Addgene

Showing: 341 - 360 of 3512 results

Results per page:

Sort results by:

OpHA-delta C6-Y98A

Plasmid

#127685

Purpose

Bacterial expression of His-tagged OpHA-delta C6-Y98A

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OpHA-delta C6-Y98A

Use

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y98A

Promoter

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
OphA-delta C6-Y98F

Plasmid

#127686

Purpose

Bacterial expression of His-tagged OphA-delta C6-Y98F

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Y98F

Use

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y98F

Promoter

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
OphA-delta C6-Q172A

Plasmid

#127687

Purpose

Bacterial expression of His-tagged OphA-delta C6-Q172A

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Q172A

Use

Tags

His8

Expression

Bacterial

Mutation

delta C6-Q172A

Promoter

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
OphA-delta C6-W400A

Plasmid

#127688

Purpose

Bacterial expression of His-tagged OphA-delta C6-W400A

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-W400A

Use

Tags

His8

Expression

Bacterial

Mutation

delta C6-W400A

Promoter

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
OphA-delta C6-P399A

Plasmid

#127689

Purpose

Bacterial expression of His-tagged OphA-delta C6-P399A

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-P399A

Use

Tags

His8

Expression

Bacterial

Mutation

delta C6-P399A

Promoter

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pClodANL-FGF4

Plasmid

#107874

Purpose

Bacterial expression of FGF4-NanoLuc

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

FGF4 (FGF4 Synthetic)

Use

Luciferase

Tags

NanoLuc

Expression

Bacterial

Mutation

Promoter

pro D

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC24

Plasmid

#104798

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre2). Also expresses Cas9 from rolD promoter from AtUBQ10 promoter

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g101120, Medtr2g101130

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-F-del Casp9.IRES.GFP

Plasmid

#15567

Depositor

David Spencer

Article

Straathof et al Blood. 2005 Jun 1. 105(11):4247-54.

Insert

FKBP12(V36)-p30Caspase9 (FKBP1A Human)

Use

Retroviral

Tags

FKBP12 and HA epitope

Expression

Mammalian

Mutation

FKBP12 F36 to V Truncated Caspase9 (135-416; lac…

Promoter

Available since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pZac2.1 U6-SaDMDR7-U6-SaDMDL2

Plasmid

#78603

Purpose

AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

U6-SaDMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only
plenti2-EF1a-ChR2-EYFP-WRPE

Plasmid

#125255

Purpose

3rd gen lentiviral expression of ChR2 with mutation fused to EYFP driven by EF1a promoter for optogenetic activation

Depositor

Roger D. Kamm

Article

Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

Insert

hChR2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo

Plasmid

#191397

Purpose

sgRNA targeting alpha-satellites on human chromosome 9

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

sgRNA targeting alpha satellites on human chromosome 9

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
sg1+sg2+sg3

Plasmid

#113969

Purpose

Triple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg1+sg2+sg3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSirenRetroQ(puro)-shEgr3

Plasmid

#108115

Purpose

pSIREN-RetroQ vector containing shRNA sequence to Egr3

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

shRNA to Egr3 (Egr3 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR/U6 HDAC4 shRNA

Plasmid

#32220

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

shRNA against murine HDAC4 (Hdac4 Mouse)

Use

RNAi; Gateway u6 entry vector

Tags

Expression

Mutation

Promoter

human U6

Available since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only
pY71-PT7-RiboJ-sfGFP-MGapt

Plasmid

#129119

Purpose

Expresses a fluorescent reporter for the quantification of cell-free protein expression.

Depositor

Matthew Lux

Article

Cole et al ACS Synth Biol. 2019 Sep 20;8(9):2080-20

Insert

Superfolder GFP with Malachite Green aptamer

Use

Synthetic Biology

Tags

His6 and RiboJ Insulator

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-Topo U6-SpAi9L-U6SpDMDL

Plasmid

#78608

Purpose

Expresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

U6-SpAi9L-U6SpDMDL

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only
esgRNA_NbATML1-2pro and NbATML1-1pro

Plasmid

#231155

Purpose

T-DNA encoding TRV2 with mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro

Use

Tags

Expression

Plant

Mutation

Promoter

PEBV sub-genomic

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3Luc-5XNF-kappaB

Plasmid

#185695

Purpose

Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

Depositor

Esther López-Bayghen

Article

Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

Insert

NF-kappaB (NFKB1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

NF-kappaB binding site

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES2-EGFP-p53 WT

Plasmid

#49242

Purpose

expresses human wt p53 and EGFP in mammalian cells

Depositor

Dylan Taatjes

Article

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53 (TP53 Human)

Use

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-CIB1-Myc

Plasmid

#32505

Depositor

John Groffen, Nora Heisterkamp

Article

Haataja et al J Biol Chem. 2002 Mar 8;277(10):8321-8.

Insert

CIB1 (CIB1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only