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pBAD_LcgcC-GFP-H6

Plasmid

#245528

Purpose

His-tagged superfolder GFP C-terminally fused to C-terminal LCGC14 split intein fragment

Depositor

Henning Mootz

Article

Humberg et al Angew Chem Int Ed Engl. 2025 Oct 23:e202

Insert

C-terminal Fragment of the LCGC14 PolB2 Split Intein

Tags

Hexahistidine Tag and Superfolder Green Fluoresce…

Expression

Bacterial

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CrPV-IRES-cytERM-SUNTAG-MS2

Plasmid

#247299

Purpose

Mammalian expression of ER-specific SUNTAG reporter: piggyBac vector expressing cytERM-SUNTAG MS2; 5’ UTR includes cricket paralysis virus IRES, allowing escape from translation initiation regulation.

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

PB-CrPV-IRES-cytERM-SUNTAG-MS2

Expression

Mammalian

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1-FRT-HaloTag-TAF1A

Plasmid

#247347

Purpose

Expresses HaloTag-TAF1A

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

TAF1A (TAF1A) (TAF1A Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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huCof WT-mRFP

Plasmid

#245973

Purpose

Neuronal specific expression of cof-mRFP

Depositor

James Bamburg

Article

Flynn et al Dev Neurobiol. 2009 Oct;69(12):761-79. d

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

NSE

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-Lck-mCherry-ibARK

Plasmid

#241241

Purpose

Expression of plasma membrane-tethered ibARK in astrocytes

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

Lck-mCherry-ibARK

Use

AAV

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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hSyn1-mCherry-CAAX

Plasmid

#223674

Purpose

AAV expression of mCherry from hSyn1 promoter

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mCherry-CAAX

Use

AAV

Promoter

hSyn1

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CASTOR1-HA (D304A)

Plasmid

#237419

Purpose

Transient expression of C-terminally HA-tagged human CASTOR1 (D304A mutant)

Depositor

David Sabatini

Article

Valenstein et al Nature. 2025 Aug 20. doi: 10.1038/s41586

Insert

CASTOR1 (GATSL3 Human)

Tags

HA

Expression

Mammalian

Mutation

D304A

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pX458-SLC35A1

Plasmid

#239952

Purpose

Encodes gRNA for human SLC35A1

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

SLC35A1

Use

CRISPR

Expression

Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pX458-MFSD6

Plasmid

#239951

Purpose

Encodes gRNA for human MFSD6

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

MFSD6

Use

CRISPR

Expression

Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-ICAM-5-Puro

Plasmid

#239949

Purpose

CRISPR KO of human ICAM-5

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

ICAM-5

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-nb-KS

Plasmid

#238266

Purpose

For overexpression of V5-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-nb-KS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-MFSD6-Puro

Plasmid

#239950

Purpose

CRISPR KO of human MFSD6

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

MFSD6

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-nb-noKS

Plasmid

#238265

Purpose

For overexpression of V5-nb-noKS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-nb-noKS

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_mCherry-P2A

Plasmid

#238238

Purpose

For integration of mCherry control

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-P2A

Plasmid

#238231

Purpose

For overexpression of mCherry control

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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Nanog-mNG-KS

Plasmid

#238298

Purpose

For RNA injection of Nanog-mNG-KS into Zebrafish

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

Nanog-mNG-KS

Use

Zebrafish expression

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-EGFP-NPM1

Plasmid

#238264

Purpose

For overexpression of V5-EGFP-NPM1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-EGFP-NPM1

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-SURF6

Plasmid

#238252

Purpose

For overexpression of mCherry-SURF6

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-SURF6

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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Nanog-mNG

Plasmid

#238296

Purpose

For RNA injection of Nanog-mNG into Zebrafish

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

Nanog-mNG

Use

Zebrafish expression

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-nb-KS-FtoG

Plasmid

#238267

Purpose

For overexpression of V5-nb-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-nb-KS-FtoG

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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