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pHB-3

Plasmid

#140956

Purpose

For PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.

Depositor

Kang Zhou

Article

Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

Insert

phaCAB

Expression

Bacterial

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT-MBI (JBEI-3085)

Plasmid

#87950

Purpose

the overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).

Depositor

Taek Soon Lee

Article

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Insert

MevT-MBI

Expression

Bacterial

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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KO plasmid

Plasmid

#135970

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MET-HYGRO

Plasmid

#218113

Purpose

Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH-GFPminimizer

Plasmid

#202488

Purpose

Expresses the nanobody GFP minimizer with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody GFP minimizer

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pVH41-Tier1-PhCMV-GLuc

Plasmid

#169544

Purpose

Tier-1 vector encoding PhCMV-driven gaussia luciferase (PhCMV-GLuc-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven Gaussia Luciferase

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pPPC009.106

Plasmid

#171142

Purpose

Expression of J106 scRNA on pBBR1-KmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

J106 scRNA

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRA1EGFP

Plasmid

#193791

Purpose

Expresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

EGFP

Expression

Bacterial

Promoter

PR promoter

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pY2-MET-IV

Plasmid

#218117

Purpose

MET-HYGRO plasmid (218133) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pY2-LYS-IV

Plasmid

#218118

Purpose

LYS-Leu2 plasmid (218114) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH72

Plasmid

#202487

Purpose

Expresses the nanobody VHH72 with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody VHH72

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCas2C

Plasmid

#82374

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Promoter

PEZ3

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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ptrc99a-pVhb-tdTER-fdh

Plasmid

#215649

Purpose

pBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoter

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Inserts

trans-enoyl-CoA reductase
formate dehydrogenase

Expression

Bacterial

Promoter

Vhb

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET30b_T7Sav

Plasmid

#138588

Purpose

T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori

Depositor

Markus Jeschek

Article

Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

Insert

T7-tagged streptavidin variant with native C-terminus

Use

Synthetic Biology

Tags

T7-tag

Expression

Bacterial

Promoter

PT7

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pVH231-Tier1-PmPGK1-mTagBFP2

Plasmid

#169520

Purpose

Tier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven monomeric Tag blue fluorescent protein 2

Expression

Mammalian

Promoter

PPGK

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCORE-Kp53

Plasmid

#72241

Purpose

Contains the CORE cassette with KanMX4 and human p53 gene markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

p53 (TP53 Human)
kanMX4

Expression

Bacterial

Mutation

V122A mutation

Available Since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pRC3EGFP

Plasmid

#193790

Purpose

Expresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

EGFP

Expression

Bacterial

Promoter

PR promoter

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTCW012

Plasmid

#83527

Purpose

Cloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminator

Depositor

Claudia Vickers

Article

Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

pFUS1J2

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pVH233-Tier1-PmPGK1-eYFP

Plasmid

#169522

Purpose

Tier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven enhanced yellow fluorescent protein

Expression

Mammalian

Promoter

PPGK

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCORE-Hp53

Plasmid

#72242

Purpose

Contains the CORE cassette with hyg and human p53 gene markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

p53 (TP53 Human)
hyg

Expression

Bacterial

Mutation

V122A mutation

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-Casp2

Plasmid

#50023

Purpose

pENTR vector for mouse Caspase-2

Depositor

Jamie Davies

Article

Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

Insert

Casp2 (Casp2 Mouse)

Use

Gateway Cloning

Available Since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only
pBTRCk-pVhb-maACR

Plasmid

#215651

Purpose

pBBR1 Origin, KanR, with maACR gene under Vhb promoter

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Insert

acyl-CoA reductase

Tags

Maltose binding protein and His tag

Expression

Bacterial

Promoter

Vhb

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC-pVhb-vfFadBA

Plasmid

#215650

Purpose

pACYC Origin, CmR, with vfFadBA gene under Vhb promoter

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Insert

thiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)

Expression

Bacterial

Promoter

Vhb

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pY3

Plasmid

#218116

Purpose

Activity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pY3-LYS-IV

Plasmid

#218119

Purpose

LYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pNVLTv2-pvdIJD-Ptrc-base

Plasmid

#169243

Purpose

Suicide vector for integrating expression cassette in P. putida (IPTG induction)

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

lacI

Expression

Bacterial

Promoter

lacIq

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCas5

Plasmid

#82378

Purpose

sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PJ23119

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GYB

Plasmid

#160927

Purpose

Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Inserts

BFP
sfGFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBbE1k-2

Plasmid

#59945

Purpose

dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

Depositor

Taek Soon Lee

Article

Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

Inserts

dihydropterine reductase
pterin-4a-carbinolamine dehydratase

Promoter

pTrc

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pRS416-Aga1p-FAPB2.3.6

Plasmid

#158147

Purpose

Yeast display reporter for non-display yeast strains (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p-FAPB2.3.6

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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