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pMM114
Plasmid#127211PurposeBeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDSDepositorInsertWUS2, IPT, sgRNA, Luc
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pet-MBP
Plasmid#209184PurposeExpression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coliDepositorTypeEmpty backboneUseTagsHisX6ExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV-TRE-WT human MyoD-T2A-dsRedExpress2
Plasmid#60628PurposeExpresses WT human MyoD and dsRedExpress2 in response to doxycyclineDepositorInsertVP64 mouse MyoD (MYOD1 Human)
UseLentiviralTagsExpressionMutationPromoterTREAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIVT-iCre
Plasmid#232773PurposeIVT template for making modRNA encoding iCreDepositorInsertiCre
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMM113
Plasmid#127210PurposeBeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDSDepositorInsertWUS2, AtSTM, sgRNA, Luc
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM119
Plasmid#127213PurposeBeYDV replicon with constitutive GFP expression, WUS2, sgRNA targeting PDSDepositorInsertGFP, WUS2, sgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-scramble
Plasmid#118349PurposeThis plasmid expresses scrabled shRNA for use as a control for shRNA HSFs plasmids.DepositorInsertscrambled shRNA
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
p6659 MSCV-IP N FlagHA HPV16E6
Plasmid#44152DepositorInsertHPV16E6
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCW57-RFP-P2A-MCS
Plasmid#78933PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro GFP-p62 D337, 338, 339A
Plasmid#38280DepositorInsertSequestosome-1 (Sqstm1 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationchanged D337A, D338A, D339APromoterAvailable sinceSept. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
H2B-MiniR
Plasmid#208328PurposeMiniR genetically encoded tag introduced after H2B; to be used with a MiniE probe peptide.DepositorInsertH2B-MiniR
UseTagsMiniRExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B-NCre
Plasmid#106368PurposeN-terminal part of the split-Cre SystemDepositorInsertNCre
UseCre/LoxTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-sypHy-RGECO
Plasmid#84078PurposeCombined fluorescent reporter for both presynaptic calcium influx and vesicle excoytosisDepositorInsertsypHy-RGECO
UseTagsExpressionMammalianMutationPromoterhSyn1Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-PKM2
Plasmid#64698PurposeExpression of GFP tagged PKM2 in mammalian cellsDepositorInsertPKM2 (PKM Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
GCaMP-AMPK-SPARK
Plasmid#226257PurposeDual reporter of Ca2+ levels and AMPK activityDepositorInsertGCaMP-AMPK-SPARK
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFREE_Zeo
Plasmid#92053PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionMutationPromoterpRhamBAD and ptetAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 GFP-h-PKD2
Plasmid#83451PurposeGFP-tagged PC2 for Eukaryotic expression (883)DepositorInsertPolycystin-2 (PKD2 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T MDM2 WT
Plasmid#16237DepositorInsertMDM2 (MDM2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mTurquoise2-CNA35
Plasmid#61602Purposebacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2DepositorInsertmTurqouise and CNA35
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI-SEP_NR2B
Plasmid#23998DepositorInsertNR2B (Grin2b Rat)
UseTagsNR2B signal sequence (aa1-31) and SEP (supereclip…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-jREX-GECO1
Plasmid#113941PurposeBacterial expression of red fluorescent Ca2+ sensor jREX-GECO1DepositorInsertjREX-GECO1
UseTags6xHis and Modified TorA MNNNDLFQASRRRFLAQLG[G to …ExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW57-GFP-2A-MCS
Plasmid#71783PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_eGFP_TIA1
Plasmid#106094Purposegene expression of EGFP-TIA1DepositorInsertTIA1 (TIA1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (GFP)
Plasmid#80924PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV5B-Flag-Smad3
Plasmid#11742DepositorInsertSMAD3 (SMAD3 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
PGK plasmid
Plasmid#169744PurposeUnregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterPGK promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro cPLA2-EGFP
Plasmid#162568PurposeUsed as a measure of cPLA2 activation in live cells with an EGFP tag.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Luc-pSUPER-Retro-GFP/Neo
Plasmid#22133DepositorInsertsynthethic oligonucleotide
UseRNAi and RetroviralTagsExpressionMammalianMutationsynthethic oligonucleotide encoding shRNA targeti…PromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-TAX1BP1
Plasmid#175761PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-TAX1BP1DepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 HaloTag-V5-TurboID
Plasmid#226474PurposeExpresses fusion of HaloTag and TurboID in mammalian cellsDepositorInsertTurboID
UseTagsHaloTag and V5ExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTetON-EGFP
Plasmid#89453PurposeTet/Dox-inducible expression of EGFP in mammalian cells (control)DepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
LZF1718: Cre-off hSyn-CheRiff-CFP (FAS)
Plasmid#104119PurposesynOptopatch constructDepositorInsertCheRiff-CFP
UseAdenoviralTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
hE-cadherin-pcDNA3
Plasmid#45769DepositorInserthE-cadherin (CDH1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion Halo-mAID
Plasmid#112852PurposeGeneral donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous proteinDepositorInsertHalo-mAID
UseSynthetic BiologyTagsExpressionMutationPromoterNoAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-gRNA-Puro
Plasmid#121121PurposeExpression of a gRNA together with a Puromycin resistanceDepositorInsertgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag1-NS5A
Plasmid#17646DepositorInsertHCV NS5A (POLY Hepatitis C virus)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pZLCv2-3xFLAG-dCas9-HA-2xNLS
Plasmid#106357PurposeEmpty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitationDepositorTypeEmpty backboneUseCRISPR and LentiviralTags3XFLAG and HAExpressionMammalianMutationPromoterU6Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1-Q61L
Plasmid#12981DepositorInsertRac1 constitutively active (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationQ61L Constitutively activePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
CAG FUCCI
Plasmid#175266PurposeBicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesisDepositorInsertFUCCI
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Centrin2 (Nigg UK185)
Plasmid#41147DepositorInsertCentrin-2 (CETN2 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
MBD2a pcDNA3.1
Plasmid#78141PurposeMammalian expression of MBD2aDepositorInsertMBD2a (MBD2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mAmetrine-CNA35
Plasmid#61604Purposebacterial expression of N-terminal fusion protein of CNA35 with mAmetrineDepositorInsertmAmetrine and CNA35
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTC-ApoE-Tet
Plasmid#85578PurposeSleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (ApoE.HCR.hAAT promoter ) in mouse liverDepositorInsertCreER
UseCre/LoxTagsExpressionMammalianMutationPromoterApoE.HCR.hAATAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LSSmOrange-CNA35
Plasmid#61605Purposebacterial expression of N-terminal fusion protein of CNA35 with LSSmOrangeDepositorInsertLSSmOrange and CNA35
UseTagsHis tagExpressionMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized
Plasmid#231602PurposeExpress circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized under CMV promoterDepositorInsertTornado-1xALFATag-5xSunTag_codon_optimized
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLAG SMG1 WT
Plasmid#199573PurposeExpresses FLAG-tagged WT SMG1DepositorInsertSMG1 (SMG1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1
Plasmid#175766PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1DepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Jonah-1
Plasmid#228899PurposeJonah-1 localizes to the outer cyst wall of Acanthamoeba tagged with eGFPDepositorInsertJonah-1
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwn promoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Leo-A
Plasmid#228901PurposeLeo-A localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFPDepositorInsertLeo-A
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwnAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only
