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We narrowed to 14,145 results for: TIM

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Showing: 8901 - 8950 of 14145 results

  1. pET15-EF2n2_mFAP2b

    Plasmid
    #159467
    Purpose
    Encodes for EF2n2_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF2n2_mFAP2b
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Available Since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159467-plasmid-map-sequence-id-316656

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  2. R777-E237 Hs.RASAL3

    Plasmid
    #70521
    Purpose
    Gateway ORF clone of codon optimized human RASAL3 [NM_022904.1] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASAL3 (RASAL3 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70521-plasmid-map-sequence-id-125970

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  3. pPB-CAG-PlatTET-wpA-pPuroTK

    Plasmid
    #214125
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    dCas9-TET
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214125-plasmid-map-sequence-id-424237

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  4. pcDNA4_ChR2opt_mKATE

    Plasmid
    #67958
    Purpose
    optical depolarization of mammalian cells
    Depositor
    Andreas Offenhäusser
    Insert
    Channelrhodopsin 2 opt
    Tags
    mKatushka
    Expression
    Mammalian
    Mutation
    synthetically produced for mammalian optimized co…
    Promoter
    CMV -TetON
    Available Since
    Sept. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    67958-plasmid-map-sequence-id-118415

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  5. pORFE0005

    Plasmid
    #112073
    Purpose
    AtDeadCAS9 D10A H840A without stop codon module CDS1ns
    Depositor
    Christian Chevalier
    Insert
    AtDeadCAS9 D10A H840A without stop codon
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112073-plasmid-map-sequence-id-217642

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  6. hSyn1-FLEX-hChR2-tdtomato

    Plasmid
    #41015
    Depositor
    Karel Svoboda
    Insert
    Channelrhodopsin-2
    Use
    Adenoviral
    Tags
    tdtomato
    Expression
    Mammalian
    Mutation
    codon-optimized (human)
    Promoter
    synapsin
    Available Since
    Nov. 6, 2012
    Availability
    Academic Institutions and Nonprofits only

  7. pKP1469

    Plasmid
    #160815
    Purpose
    Expression of ExbB protein; the exbB gene replaces tonB gene in BamHI site of pKP325; includes pBAD promoter and optimized RBS from pBAD24-derived pKP660 (ExbB, ExbD)
    Depositor
    Kathleen Postle
    Insert
    exbB
    Expression
    Bacterial
    Available Since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    160815-plasmid-map-sequence-id-316117

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  8. pET15b-ACP (from S. aureus)

    Plasmid
    #63686
    Purpose
    Inducible expression of Acyl Carrier Protein (ACP) from S. aureus
    Depositor
    Michael Johnson
    Insert
    ACP
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized
    Promoter
    T7
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. ATSR

    Plasmid
    #50052
    Purpose
    expresses t.gondii VWA+TSR1 domains in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2-ATSR
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A
    Promoter
    Chick beta-actin promoter
    Available Since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. proATSR

    Plasmid
    #50053
    Purpose
    Expresses T.gondii prodomain+VWA+TSR1 domains in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2-proATSR
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A
    Promoter
    chick beta-actin promoter
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. CMV-mt4xi-GCaMP6f

    Plasmid
    #192075
    Purpose
    Fluorescent reporter for mitochondrial calcium
    Depositor
    Timothy Ryan
    Insert
    4 repeats of improved COX8 signal peptide plus GCaMP6f
    Expression
    Mammalian
    Available Since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    192075-plasmid-map-sequence-id-378919

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  12. pLV-mScarlet-I

    Plasmid
    #159172
    Purpose
    To express a bright monomeric red FP for labelling and detection of mammalian cells
    Depositor
    Pantelis Tsoulfas
    Insert
    mScarlet-I
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Mutation
    Codons were optimized to Human codon usage
    Promoter
    CMV
    Available Since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159172-plasmid-map-sequence-id-311154

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  13. pCR2.1-InC

    Plasmid
    #83959
    Purpose
    Contains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.
    Depositor
    Kai-Shun Chen
    Insert
    Internal Control sequence
    Expression
    Bacterial
    Available Since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83959-plasmid-map-sequence-id-160468

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  14. R777-E139 Hs.NF1

    Plasmid
    #70423
    Purpose
    Gateway ORF clone of codon optimized human NF1 [NM_000267.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NF1 (NF1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70423-plasmid-map-sequence-id-125872

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  15. pBabepuro-PDGFB

    Plasmid
    #189611
    Purpose
    Retroviral expression vector for PDGFB. stimulates fibroblastic proliferation (Tchenio T, unpublished, 2008)
    Depositor
    Thierry Tchenio
    Insert
    PDGFB (PDGFB Human)
    Use
    Retroviral
    Promoter
    SV40 early promoter
    Available Since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    189611-plasmid-map-sequence-id-381075

