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msHook2 g1 lentiCRISPRv2-mCherry

Plasmid

#218652

Purpose

Knockout vector for mouse Hook2

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Hook2 (Hook2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3-N-GFP

Plasmid

#222602

Purpose

Expression of MAC3-tagged GFP (N-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-C-GFP

Plasmid

#222601

Purpose

Expression of MAC3-tagged GFP (C-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pYPQ-Un1Cas12f1

Plasmid

#205143

Purpose

Un1Cas12f1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Tang et al Plant Genome. 2024 May 28:e20465. doi: 1

Insert

Un1Cas12f1

Use

CRISPR

Tags

NLS

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP)

Plasmid

#218640

Purpose

Gateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Mutation

delPYD (I125M-END)

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 hNLRP3 R262W (disease-associated mutation)

Plasmid

#218647

Purpose

Gateway shuttling vector for cloning NLRP3-R262W (no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (NLRP3 Human)

Use

Gateway entry

Mutation

R262W

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3(ΔPYD, I125M-END)

Plasmid

#218641

Purpose

Gateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Mutation

delPYD (I125M-END)

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3 (STOP)

Plasmid

#218638

Purpose

Gateway shuttling vector for cloning N-terminal NLRP3 tags (contains a stop codon--note: bulky N-terminal tags are likely not functional)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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msHook2 g2 lentiCRISPRv2-mCherry

Plasmid

#218653

Purpose

Knockout vector for mouse Hook2

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Hook2 (Hook2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pH2B-6AA-ffDronpa

Plasmid

#214606

Purpose

Expresses reversibly switchable fluorescent protein ffDronpa targeted to the nucleus

Depositor

Peter Dedecker

Article

Valenta et al Talanta. 2024 Mar

Insert

ffDronpa-H2B

Tags

H2B targetting with a linker of 6AA

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pffDronpa2F-peroxisome

Plasmid

#214608

Purpose

Expresses reversibly switchable fluorescent protein ffDronpa2F targeted to peroxisome

Depositor

Peter Dedecker

Article

Valenta et al Talanta. 2024 Mar

Insert

ffDronpa2F-peroxisome

Tags

peroxisome targeting sequence (PTS)

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB12717

Plasmid

#212691

Purpose

integration vector for three gene cassette with integration site C3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Expression

Yeast

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB13089

Plasmid

#212676

Purpose

TEF promoter

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

TEF

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB12706

Plasmid

#212677

Purpose

EXP promoter

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

EXP

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB12708

Plasmid

#212678

Purpose

GPD promoter

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

GPD

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB13090

Plasmid

#212679

Purpose

Lip2 terminator

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

Lip2

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB12710

Plasmid

#212680

Purpose

Syn8 terminator

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

Syn8

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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PCfB12709

Plasmid

#212681

Purpose

SynGuo terminator

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

SynGuo

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB13014

Plasmid

#212685

Purpose

integration vector for single cassette with integration site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB12718

Plasmid

#212692

Purpose

integration vector for three gene cassette with integration site F3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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