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pLENTI CMV GFP-TREX1 delta N (aa236-314) BLAST

Plasmid

#164235

Purpose

lentiviral plasmid for GFP-TREX1 delta N (aa236-314)

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deletion of amino acids 1-234

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-D2s-L-V1

Plasmid

#19967

Depositor

Jonathan Javitch

Article

Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

Insert

D2 short variant-N terminal Venus (DRD2 Human, Aequorea Victoria)

Tags

Flag and N terminal Venus

Expression

Mammalian

Available Since

Jan. 27, 2009

Availability

Academic Institutions and Nonprofits only
pSL0812 (pCRISPR(BbsI)_Vch)

Plasmid

#200898

Purpose

Expresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCRISPR (entry)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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TRAF6mut-UTR

Plasmid

#15094

Depositor

David Baltimore

Article

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

TRAF6 3'UTR (TRAF6 Human)

Use

Luciferase

Expression

Mammalian

Mutation

4-bp substitutions in the miR-146 target sites

Available Since

June 6, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-ERBB4 (kinase dead)

Plasmid

#29533

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

changed lysine 751 to methionine

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

Plasmid

#200910

Purpose

3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

Depositor

Debojyoti Chakraborty

Insert

3XHA_den1FnCas9_KRAB_eGFP

Use

CRISPR

Tags

3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP

Expression

Mammalian

Mutation

Changed Glutamic Acid to Arginine at 1369 position

Promoter

CBH

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pQlinkH-PTPIP51(36-235)

Plasmid

#170533

Purpose

Expresses Cleavable His-tagged PTPIP51_ΔTPR domain (36-235) in E. Coli

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)

Tags

His tag

Expression

Bacterial

Promoter

T5 promoter

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat

Plasmid

#133779

Purpose

Expresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Insert

Isocitrate dehydrogenase 1 (IDH1 Human)

Tags

Myc and FLAG

Expression

Mammalian

Mutation

Arginine is mutated to histidine at amino acid po…

Available Since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Tollip M240A,F241A

Plasmid

#178051

Purpose

GFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Methionine 240 to Alanine and changed Phe…

Promoter

CMV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF1-ERBB4 (kinase dead)

Plasmid

#29544

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

changed lysine 751 to methionine

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pCI-neo-mApg5

Plasmid

#22956

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pEBB COMMD2 Flag

Plasmid

#74894

Purpose

Express COMMD2 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD2 (COMMD2 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pIRESneo2 mycBioID-Tollip

Plasmid

#178054

Purpose

myc-BioID fused to human Tollip cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Tags

mycBioID

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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1375_pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-CB-SACas9-HA-OLLAS-spA

Plasmid

#109311

Purpose

Plasmid for AAV SaCas9 Mammalian Expression with a gRNA against Ldlr

Depositor

William Lagor

Article

Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

Insert

Ldlr gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pESC-URA-hPOLISc

Plasmid

#60819

Purpose

to express human Pol iota recoded to yeast codons in yeast

Depositor

Youri Pavlov

Article

Makarova et al PLoS One. 2011 Jan 31;6(1):e16612. doi:

Insert

POLI (POLI Human)

Tags

c-myc

Expression

Yeast

Mutation

optimized to yeast codon usage

Promoter

GAL1

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pKnockinDonor-humanCTCF-miniAID-mClover3

Plasmid

#136883

Purpose

knockin donor vector of miniAID-mClover3 to C-terminus of endogenous human CTCF

Depositor

Chunliang Li

Article

Hyle et al Nucleic Acids Res. 2019 Jul 26;47(13):66

Insert

CTCF-knockin-homology arm-miniAID-mClover3

Tags

miniAID-mClover3

Expression

Mammalian

Promoter

no promoter

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-MED8

Plasmid

#15406

Depositor

Joan Conaway, Ronald Conaway

Article

Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

Insert

MED8 (MED8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
K15/LacZ

Plasmid

#44265

Depositor

George Cotsarelis

Article

Liu et al J Invest Dermatol. 2003 Nov;121(5):963-8

Insert

K15 promoter (-4.8) (Krt15 Mouse)

Use

Mouse Targeting; Β-galactosidase

Tags

LacZ

Expression

Mammalian

Mutation

Contains the murine K15 promoter (-4.8) fragment

Promoter

murine K15 promoter (-4.8) fragment

Available Since

April 11, 2013

Availability

Academic Institutions and Nonprofits only
pSLIK 3xFLAG-KIF20A zeo

Plasmid

#192322

Purpose

Lentiviral expression vector for an inducible 3xFLAG-KIF20A

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KIF20A (KIF20A Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-KIF20A

Plasmid

#192297

Purpose

Gateway entry vector for an inducible 3xFLAG-KIF20A

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KIF20A (KIF20A Human)

Use

Gateway entry vector

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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