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  16. pICSL4723_ERF922

    Plasmid
    #126883
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126883-plasmid-map-sequence-id-250052

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  17. pHH0103 SMURF2 WW domain #3

    Plasmid
    #104226
    Purpose
    Bacterial expression of WW domain #3 from SMURF2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF2 WW domain #3 (SMURF2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104226-plasmid-map-sequence-id-204295

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  18. pHH0103 SMURF2 WW domain #2

    Plasmid
    #104225
    Purpose
    Bacterial expression of WW domain #2 from SMURF2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF2 WW domain #2 (SMURF2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104225-plasmid-map-sequence-id-204192

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  19. pHH0103 SMURF2 WW domain #1

    Plasmid
    #104224
    Purpose
    Bacterial expression of WW domain #1 from SMURF2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF2 WW domain #1 (SMURF2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    104224-plasmid-map-sequence-id-201513

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  20. pHH0103 WBP4 WW domain #2

    Plasmid
    #104253
    Purpose
    Bacterial expression of WW domain #2 from WBP4
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WBP4 WW domain #2 (WBP4 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104253-plasmid-map-sequence-id-204383

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  21. R777-E309 Hs.SHC2

    Plasmid
    #70593
    Purpose
    Gateway ORF clone of codon optimized human SHC2 [NM_012435.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SHC2 (SHC2 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70593-plasmid-map-sequence-id-126042

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  22. pLSU1/t35s[TMV]:NLuc:NosT

    Plasmid
    #212181
    Purpose
    This binary vector expresses NanoLuciferase (Nluc) with the truncated 35sCAMV promoter and Tomato Mosaic Virus UTR
    Depositor
    Wayne Curtis
    Insert
    NanoLuc luciferase
    Expression
    Plant
    Mutation
    codon optimized for tomato
    Promoter
    truncated 35sCaMV
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    212181-plasmid-map-sequence-id-422345

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  23. pU-YLL058W-zr

    Plasmid
    #176565
    Purpose
    Expression of YLL058W from Zygosaccharomyces rouxii for auxotrophic selection in the absence of uracil
    Depositor
    Markus Ralser
    Insert
    YLL058W-zr
    Expression
    Yeast
    Mutation
    Codon optimised for expression in S.cerevisae
    Promoter
    TDH1(GAP)
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    176565-plasmid-map-sequence-id-350306

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  24. pENTR_Wheat_live_Cas9

    Plasmid
    #126880
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and BpiI sites for sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126880-plasmid-map-sequence-id-249730

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  25. pHH0103 TCERG1L WW domain #2

    Plasmid
    #104281
    Purpose
    Bacterial expression of WW domain #2 from TCERG1L
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    TCERG1L WW domain #2 (TCERG1L Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104281-plasmid-map-sequence-id-202574

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  26. pHH0103 STXBP4 WW domain

    Plasmid
    #104265
    Purpose
    Bacterial expression of WW domain from STXBP4
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    STXBP4 WW domain (STXBP4 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104265-plasmid-map-sequence-id-203219

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  27. pHH0103 PIN1L WW domain

    Plasmid
    #104269
    Purpose
    Bacterial expression of WW domain from PIN1L
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    PIN1L WW domain (PIN1P1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104269-plasmid-map-sequence-id-203206

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  28. pHH0103 SMURF1 WW domain #1

    Plasmid
    #104237
    Purpose
    Bacterial expression of WW domain #1 from SMURF1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF1 WW domain #1 (SMURF1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104237-plasmid-map-sequence-id-204802

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  29. pHH0103 SMURF1 WW domain #2

    Plasmid
    #104238
    Purpose
    Bacterial expression of WW domain #2 from SMURF1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF1 WW domain #2 (SMURF1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104238-plasmid-map-sequence-id-204801

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  30. R777-E311 Hs.SHC3

    Plasmid
    #70595
    Purpose
    Gateway ORF clone of codon optimized human SHC3 [NM_016848.5] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SHC3 (SHC3 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70595-plasmid-map-sequence-id-126044

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  31. R777-E238 Hs.RASAL3-nostop

    Plasmid
    #70522
    Purpose
    Gateway ORF clone of codon optimized human RASAL3 [NM_022904.1] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASAL3 (RASAL3 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70522-plasmid-map-sequence-id-125971

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  32. pFA6a-link-ymTurquoise2-Kan

    Plasmid
    #224092
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with Kan selection
    Depositor
    Bas Teusink
    Insert
    ymTurquoise2
    Tags
    ymTurquoise2
    Expression
    Yeast
    Available Since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224092-plasmid-map-sequence-id-453797

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  33. pFA6a-link-ymScarletI3-URA3

