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VPS13C^Halo-△(ATG2C-PH)

Plasmid

#232867

Purpose

Expresses VPS13C-△(ATG2C-PH) in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

VPS13C-△(ATG2C-PH) (VPS13C Human)

Tags

internal Halo

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-VAB(VPS13C)

Plasmid

#232865

Purpose

Expresses the VAB domain of VPS13C in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

the VAB domain of VPS13C (VPS13C Human)

Tags

mCherry

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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1GFP/RNase H1 D210N

Plasmid

#174448

Purpose

Express GFP-tagged RNase H1 D210N in E. coli.

Depositor

Karlene Cimprich

Article

Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

RNase H1 (RNASEH1 Human)

Tags

His-tag, GFP

Expression

Bacterial

Mutation

D210N

Promoter

T7 promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-ATF4 mScarlet NLS

Plasmid

#115969

Purpose

ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

Depositor

David Andrews

Article

Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

Insert

activating transcription factor 4 (ATF4 Synthetic, Human)

Use

Lentiviral

Tags

HA tag, c-myc NLS, and mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-puro/Flag PD-L1 WT

Plasmid

#121446

Purpose

expression of huamn PD-L1 wildtype in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

wildtype

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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ODD-Luciferase-pcDNA3

Plasmid

#18965

Depositor

William Kaelin

Article

Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

Insert

ODD-Firefly Luciferase (HIF1A Human)

Expression

Mammalian

Mutation

HIF1a ODD sequence fused in frame to luciferase O…

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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GFP-LRRK2

Plasmid

#232872

Purpose

Expresses LRRK2 in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

GFP-LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_R-PTEN

Plasmid

#227433

Purpose

Expresses the R-PTEN sensor under a CMV promoter

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

R-PTEN (Pten Rat)

Tags

mCyRFP2, mMaroon

Expression

Mammalian

Mutation

R14G

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pinducer 20 DN-KASH

Plasmid

#125554

Purpose

Tet-ON inducible lentivirus expressing mcherry-labeled DN KASH

Depositor

Daniel Conway

Article

Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

Insert

SYNE1 (SYNE1 Human)

Use

Lentiviral

Tags

mcherry

Mutation

Truncated form: only the KASH domain of nesprin-…

Promoter

tetracycline responsive element

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pNK2657

Plasmid

#219741

Purpose

MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

hispidin- synthase from Mycena citricolor

Use

Synthetic Biology

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-SOD2-2A-Catalase-WPRE

Plasmid

#67635

Purpose

AAV vector expressing both SOD2 and mitochondrial targeted Catalase

Depositor

Connie Cepko

Article

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

SOD-2A-Catalase (Sod2, Cat Mouse)

Use

AAV

Mutation

Deleted the Peroxisome Targeting Signal from Cata…

Promoter

CMV

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-BRD3

Plasmid

#65377

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD3 (BRD3 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-BRD7

Plasmid

#65379

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD7 (BRD7 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-BRD9

Plasmid

#65380

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD9 (BRD9 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-FBXL11

Plasmid

#126543

Purpose

Expresses GFP-tagged FBXL11 in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

FBXL11 (KDM2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

LTR

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCF140_Gag-SpyCas9-NLS

Plasmid

#225959

Purpose

CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

Depositor

Christof Fellmann

Article

Ramadoss et al Nat Commun. 2025 Nov 17;16(1):9883. doi:

Insert

CMV-Intron-Gag-SpyCas9-NLS

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-BRDT

Plasmid

#65381

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRDT (BRDT Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCF160_VSV-G

Plasmid

#225963

Purpose

CMV-Intron-VSVG (env protein). Expresses VSV-G env for VLP production.

Depositor

Christof Fellmann

Article

Ramadoss et al Nat Commun. 2025 Nov 17;16(1):9883. doi:

Insert

CMV-Intron-VSVG (env protein)

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET iGluSnFR3 v857

Plasmid

#175186

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

iGluSnFR3 v857

Use

Synthetic Biology

Tags

His tag, T7 leader, Xpress epitope

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-BAZ1B

Plasmid

#65372

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BAZ1B (BAZ1B Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP7f

Plasmid

#104483

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7f

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-VPS13C-Cter

Plasmid

#232866

Purpose

Expresses the C-terminal fragments of VPS13C in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

the C-terminal fragments of VPS13C (VPS13C Human)

Tags

mCherry

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-ATG2C(VPS13C)

Plasmid

#232868

Purpose

Expresses the ATG2C domain of VPS13C in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

the ATG2C domain of VPS13C (VPS13C Human)

Tags

mCherry

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-PalmGRET

Plasmid

#158221

Purpose

BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.

Depositor

Charles P. Lai

Article

Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

Insert

PalmGFP-Nluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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tetO-AKT1

Plasmid

#170682

Purpose

doxycycline-inducible overexpression of AKT1

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

AKT1 (AKT1 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pICE-RNaseHI-D10R-E48R-NLS-mCherry

Plasmid

#60367

Purpose

Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

Depositor

Sébastien Britton, Patrick Calsou

Article

Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

Insert

RNase HI-D10R-E48R

Tags

NLS from SV40 T antigen and mCherry

Expression

Mammalian

Mutation

D10R+E48R; catalyticaly inactive

Promoter

CMV-tet

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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tetO-SOX9

Plasmid

#170703

Purpose

doxycycline-inducible overexpression of SOX9

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

SOX9 (SOX9 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-EWS-FLI1

Plasmid

#237636

Purpose

For overexpression of mEGFP-EWS-FLI1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EWS-FLI1 (EWSR1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

Plasmid

#85072

Purpose

Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor constitutively active (Q63L) (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Mutation

Q63L

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ASAP3-WPRE

Plasmid

#132331

Purpose

expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3

Use

AAV

Expression

Mammalian

Promoter

hSynapsin

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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