We narrowed to 22,373 results for: Sis

1. pUB-nSypHer (NLS:sypHer:NLS)

   Plasmid

   #84731

   Purpose

   Expresses sypHer in plant cells-biosensor for pH targeted to Nuclei

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Insert

   nSypHer
   

   Use

   Tags

   NLS (nuclear localisation signal and NLS (nuclear…

   Expression

   Plant

   Mutation

   Promoter

   ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT/Caggs-NRAS G12V-IRES-ASK1

   Plasmid

   #221074

   Purpose

   Expresses NRAS G12V and ASK1 in mammalian cells

   Depositor

   Hidenori Ichijo

   Article

   Odawara et al Commun Biol. 2024 Jun 5;7(1):691. doi: 1

   Inserts

   NRAS (NRAS Human)
   ASK1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUBQ10:AtFLS2

   Plasmid

   #202186

   Purpose

   Express Arabidopsis thaliana FLS2 gene under UBQ10 promoter

   Depositor

   Gitta Coaker

   Article

   Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

   Insert

   Flagellin sensing 2 (FLS2 Mustard Weed)
   

   Use

   Tags

   HA

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. cfmTurquoise-N

   Plasmid

   #139284

   Purpose

   Cys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.

   Depositor

   Ikuo Wada

   Article

   Omae et al Transfusion. 2019 Jul;59(7):2429-2435. d

   Insert

   cfmTurquoise
   

   Use

   Tags

   Multi-cloning sites

   Expression

   Mammalian

   Mutation

   Cys48Ser and Cys70/Met

   Promoter

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGMEH-T38M-750-luxAB-DAS+4

   Plasmid

   #49520

   Purpose

   Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomally

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   T38M-P750-luxAB-DAS+4
   

   Use

   Tags

   DAS+4 (AANDENYSENYADAS)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. SEP-Nanoluc-cyOFP1

   Plasmid

   #141082

   Purpose

   prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   SEP-Nanoluc-cyOFP1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deletion of amino acids DISGG from positions 673 …

   Promoter

   EF1α

   Available since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCold I-Hero7

   Plasmid

   #187926

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero7

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero7 (SERF2 Human)
   

   Use

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCold I-Hero45

   Plasmid

   #187931

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero45

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero45 (SERBP1 Human)
   

   Use

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA-T-intron(Neo)-G(LNL)

   Plasmid

   #36908

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   T (i.e., ATG, start codon as a first exon)
   GFP
   beta-globin intron
   Neo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides before nucleotide 275 and ins…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. AID-BFP-loxP_myo2_neoR

    Plasmid

    #194054

    Purpose

    Dual-selection cassette plasmid for knocking-in AID-BFP into the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Use

    Tags

    bfp

    Expression

    Worm

    Mutation

    Promoter

    None

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. HBT-CPK32-Flag

    Plasmid

    #107432

    Purpose

    For Arabidopsis mesophyll protoplast transient expression assay

    Depositor

    Jen Sheen

    Article

    Liu et al Nature. 2017 May 18;545(7654):311-316. d

    Insert

    CPK32 (CPK32 Mustard Weed)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEM-PH-citrine

    Plasmid

    #131406

    Purpose

    Purpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrine

    Depositor

    Lars Hufnagel

    Article

    Guignard et al Science. 2020 Jul 10;369(6500). pii: 369

    Insert

    PH
    

    Use

    In vitro transcription

    Tags

    citrine yellow fluorescent protein

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS

    Plasmid

    #127365

    Purpose

    Binary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Plant

    Mutation

    R118G

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZJQIBEBT003

    Plasmid

    #140053

    Purpose

    The pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.

    Depositor

    Jia Zhang

    Article

    Zhang et al ACS Synth Biol. 2020 Apr 17;9(4):940-952

    Insert

    eMutS-L157C-G233C-CBM-EGFP
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    L157C, G233C

    Promoter

    T7

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. sox10:PDGFRA wild-type

    Plasmid

    #98696

    Purpose

    I-SceI meganuclease-mediated human PDGFRA wild-type transgenesis in zebrafish

    Depositor

    Thomas Look

    Article

    Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

    Insert

    PDGFRA (PDGFRA Human)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT2Apuro-TRE-synPCB2.0

    Plasmid

    #139481

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
    

    Use

    Tol2 transposon donor vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet responsive element

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. p35S::IRE1a

    Plasmid

    #135232

    Purpose

    constitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)

    Depositor

    Armin Djamei

    Article

    Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

    Insert

    IRE1a (IRE1A Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFRT-TODestFLAGHA_RBPMS_KDR_HindIII

    Plasmid

    #65662

    Purpose

    FLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    introduced HindIII site (final goal to exchange F…

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP

    Plasmid

    #127363

    Purpose

    Binary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    R118G

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCA-G-intron(Neo)-T(FLLFLNL)

    Plasmid

    #40020

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tdT-3Myc
    beta-globin intron
    Neo
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta globin promoter and CMV enhance…

    Available since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge