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pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry

Plasmid

#181853

Purpose

mCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-KMS

Plasmid

#188038

Purpose

Polycistronic human KLF4, MYC and SOX2 were cloned into the pMXs vector

Depositor

Young Tang

Article

Wang et al Stem Cell Res. 2017 Dec;25:88-97. doi: 1

Insert

KLF4, MYC, and SOX2 separated by 2A sequences (KLF4, MYC, SOX2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-FXN

Plasmid

#23620

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FXN (FXN Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAPK8

Plasmid

#23420

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK8 (MAPK8 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLenti CMV Puro DEST (w118-1) FAM149A

Plasmid

#107510

Purpose

Expressing FAM149a, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

FAM149A (FAM149A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAPK10

Plasmid

#23649

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK10 (MAPK10 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
TMEM230-M4/X184PG-IRES(isoform 2)

Plasmid

#99568

Purpose

mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Expression

Mammalian

Mutation

M4/X184PG

Promoter

CMV

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM230-M3/Y92C-IRES(isoform 2)

Plasmid

#99567

Purpose

mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Expression

Mammalian

Mutation

M3/Y92C

Promoter

CMV

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM230-M2/X184W-IRES(isoform 2)

Plasmid

#99566

Purpose

mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Expression

Mammalian

Mutation

M2/X184W

Promoter

CMV

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM230-M1/R141L-IRES(isoform 2)

Plasmid

#99565

Purpose

mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Expression

Mammalian

Mutation

M1/R141L

Promoter

CMV

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-GNE

Plasmid

#23448

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GNE (GNE Human)

Use

Gateway donor vector

Mutation

D407G

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pHAGE2-TetOminiCMV-OKM

Plasmid

#136554

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Klf4, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-T2A-Klf4-E2A-cMyc (Pou5f1, Myc, Klf4 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-KSM

Plasmid

#136553

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Klf4, Sox2, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Klf4-IRES-Sox2-E2A-cMyc (Klf4, Sox2, Myc Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-OSM

Plasmid

#136555

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-P2A-Sox2-E2A-cMyc (Myc, Pou5f1, Sox2 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL

Plasmid

#73210

Purpose

Expresses myc-dL5(E52D)-mCer3 fusion protein in peroxisomes of mammalian cells with a C-terminal PTS (SKL). (MBIC5, dL5**, FAP)

Depositor

Marcel Bruchez

Article

Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

Insert

KozATG-myc-dL5-2XG4S-mCer3-SKL (MYC Synthetic, Human)

Tags

The FAP and mCerulean3 are fused with 2 copies of…

Expression

Mammalian

Mutation

The dL5** FAP is E50D, L89S, also known as E52D/L…

Promoter

CMV

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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TMEM230-M2/X184W-IRES(isoform 1)

Plasmid

#99560

Purpose

mammalian expression of mutant TMEM230 (isoform 1) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Expression

Mammalian

Mutation

M2/X184W

Promoter

CMV

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM230-M1/R141L-IRES(isoform 1)

Plasmid

#99559

Purpose

mammalian expression of mutant TMEM230 (isoform 1) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Expression

Mammalian

Mutation

M1/R141L

Promoter

CMV

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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CACNA1D (2of2) or cav1.3b_L (OZ547)

Plasmid

#28072

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting CACNA1D (2of2) or cav1.3b (cacna1db Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
CACNA1D (2of2) or cav1.3b_R (OZ548)

Plasmid

#28073

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting CACNA1D (2of2) or cav1.3b (cacna1db Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag DYRK4

Plasmid

#20480

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

DYRK4 (DYRK4 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only