We narrowed to 1,220 results for: SUMO
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Plasmid#149529PurposeBacterial expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 13, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Pediculus humanus corporis PINK1 (143 - 575)
Plasmid#110751PurposeBacterial expression of PhPINK1 (crystallography)DepositorInsertPhum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
UseTagsN-His6-SUMOExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC
Plasmid#99649PurposeBacterial expression of wildtype Rag GTPase dimerDepositorUseTags10x His tag, Poly arginine linker, and SUMOExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(Q120L)
Plasmid#99701PurposeBacterial expression of RagA-RagC(Q120L)DepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationQ120LPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
eSrtA-Δ24-ASN
Plasmid#221849PurposeExpresses delta 24 alpha synuclein as 6XHis-SUMO-eSrtA-LPNTG-Δ24-ASN fusion proteinDepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionBacterialMutationNoPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY1935
Plasmid#212315PurposeExpress ApmFnuc protein with sumotagDepositorInsertIsvmimi
UseTags6xHis-thrombin site-TwinStrepTag-SUMOExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 AMSHLP 16-218
Plasmid#180609PurposeBacterial expression for AMSHLP MIT domain residues 16-218. Internal ID: WISP20-83DepositorInsertAMSHLP residues 16-218
UseTagsHIS-SUMOExpressionBacterialMutationResidues 16-218PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
SNAP-Kindlin
Plasmid#186136PurposeFor expression of recombinant Snap-labeled kindlin in bacteriaDepositorInsertFERMT2 (FERMT2 Human)
UseTagsHis6-sumoExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCm N-term MBP SMT3 NikJC199U
Plasmid#174363PurposeExpression plasmid with a ULP1 removable N-term MBP SUMO tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, MBP, SMT3, and TEVExpressionBacterialMutationC199UPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK RagA(R37P)-RagC
Plasmid#99706PurposeBacterial expression of RagA(R37P)-RagCDepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationR37PPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(L91P)
Plasmid#99707PurposeBacterial expression of RagA-RagC(L91P)DepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationL91PPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(K84T)
Plasmid#99708PurposeBacterial expression of RagA-RagC(K84T)DepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationK84TPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA(T21N)-RagC
Plasmid#99650PurposeBacterial expression of RagA(T21N)-RagCDepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationT21NPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(S75N)
Plasmid#99699PurposeBacterial expression of RagA-RagC(S75N)DepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationS75NPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA(Q66L)-RagC(Q120L)
Plasmid#99702PurposeBacterial expression of RagA(Q66L)-RagC(Q120L)DepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationQ120L and Q66LPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
PCV-Cas9
Plasmid#123643PurposeExpresses Cas9 with an N-terminal HUH-tag called PCV2DepositorInsertsUseTags(EAAAK)8 linker and His6 + SUMOExpressionBacterialMutation2-116PromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-hUba2-His
Plasmid#53117Purposeexpression of full length human Uba2 with Cterminal His-tag in E.coliDepositorInsertUBA2 (UBA2 Human)
UseTagsHis and T7 tagExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOPINS-AREL1
Plasmid#66710PurposeExpresses AREL1 HECT domain in E. coliDepositorInsertKIAA0317 (AREL1 )
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cas9-PCV
Plasmid#123644PurposeExpresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coliDepositorInsertsUseTags6xHis and SUMOExpressionBacterialMutation2-116PromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136458
Plasmid#149531PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 13, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
WDV
Plasmid#226983PurposeExpresses the HUH endonuclease WDV, used as a no ligand control for DNA tension sensor experimentsDepositorInsertWDV (repA )
UseTags6xHis and SUMOExpressionBacterialMutationPromoterT7Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528-PPxY1-GS-WW3e
Plasmid#166705Purposeexpresses an N-terminal fusion of AMOT PPxY1 to NEDD4L WW3 in bacterial cellsDepositorInsertAMOT PPxY1 fused to NEDD4L WW3
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9
Plasmid#133909PurposeBacterial expression of His6-Ubc9DepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMIIS793
Plasmid#238206PurposeExpresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanRDepositorInsertTaTasR
UseTagsTwinStrepII-bdSUMOExpressionBacterialMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
HIS-HA-CMYC P53
Plasmid#233738Purposeexpresses HIS-HA-CMYC triple tagged P53 for pulldown assayDepositorInsertHIS-HA-CMYC-p53 (TP53 Human)
UseTagsHIS-HA-CMYCExpressionBacterialMutationPromoterT7Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIA1385
Plasmid#166862PurposeCodon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.DepositorInsertSARS-COV-2 Nsp12 (ORF1ab Synthetic)
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCA528-AMOT(1-300)-sortase
