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pMCh-1548

Plasmid

#82435

Purpose

Plasmid for expression of HA-tagged Nematostella vectensis AGO2 in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvAGO2

Tags

HA

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA7_no stop

Plasmid

#83872

Purpose

Gateway donor vector for IAA7 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA7 (IAA7 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pGMCKq20-T38S38-0X

Plasmid

#49522

Purpose

Kanamycin resistant; contains qtag20 and constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

constitutive TetR38

Expression

Bacterial

Promoter

Ptb38

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1/V5-His TOPO TA mouse Mns1

Plasmid

#42899

Depositor

Jeremy Wang

Article

Zhou et al PLoS Genet. 2012;8(3):e1002516. doi: 10.

Insert

Mns1 (Mns1 Mouse)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only
pUC19_CRISPR_DpmrA

Plasmid

#160903

Purpose

CRISPR_Cas9, lambda red recombineering

Depositor

Anne-Catrin Uhlemann

Article

McConville et al Cell Rep. 2020 Oct 27;33(4):108313. doi:

Inserts

Cas9
Lambda Red Recombineering Genes
Zeocin Resistance Cassette

Promoter

araBAD

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pWaldo-GFPe_PepTSo

Plasmid

#58334

Purpose

Expresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged protein

Depositor

So Iwata, Simon Newstead

Article

Newstead et al EMBO J. 2011 Jan 19;30(2):417-26. doi: 1

Insert

PepTso

Tags

GFP-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pDOC-GG

Plasmid

#149377

Purpose

Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring

Depositor

Mark Pallen

Article

Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

Inserts

Fragment containing a kanamycin resistance cassette
Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
Fragment containing an I-SceI recognition sequence

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-MCS_PGK-GpNLuc

Plasmid

#70185

Purpose

Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Article

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pGMCtN-TSC10M1-sspB

Plasmid

#49521

Purpose

Nourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

TSC10M1-sspB

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pDGE334

Plasmid

#153243

Purpose

M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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OSCA2.3

Plasmid

#136595

Purpose

Expresses the mechanosensitive channel OSCA2.3 in mammalian cells

Depositor

Ardem Patapoutian

Article

Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

Insert

OSCA2.3 (HYP1 Mustard Weed)

Tags

pIRES2-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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ARL13B-(ΔGTPase)- GFP

Plasmid

#246843

Purpose

Express Arl13B (resistance to sgRNA) with deletion of GTPase, tagged with GFP

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deleted GTPase domain

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHsp18.2 (GB0153)

Plasmid

#68171

Purpose

Provides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtHsp18.2 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-MCS_PGK-OgNLuc

Plasmid

#70186

Purpose

Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Article

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pSH124-ssr

Plasmid

#198025

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Ap/Cb resistance cassette

Expression

Bacterial

Promoter

ApR promoter

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSH824-ssr

Plasmid

#198027

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Apr resistance cassette

Expression

Bacterial

Promoter

AprR promoter

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHyg-AID*-6HA

Plasmid

#99520

Purpose

C-terminal AID*-6HA degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

6xHA

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSH424-ssr

Plasmid

#198026

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Sm resistance cassette

Expression

Bacterial

Promoter

SmR promoter

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pT7C-BAG2

Plasmid

#177654

Purpose

Recombinant protein synthesis of human BAG2

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

BAG2 (BAG2 Human)

Tags

6xHis-Thrombin

Expression

Bacterial

Promoter

T7

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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SUPV3L1-HA WT

Plasmid

#232348

Purpose

Expresses human SUPV3L1/SUV3 in mammalian cells with HA tag

Depositor

Samantha Lewis

Article

Begeman et al Sci Adv. 2025 Apr 18;11(16):eads6830. do

Insert

SUPV3L1 (SUPV3L1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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