We narrowed to 11,731 results for: nsf

1. GST-BHMT-I/G

   Plasmid

   #104442

   Purpose

   Autophagy cargo assay

   Depositor

   Carol Mercer

   Article

   Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

   Insert

   BHMT (BHMT Human)
   

   Tags

   GFP-myc GFP is from Aequorea victoria and GST

   Expression

   Mammalian

   Mutation

   N43A in GST (in a potential KFERQ-like sequence).…

   Promoter

   CMV

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. IL1RN gRNA3

   Plasmid

   #113132

   Purpose

   Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   IL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. IL1RN gRNA4

   Plasmid

   #113133

   Purpose

   Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   IL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, Synthetic Biology, and TALEN

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. IL1RN gRNA2

   Plasmid

   #113131

   Purpose

   Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. YPET-GGA3

   Plasmid

   #18841

   Depositor

   Martin Schwartz

   Article

   Hall et al Anal Biochem. 2008 Mar 15. 374(2):243-9.

   Insert

   YPET-GGA3 (GGA3 Human)
   

   Tags

   His6 and myc

   Expression

   Mammalian

   Mutation

   The fluorescent protein YPET was added to the ami…

   Available Since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pCMV5-Flag-EPLIN beta

   Plasmid

   #40929

   Depositor

   Elizabeth Luna, Scott Maul

   Article

   Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

   Insert

   EPLIN beta (LIMA1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Dec. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMCL-HA-MAPKK1-11A55B [S218E/S222D]

   Plasmid

   #40809

   Depositor

   Natalie Ahn

   Article

   Mansour et al Science. 1994 Aug 12;265(5174):966-70.

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S218E, S222D

   Promoter

   CMV

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pRSFDuet-gst-xr

   Plasmid

   #214761

   Purpose

   Xylose reductase from Hypocrea jecorina fused with GST-tagged and codon optimized for expression in E. coli

   Depositor

   Pimchai Chaiyen

   Article

   Jaroensuk et al J Biol Chem. 2023 Dec 28:105598. doi: 10

   Insert

   glutathione S-transferases fused with xylose reductase
   

   Tags

   Glutathione S-transferases

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. TPST2-EGFP

   Plasmid

   #66618

   Purpose

   Encodes GFP fusion of TPST2 trans Golgi marker (Tyrosyl protein sulfotransferase 2)

   Depositor

   David Stephens

   Article

   Spooner et al Biochem J. 2008 Sep 15;414(3):471-84. do

   Insert

   TPST2 (TPST2 Human)
   

   Expression

   Mammalian

   Mutation

   Insert contains T370A relative to NM_001008566.1

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCI-neo-Flag-DHHC5

    Plasmid

    #85812

    Purpose

    To rescue the expression of DHHC5 in the knockdown cell lines

    Depositor

    Sandra Hofmann

    Article

    Tian et al Mol Cancer Res. 2015 Apr;13(4):784-94. d

    Insert

    ZDHHC5 (Zdhhc5 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 deltaN47 Beta-catenin

    Plasmid

    #19287

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N-terminal truncation, deleted aa 1-47

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. KRas FAK biosensor

    Plasmid

    #78301

    Purpose

    FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    KRas FAK biosensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-T7-AcrIIC3-NLS(SV40) (KAC209)

    Plasmid

    #134341

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIC3 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIC3
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXJ-Myc-KLF2

    Plasmid

    #232654

    Purpose

    transient expresses KLF2 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    KLF2 (KLF2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pF420

    Plasmid

    #123659

    Purpose

    Produces cofactor F420 in E. coli

    Depositor

    Colin Jackson

    Article

    Bashiri et al Nat Commun. 2019 Apr 5;10(1):1558. doi:

    Inserts

    CofD
    FbiB
    FbiD
    FbiC
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BBa_R0040

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs-hu-N-Myc

    Plasmid

    #50772

    Purpose

    retroviral expression of human N-Myc

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    N-myc (MYCN Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV5-Flag-EPLIN alpha

    Plasmid

    #40928

    Depositor

    Elizabeth Luna, Scott Maul

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN alpha (LIMA1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Dec. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pRARE-MBP-DEST

    Plasmid

    #84650

    Purpose

    Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

    Depositor

    Neil Olszewski

    Article

    Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

    Type

    Empty backbone

    Tags

    MBP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5FRT/Flag-FLJ20436

    Plasmid

    #29757

    Depositor

    Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    FLJ20436 (C12orf41 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pRK5 TGF beta type I receptor (T202D) Flag

    Plasmid

    #14833

    Depositor

    Rik Derynck

    Article

    Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

    Insert

    TGF beta type I receptor (Tgfbr1 Rat)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Threonine 202 mutated to aspartate. Constitutivel…

    Available Since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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