19,635 results

1. METTL3 shRNA 1 tet pLKO puro

   Plasmid

   #162985

   Purpose

   Tet-inducible shRNA targeting human METTL3 #1

   Depositor

   Mark Rubin

   Article

   Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Use

   Lentiviral

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. hFerritinH

   Plasmid

   #122652

   Purpose

   Expression of hFerritinH in bacterial cells

   Depositor

   Trevor Douglas

   Insert

   ferritin heavy chain 1 (FTH1 Human)
   

   Expression

   Bacterial

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. ATF4 3: Mouse ATF4 (CHOP11/cATF), 5'UTR and AUG-luc

   Plasmid

   #21850

   Depositor

   David Ron

   Article

   Harding et al Mol Cell. 2000 Nov . 6(5):1099-108.

   Insert

   ATF4 (Atf4 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   ATF4 5' UTR and AUG fused to Luciferase driv…

   Available Since

   Oct. 8, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-DsRed2

   Plasmid

   #15777

   Depositor

   Edward Callaway

   Article

   Wickersham et al Neuron. 2007 Mar 1. 53(5):639-47.

   Insert

   DsRed2
   

   Expression

   Mammalian

   Available Since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pyEvolvR (enCas9-PolI5M)

   Plasmid

   #158073

   Purpose

   Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   John Dueber

   Article

   Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

   Insert

   enCas9-PolI5M
   

   Expression

   Bacterial and Yeast

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

   Plasmid

   #85006

   Purpose

   For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   EGFP-ires-tTA
   

   Expression

   Mammalian

   Available Since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-rab7 WT

   Plasmid

   #12605

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB7 (Homo sapiens) (RAB7A Human)
   

   Tags

   GFP and HA

   Expression

   Mammalian

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMK346 (Hygro-P2A-mAID-mCherry)

   Plasmid

   #121180

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Hygro-P2A-mAID-mCherry
   

   Use

   CRISPR

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_MYC_WT

   Plasmid

   #82927

   Purpose

   Gateway Donor vector containing MYC, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   Myc (MYC Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. IKK-2 S177E S181E

    Plasmid

    #11105

    Purpose

    Mammalian expression of constitutively active human IKK-2

    Depositor

    Anjana Rao

    Article

    Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

    Insert

    IKK-2 (IKBKB Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S177E and S181E, constitutively active

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

    Plasmid

    #67977

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmScarlet-i_C1

    Plasmid

    #85044

    Purpose

    Mammalian expression of mScarlet-i

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    mScarlet-i
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV6-Neo-ISG15

    Plasmid

    #80404

    Purpose

    overexpression of ISG15

    Depositor

    Isidoro Martinez

    Article

    Gonzalez-Sanz et al J Virol. 2016 Jan 13;90(7):3428-38. doi:

    Insert

    ISG15 (ISG15 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP(C3)-Nup153

    Plasmid

    #64268

    Purpose

    GFP-tagged mammalian expression of Nup153

    Depositor

    Birthe Fahrenkrog

    Article

    Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

    Insert

    NuP153 (NUP153 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3-EF1a-NICD1

    Plasmid

    #46047

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain initiating at amino acid 1753

    Promoter

    EF1a

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST-ORF-V2

    Plasmid

    #73638

    Purpose

    Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Tags

    Venus fragment 2 (V2)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAcGHLT-B-DDB1

    Plasmid

    #48638

    Purpose

    Expresses Full Lenght DDB1 in Insect Cells (ampR)

    Depositor

    Ning Zheng

    Article

    Li et al Cell. 2006 Jan 13;124(1):105-17.

    Insert

    DDB1 (DDB1 Human)
    

    Expression

    Insect

    Available Since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

18. sfGFP-Caveolin-C-10

    Plasmid

    #56366

    Purpose

    Localization: Membrane, Excitation: 485, Emission: 507

    Depositor

    Michael Davidson

    Insert

    Caveolin (CAV1 Human)
    

    Tags

    sfGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pCMV5-Flag-RhoA wt

    Plasmid

    #11750

    Depositor

    Jeff Wrana

    Article

    Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

    Insert

    RhoA (RHOA Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pRK5-LAMP1-FLAG

    Plasmid

    #71868

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    LAMP1 (LAMP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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