2,162 results

1. pPRISM-Cre-gcry-eGFP

   Plasmid

   #117791

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   Cre
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPRISM-TagRFP-gcry-mRFP

   Plasmid

   #117766

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   TagRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2vegf165

   Plasmid

   #22417

   Depositor

   Nathan Lawson

   Article

   Lawson et al Dev Cell. 2002 Jul . 3(1):127-36.

   Insert

   vegf (vegfaa Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPRISM-Stop-cmlc2-eGFP

   Plasmid

   #117776

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   3x3 Stop sequence
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGTag-NLS-eGFP-B-actin

   Plasmid

   #117812

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. gfap-EGFP donor

   Plasmid

   #65564

   Purpose

   Intron targeting-mediated EGFP knockin at the zebrafish gfap locus

   Depositor

   Jiu-lin Du

   Article

   Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

   Insert

   gfap-EGFP (gfap Zebrafish)
   

   Use

   Zebrafish injection

   Tags

   Expression

   Mutation

   Promoter

   none

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pXTm-zebrafish Tspan4a

   Plasmid

   #210327

   Purpose

   Expresses Tetraspanin-4a (TSPAN4a) as migrasome marker

   Depositor

   Li Yu

   Article

   Jiang et al Nat Cell Biol. 2019 Aug;21(8):966-977. d

   Insert

   Tetraspanin 4a (tspan4a Zebrafish)
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT2-cryR;dll4P1Egfp

   Plasmid

   #90160

   Purpose

   contains dll4 specific gene promoter driving expression of eGFP

   Depositor

   Nathan Lawson

   Article

   Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

   Insert

   dll4P1Egfp (dll4 Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds

   Plasmid

   #119074

   Purpose

   Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   ubb:MCP-p65-HSF1-2a-Cerulean
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Zebrafish ubiquitin promoter

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds

    Plasmid

    #119075

    Purpose

    Zebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    ubb:Com-KRAB-2a-Cerulean
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish ubiquitin promoter

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVC-Ds-DrU6a:sgRNA-com-Ds

    Plasmid

    #119077

    Purpose

    To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:2xCom-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVC-Ds-DrU6a:sgRNA-MS2-Ds

    Plasmid

    #119076

    Purpose

    To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:2xMS2-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. actc1b-lamp1-mKate2

    Plasmid

    #109507

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to lamp1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    lamp1 (lamp1b Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBluescript goosecoid

    Plasmid

    #16935

    Depositor

    John Postlethwait

    Article

    Thisse et al Dev Biol. 1994 Aug . 164(2):420-9.

    Insert

    goosecoid (gsc Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

15. GBH-2aG4-gcryBFP.3

    Plasmid

    #188015

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; gamma crystallin promotor

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GBH-R-gcryGFP.3

    Plasmid

    #188000

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted mRFP protein trap; secondary marker gamma crystallin GFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; gamma crystallin promotor

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT7goldRNA

    Plasmid

    #47930

    Purpose

    In vitro transcription of golden gRNA

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    golden gRNA (slc24a5 Zebrafish)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMTB2-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80066

    Purpose

    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Danio rerio cryaba

    Plasmid

    #47620

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha Ba-crystallin (cryaba Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TALEN1297

    Plasmid

    #32279

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting hey2 (hey2 Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge