Addgene

We narrowed to 3,272 results for: aaas

Showing: 681 - 720 of 3272 results

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pU6-pegRNA-SNCA-A53T

Plasmid

#181738

Purpose

Transiently expressing a pegRNA to introduce SNCA-A53T mutation in human cells

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for SNCA-A53T (SNCA Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-SNCA-A30P

Plasmid

#180016

Purpose

Transiently expressing a pegRNA to introduce SNCA-A30P mutation in human cells

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for SNCA-A30P (SNCA Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NLS-IRES-hrGFP

Plasmid

#107543

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NLS (APP Human)

Use

AAV

Tags

Expression

Mutation

None

Promoter

CMV

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV Syn Barcode14

Plasmid

#226188

Purpose

Expression mapping

Depositor

Scott Sternson

Insert

Syn Barcode14

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV Syn Barcode18

Plasmid

#226191

Purpose

Expression mapping

Depositor

Scott Sternson

Insert

Syn Barcode18

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13206

Plasmid

#219881

Purpose

The base plasmid of TUNEYALI for TF16

Depositor

Irina Borodina

Insert

Contains gRNA targeting TF16 (YALI1_B11759g) and homologous arm matching TF16

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pHK352.3

Plasmid

#235473

Purpose

Message phagemid carrying sgRNA3 (prom. J23110, backbone RSF1030)

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

sgRNA3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK302.6

Plasmid

#235471

Purpose

Message phagemid carrying sgRNA6 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

sgRNA6

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK052.3

Plasmid

#235472

Purpose

Message phagemid carrying sgRNA3 (prom. J23119, backbone RSF1030)

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

sgRNA3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK002.1

Plasmid

#235460

Purpose

Message phagemid carrying sgRNA1 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

sgRNA1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK002.2

Plasmid

#235461

Purpose

Message phagemid carrying sgRNA2 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

sgRNA2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK002.3

Plasmid

#235462

Purpose

Message phagemid carrying sgRNA3 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

sgRNA3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK002.4

Plasmid

#235463

Purpose

Message phagemid carrying sgRNA4 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

sgRNA4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK002.5

Plasmid

#235464

Purpose

Message phagemid carrying sgRNA5 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

sgRNA5

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK002.6

Plasmid

#235465

Purpose

Message phagemid carrying sgRNA6 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

sgRNA6

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK302.1

Plasmid

#235466

Purpose

Message phagemid carrying sgRNA1 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

sgRNA1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK302.2

Plasmid

#235467

Purpose

Message phagemid carrying sgRNA2 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

sgRNA2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK302.3

Plasmid

#235468

Purpose

Message phagemid carrying sgRNA3 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

sgRNA3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK302.4

Plasmid

#235469

Purpose

Message phagemid carrying sgRNA4 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

sgRNA4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK302.5

Plasmid

#235470

Purpose

Message phagemid carrying sgRNA5 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

sgRNA5

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTaU6-esgRNA

Plasmid

#115630

Purpose

expression esgRNA in wheat protoplasts

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

TaU6p-esgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CBFRE (mt) EGFP

Plasmid

#26870

Depositor

Nicholas Gaiano

Article

Mizutani et al Nature. 2007 Sep 20. 449(7160):351-5.

Insert

CBFRE

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

The mutated CBF1 binding site: GATCTGGTGTAAA CAC…

Promoter

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pOsU3-esgRNA

Plasmid

#115629

Purpose

expression esgRNA in rice protoplasts

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

OsU3p-esgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDL127

Plasmid

#43911

Depositor

Stephen Elledge

Article

Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

Insert

DIF1 (DIF1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

HUG domain mutated VGMRIRQ->AAAAAAA, S59, Q66H

Promoter

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pCR3.1-OPNc M3

Plasmid

#106323

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

OPNc mutated splice site EEKQNAV-->AAAAAAA

Promoter

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pUC57-CmYLCV

Plasmid

#205245

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

CmYLCV

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLH-spsgRNA2

Plasmid

#64114

Purpose

Vector for expression of Sp sgRNA2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp sgRNA2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR8-attL3-pegRNA-attL2

Plasmid

#213052

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR8-attL1-pegRNA-attR3

Plasmid

#213051

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLH-nmsgRNA1.1

Plasmid

#64115

Purpose

Vector for expression of Nm sgRNA1.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm sgRNA1.1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR8-attL5-pegRNA-attR3

Plasmid

#213054

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR8-attR4-pegRNA-attR3

Plasmid

#213056

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR8-attL5-pegRNA-attL4

Plasmid

#213055

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR8-attL1-pegRNA-attR5

Plasmid

#213053

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-U6-AasgRNA

Plasmid

#121954

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
YN2_1_LT84_RPL13A

Plasmid

#177712

Purpose

Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

Cas9

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLY74

Plasmid

#130945

Purpose

Engineered sgRNA-G6 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-G6

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Plux2

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLY124

Plasmid

#130946

Purpose

Engineered sgRNA-G6-2MS2 (2 MS2 aptamers) generator circuit including promoter Pbad

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-G6-2MS2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pbad

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDNR-gRNA

Plasmid

#206990

Purpose

A plasmid for expression of SaCas9-sgRNA targeting donor plasmids

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Insert

Donor plasmid-targeting SaCas9-gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM201

Plasmid

#227660

Purpose

pRS305 3XFLAG-HRV3C-Rpn5-VTENKIF

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Use

Tags

3x-FLAG-HRV3C-

Expression

Yeast

Mutation

(amino acids 121-131) VTENKIF mutated to AAAAAAA

Promoter

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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