We narrowed to 15,995 results for: LEA

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pXCX-sGFAP-LMO-IRES-tdTomato

Plasmid

#178312

Purpose

Express the bacterial lactate 2-monooxygenase (LMO) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

Lactate 2-monooxygenase

Use

Adenoviral

Tags

tdTomato

Expression

Mammalian

Promoter

sGFAP

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJH807_pJH_LldR_Pcon-sfGFP.2TAG

Plasmid

#163738

Purpose

Constitutive expression of green fluorescent protein encoding two stop codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.2TAG

Expression

Bacterial

Mutation

gfp.N39TAG,Y135TAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH809_pJH_LldR_Pcon-sfGFP.10TAG

Plasmid

#163739

Purpose

Constitutive expression of green fluorescent protein encoding ten stop codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.10TAG

Expression

Bacterial

Mutation

gfp with first 10 leucine codons mutated to amber

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-1

Plasmid

#164865

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-1

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pPS832

Plasmid

#1357

Depositor

Pamela Silver

Article

Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

Insert

GSP1 (GSP1 Budding Yeast)

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pc3XB-ZF5

Plasmid

#13104

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF5

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pGEXndoE(E662Q)

Plasmid

#47716

Purpose

Expresses GST-EndoE(E662Q) from E. faecalis in E. coli

Depositor

Mattias Collin, Vincent Fischetti

Article

Collin et al J Biol Chem. 2004 May 21;279(21):22558-7

Insert

ndoE

Tags

GST-EndoE(E186Q) and Glutathione S-transferase

Expression

Bacterial

Mutation

Changed Glu-662 to Gln

Promoter

tac

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pRS423-SgH

Plasmid

#163972

Purpose

Helper plasmid for cloning gRNAs under the control of the SNR52 promoter.

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR Bmi1-C

Plasmid

#30511

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

Insert

Bmi1 (Bmi1 Mouse)

Use

Gateway - donor vector

Mutation

NO STOP

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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Hy_pPepck1 eGFP SV40

Plasmid

#132644

Purpose

Expresses eGFP mRNA from the Drosophila Pepck1 promoter

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Expression

Insect

Promoter

Pepck1

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NEO-CAG-BFP-pA-GFP-pA

Plasmid

#149344

Purpose

Vector for targeting the Replace reporter to the AAVS1 locus

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

BFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-HIRAmut-polyA

Plasmid

#59782

Purpose

This Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

HIRA

Expression

Mammalian

Mutation

Change from 'GATCGAAGTTTG' to 'GAc…

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF10

Plasmid

#13109

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF10

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pTEI134

Plasmid

#126071

Purpose

Vector for expression of plastid targeted YFP1-10 (T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

YFP1-10

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0007

Plasmid

#117534

Purpose

Level0 Golden Gate part: CDS, LbCas12a

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

LbCas12a (with stop codon)

Use

CRISPR

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF64

Plasmid

#13200

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF64

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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pBSKΔB-CBh-Cas9n-pA

Plasmid

#82617

Purpose

Expresses Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

SpCas9n

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF63

Plasmid

#13199

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF63

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF2

Plasmid

#13101

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF2

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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Hy_pMtnA nLuc SV40

Plasmid

#132654

Purpose

Expresses nanoLuciferase (nLuc) mRNA from the Drosophila Metallothionein A promoter

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Insect

Promoter

MtnA

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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