    Plasmid
    #224075
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with URA3 selection
    Depositor
    Bas Teusink
    Insert
    ymScarletI3
    Tags
    ymScarletI3
    Expression
    Yeast
    Available Since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224075-plasmid-map-sequence-id-460510

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  34. pFA6a-link-ymScarletI3-SpHis5

    Plasmid
    #224076
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with SpHis5 selection
    Depositor
    Bas Teusink
    Insert
    ymScarletI3
    Tags
    ymScarletI3
    Expression
    Yeast
    Available Since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224076-plasmid-map-sequence-id-460513

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  36. Pet28a_Synthetic_Human_H2A.1

    Plasmid
    #42634
    Depositor
    Joe Landry
    Insert
    Human H2A.1 (H2AC13 Human, Synthetic)
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Codon Optimized
    Promoter
    T7
    Available Since
    Feb. 25, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42634-plasmid-map-sequence-id-57749

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  37. pBS-L1PA1CH blast

    Plasmid
    #69612
    Purpose
    Expresses a blasticidin tagged codon optimized L1PA1
    Depositor
    Astrid Roy-Engel
    Insert
    L1PA1
    Tags
    blast cassette
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    69612-plasmid-map-sequence-id-123345

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  38. pFA6a-link-ymScarletI3-Kan

    Plasmid
    #224077
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with Kan selection
    Depositor
    Bas Teusink
    Insert
    ymScarletI3
    Tags
    ymScarletI3
    Expression
    Yeast
    Available Since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224077-plasmid-map-sequence-id-460512

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  39. psubCMV-mVEGF-D-WPRE

    Plasmid
    #119226
    Purpose
    Plasmid encodes full-length (unprocessed) murine VEGF-D to be used for stimulation of lymphangiogenesis in mice in vivo.
    Depositor
    Kari Alitalo
    Insert
    Vegfd (Vegfd Mouse)
    Use
    AAV
    Promoter
    CMV
    Available Since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    119226-plasmid-map-sequence-id-231680

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  40. ecALDH

    Plasmid
    #67921
    Purpose
    Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Insert
    Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
    Tags
    6x-His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    67921-plasmid-map-sequence-id-197668

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  41. R777-E140 Hs.NF1-nostop

    Plasmid
    #70424
    Purpose
    Gateway ORF clone of codon optimized human NF1 [NM_000267.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NF1 (NF1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70424-plasmid-map-sequence-id-125873

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  42. pFA6a-link-yemiRFP670-Kan

    Plasmid
    #224088
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized emiRFP670 with Kan selection
    Depositor
    Bas Teusink
    Insert
    yemiRFP670
    Tags
    yemiRFP670
    Expression
    Yeast
    Available Since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224088-plasmid-map-sequence-id-452414

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  43. pPB-CAG-dCas9-VPR-wpA-pPuroTK

    Plasmid
    #214127
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    dCas9-VPR
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214127-plasmid-map-sequence-id-424240

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  44. pFA6a-link-ymBaojin-SpHis5

    Plasmid
    #224082
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mBaojin with SpHis5 selection
    Depositor
    Bas Teusink
    Insert
    ymBaoJin
    Tags
    ymBaoJin
    Expression
    Yeast
    Available Since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224082-plasmid-map-sequence-id-452410

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  45. RABEP1A-c019

    Plasmid
    #175477
    Purpose
    Protein expression in insect cells. Entire CDS, M1-T862. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    RABEP1 (RABEP1 Human)
    Tags
    TEV-TwinStrep
    Expression
    Insect
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175477-plasmid-map-sequence-id-353250

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  46. pUbi_dCas9-BFP-KRAB-gRNA

    Plasmid
    #221503
    Purpose
    CRISPRi machinery fish codon optimized expressing plasmid
    Depositor
    Alessandro Ori
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Mutation
    D10A and H839A point mutations
    Promoter
    Zebrafish Ubiquitin promoter
    Available Since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221503-plasmid-map-sequence-id-439415

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  47. pHN872

    Plasmid
    #178830
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A
    Tags
    GST, His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178830-plasmid-map-sequence-id-351931

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  48. pJJR81

    Plasmid
    #75029
    Purpose
    tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Mike Boxem
    Insert
    tagBFP2^SEC^3xFlag
    Use
    CRISPR
    Tags
    3xFLAG and C. elegans codon optimized tagBFP2
    Expression
    Worm
    Available Since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    75029-plasmid-map-sequence-id-141917

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  49. pBHM2642

    Plasmid
    #229526
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229526-plasmid-map-sequence-id-459924

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  50. pFA6a-link-ymiRFP680-URA3

    Plasmid
    #224102
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized miRFP680 with URA3 selection
    Depositor
    Bas Teusink
    Insert
    ymiRFP680
    Tags
    ymiRFP680
    Expression
    Yeast
    Available Since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    224102-plasmid-map-sequence-id-453896

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