Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 12,214 results for: LEA

Clear keywords
Showing: 3561 - 3580 of 12214 results

  1. pcDNA3.1(+)-PM-CaNARi

    Plasmid
    #64850
    Purpose
    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.
    Depositor
    Jin Zhang
    Publication
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-CaNARi
    Tags
    Cerulean, N-terminal targeting sequence from Lyn …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    64850-plasmid-map-sequence-id-109675

    Enlarge

  2. pcDNA-NES-ZapCmR2

    Plasmid
    #59015
    Purpose
    Cytosolic zinc-binding FRET biosensor
    Depositor
    Amy Palmer
    Publication
    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor
    Tags
    Nuclear Exclusion Signal
    Expression
    Mammalian
    Mutation
    See comment
    Promoter
    CMV
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pCAG-IRES2-p75NTR-GFP

    Plasmid
    #182947
    Purpose
    To express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-Terminal
    Depositor
    Pantelis Tsoulfas
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p75NTR-mGFP
    Tags
    HA tag and mEGFP
    Expression
    Mammalian
    Mutation
    The leader sequence is from preprotrypin
    Promoter
    CAG
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    182947-plasmid-map-sequence-id-360205

    Enlarge

  9. SND1 (SND1A-c003)

    Plasmid
    #98242
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SND1A (SND1 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00567:TUDOR 677-799
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98242-plasmid-map-sequence-id-193420

    Enlarge

  10. p3.2mar-GFP

    Plasmid
    #63694
    Purpose
    3.2 kb marine armor plate enhancer driving GFP reporter
    Depositor
    David Kingsley
    Publication
    O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3.2 kb marine stickleback armor plate enhancer
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    hsp70
    Available since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    63694-plasmid-map-sequence-id-106193

    Enlarge

  11. pEMS1611

    Plasmid
    #29276
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple139 (NR2E1 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    29276-plasmid-map-sequence-id-13691

    Enlarge

  12. pEMS1612

    Plasmid
    #29277
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple140 (NR2E1 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    29277-plasmid-map-sequence-id-13692

    Enlarge

  13. pBJG1 T7-8xHis-OGT D554N

    Plasmid
    #154279
    Purpose
    Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Publication
    Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Aspartate 554 changed to Asparagine- bacterial co…
    Available since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    154279-plasmid-map-sequence-id-304834

    Enlarge

  17. pcDNA3-LYRa11-RBD-8h

    Plasmid
    #161822
    Purpose
    Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11
    Depositor
    Erik Procko
    Publication
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T324-D522)
    Promoter
    CMV
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    161822-plasmid-map-sequence-id-316758

    Enlarge

  18. His-ZZ-TEV-SNAPf-DYNC2H1

    Plasmid
    #132524
    Purpose
    Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments
    Depositor
    Andrew Carter
    Publication
    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1
    Insert
    DYNC2H1 (DYNC2H1 Human)
    Tags
    8x His, Linker-TEV-linker-TEV, Precission cleavag…
    Expression
    Insect
    Mutation
    Codon optimised for Sf9 expression
    Available since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    132524-plasmid-map-sequence-id-261476

    Enlarge

Showing: 3561 - 3580 of 12214 results