Plasmid#166706Purposeexpresses AMOT(1-300) with C-terminal sortase tag in bacterial cellsDepositorInsertAMOT aa 1-300
UseTagsHis-SUMO and Sortase tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINS-USP21 (USP, aa 196-565)
Plasmid#61585PurposeExpresses human USP21 (USP domain) in E. coli.DepositorInsertUSP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)
UseTagsHis6-SUMOExpressionBacterialMutationDeleted aa 1-195.PromoterT7Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
RBD#4L
Plasmid#193021PurposehnRNP C RRM RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13aDepositorInsertCas13a
UseTagshnRNP C RRMExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
RBD#3L
Plasmid#193020PurposehnRNP A1 RRM2 RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13aDepositorInsertCas13a
UseTagshnRNP A1 RRM2ExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2
Plasmid#166972PurposeExpresses FBF-2 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
UseTagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575PromoterT7Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9-C93A
Plasmid#133910PurposeBacterial expression of His6-Ubc9-C93ADepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationCysteine 93 to AlaninePromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9-C138A
Plasmid#133911PurposeBacterial expression of His6-Ubc9-C138ADepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationCysteine 138 to AlaninePromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1 (1-366)
Plasmid#193039PurposeBacterial expression plasmid for full-length IST1 (1-366).DepositorInsertIST1 (IST1 Human)
UseTags6xHis-SumoExpressionBacterialMutationPromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CAPN7 (1-75)
Plasmid#193031PurposeBacterial expression plasmid for CAPN7 (1-75) N-terminal MIT domainDepositorInsertCAPN7 (CAPN7 Human)
UseTags6xHis-SumoExpressionBacterialMutationResidues 1-75PromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CAPN7 (81-165)
Plasmid#193032PurposeBacterial expression plasmid for CAPN7 (81-165) C-terminal MIT domainDepositorInsertCAPN7 (CAPN7 Human)
UseTags6xHis-SumoExpressionBacterialMutationResidues 81-165PromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP7 366-453
Plasmid#104598PurposeExpresses C-terminal 366-453 residues of CHMP7 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-12DepositorInsertCHMP7 residues 366-453 (CHMP7 Human)
UseTagsHis-SUMOExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTVKRSF225
Plasmid#135005PurposeMusF2 prenyltransferases from Nmu. PCC 7906DepositorInsertMusF2 prenyltransferaseM
UseTagsSUMO fusionExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBHRSF227
Plasmid#135006PurposeMusF1 prenyltransferase from Nostoc sp. UHCC 0398DepositorInsertMusF1 prenyltransferase
UseTagsSUMO fusionExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
WDV-Echistatin
Plasmid#226985PurposeExpresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensorsDepositorInsertsUseTags6xHis and SUMOExpressionBacterialMutationPromoterT7Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDF-rM1PYK
Plasmid#220232PurposeInducible expression of rabbit muscle pyruvate kinase isoform 1DepositorInsertRabbit muscle pyruvate kinase (PKM )
UseTags6xHis-MBP-SUMOExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHTSHP-Smt-IIShhN
Plasmid#121135PurposeExpresses N-terminal SUMO tagged II-ShhN in bacteriaDepositorInsertShh (Shh Mouse)
UseTagsHIS, HRV3C site, MBP, and Smt tagExpressionBacterialMutationAmino acid sequence from 26-195, with two extra i…PromotertacAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCA528 huPQBP1
Plasmid#217449PurposeExpresses full-length human PQBP1 from a His-SUMO bacterial expression vectorDepositorInsertPQBP1 (PQBP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1 (322-366)
Plasmid#193038PurposeBacterial expression plasmid for IST1 (322-366), the minimal construct with full binding to CAPN7 MIT domains.DepositorInsertIST1 (IST1 Human)
UseTags6xHis-SumoExpressionBacterialMutationResidues 322-366PromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1 L328D, L355A (1-366)
Plasmid#193040PurposeBacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.DepositorInsertIST1 (IST1 Human)
UseTags6xHis-SumoExpressionBacterialMutationL328D, L355APromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CAPN7 L61D (1-165)
Plasmid#193033PurposeBacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutationDepositorInsertCAPN7 (CAPN7 Human)
UseTags6xHis-SumoExpressionBacterialMutationL61D, Residues 1-165PromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CAPN7 V18D,F98D (1-165)
Plasmid#193034PurposeBacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.DepositorInsertCAPN7 (CAPN7 Human)
UseTags6xHis-SumoExpressionBacterialMutationV18D, F98D, Residues 1-165PromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1, residues 316-366, C-Cys
Plasmid#191234PurposeBacterial expression for IST1 residues 316-366, contains non-native C-terminal Cysteine for labelingDepositorInsertIST1 (IST1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 316-366, contains nonnative C-terminus f…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP4A 153-222
Plasmid#108256PurposeExpresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6DepositorInsertCHMP4A residues 153-222 (CHMP4A Human)
UseTagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only