rep. 2017 Jan 31;18(5):1157-1170. d

Insert

Nrg3 (Nrg3 Mouse)

Expression

Bacterial

Promoter

T7/M13

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pRmHa3-dKDM4A-HA2FL2

Plasmid

#20232

Depositor

Jerry Workman

Article

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4A (Kdm4A Fly)

Tags

FLAG2 and HA2

Expression

Insect

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 N33-170C

Plasmid

#17526

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 34-159 (paired domain)

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 N215-273C

Plasmid

#17527

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 216-272 (homeodomain)

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pUT1613-HQ858850

Plasmid

#27932

Depositor

John Gray, Erich Grotewold

Article

Yilmaz et al Plant Physiol. 2009 Jan;149(1):171-80. d

Insert

bHLH transcription factor encoded by Os02g0433600 LOC_Os02g23823

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2-HA-Kdrl-3p-FokI-KK

Plasmid

#35685

Depositor

Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

Kdrl-3p-FokI-KK

Use

Mrna production

Tags

HA

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only
pCS2-Flag-Kdrl-5p-FokI-EL

Plasmid

#35684

Depositor

Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

Kdrl-5p-FokI-EL

Use

Mrna production

Tags

FLAG and Flag

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only
V677 pDNR MCS BE lacZ alpha - Donor

Plasmid

#11694

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Donor vector

Available Since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pBabe-Puro-RalA-S194A

Plasmid

#15255

Depositor

William Hahn

Article

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

v-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

serine 194 to alanine

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pENTR-fishRab8A[Q67L]

Plasmid

#24905

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8A

Use

Gateway entry vector

Mutation

Changed Glutamine 67 to Leucine

Available Since

June 28, 2010

Availability

Academic Institutions and Nonprofits only
pDZ529 pTRP MET25p PP7-PS-2x-yeGFP

Plasmid

#72233

Purpose

Tryptophan selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Expression

Yeast

Promoter

MET25

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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PcDNA3 - FmnI Iso1b

Plasmid

#19323

Depositor

Philip Leder

Article

Zhou et al Exp Cell Res. 2006 Apr 15. 312(7):1119-2

Insert

FmnI Iso1b (Fmn1 Mouse)

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 FL C214S

Plasmid

#47086

Depositor

Marina Ramirez-Alvarado

Article

Levinson et al Sci Rep. 2013;3:1278. doi: 10.1038/srep0

Insert

AL-09 FL C214S (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

C214S

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pME-basGal80

Plasmid

#51621

Purpose

vertebrate expression of Gal80 preceded by a basal promoter

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

Gal80 (GAL80 Budding Yeast)

Use

Vertebrate expression

Promoter

basal promoter

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-HPV16-E1(partial)

Plasmid

#53699

Purpose

Luciferase reporter assay for microRNA binding on HPV16 E1

Depositor

Edward Chan

Article

Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

Insert

HPV16-E1(partial)

Use

Luciferase

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only
pLL3.7 Alk.1 shRNA

Plasmid

#59296

Purpose

shRNA to mouse Alk

Depositor

Ulrike Heberlein

Article

Lasek et al J Neurosci. 2011 Oct 5;31(40):14134-41.

Insert

Alk.1 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pFM115

Plasmid

#40161

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3C (par-3 Nematode)

Tags

GST

Expression

Bacterial

Mutation

C terminal portion of protein (aa850-1286)

Promoter

tac

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
PCx35-pcDNA-S276A

Plasmid

#42924

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35

Expression

Mammalian

Mutation

Ser276 to Ala

Promoter

CMV

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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