-
Plasmid#61576PurposeRecombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locusDepositorInsertchromatin insulator flanked TRE-LSL-tdTomato
UseRecombinase-mediated cassette exchange using flp …TagsExpressionMutationPromoterAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-POMP-PSMG1-PSMG2-PSMG3-PSMG4
Plasmid#214137PurposeInsect cell expression of the human 20SCP proteasome assembly chaperonesDepositorInsertsUseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC003 - LshC2C2 locus into pACYC184 for spacer cloning
Plasmid#79152PurposeLshC2c2 locus in pACYC184 with BsaI sites for spacer cloningDepositorInsertLshC2c2 locus
UseTagsExpressionMutation*PromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNAz-mBACEflag (C474A/C478A/C482A/C485A)
Plasmid#26736DepositorInsertBACE (Bace1 Mouse)
UseTagsFLAGExpressionMammalianMutationCys 474, 478, 482, and 485 mutated to AlaPromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII
Plasmid#217896PurposeInsect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tagDepositorUseTags2xSTIIExpressionInsectMutationPromoterAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo
Plasmid#191397PurposesgRNA targeting alpha-satellites on human chromosome 9DepositorInsertsgRNA targeting alpha satellites on human chromosome 9
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (CCR5 TALEN Sce Spacer)
Plasmid#46941PurposeCodes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with TALEN TS
+3 mCherry
3' iRFP arm
UseLentiviralTagsExpressionBacterial and MammalianMutationembedded TALEN TS from 163-218, truncated 25 amin…PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-mCerulean3-spacer-cpVenus[E172]
Plasmid#125204PurposeSB-transposon plasmid for stable expression of mCerulean3-cpVenus[E172] FRET low controlDepositorInsertmCerulean3-spacer-cpVenus[E172]
UseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC022 - LbnCas13a from Lachnospiraceae bacterium NK4A179
Plasmid#91912PurposeExpresses LbnCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLbnCas13a and guide expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC021 - LbmCas13a from Lachnospiraceae bacterium MA2020
Plasmid#91911PurposeExpresses LbmCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLbmCas13a and guide expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1_Strep-Psc-BICD2(711-800)_L786A
Plasmid#228838PurposeInsect cell expression of the mutant C-terminus of BICD2DepositorInsertBICD2 (Bicd2 Mouse)
UseTagsStrepExpressionInsectMutationaa 711-800, with L786APromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1_Strep-Psc-BICD2(711-800)_L742A
Plasmid#228839PurposeInsect cell expression of the mutant C-terminus of BICD2DepositorInsertBICD2 (Bicd2 Mouse)
UseTagsStrepExpressionInsectMutationaa 711-800, with L742APromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2[R.ferrum]-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR
Plasmid#190078Purposeexpress Rhinolophus ferrumequinum ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance geneDepositorInsertACE2 (TMPRSS2 Rhinolophus ferrumequinum)
UseTagsExpressionMammalianMutationPromoternoneAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-gamma-tubulin(N229A)-TEV-His6
Plasmid#178077PurposeInsect cell expression of gamma-tubulin(N229A)-TEV-His6DepositorInsertgamma-tubulin with an N229A point mutation (TUBG1 Human)
UseTagsTEV-His6ExpressionInsectMutationChanged Asparagine 229 to AlaninePromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Ac-AChBP
Plasmid#63128PurposeExpression of Aplysia californica AChBPDepositorInsertAChBP
UseTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTBL402 AC sgRNA
Plasmid#126427PurposeTo target a protospacer with AC at the cut site.DepositorInsertspacer against human genome with AC at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635
Plasmid#91918PurposeExpresses LbfCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLbfCas13a and guide expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA
Plasmid#197026PurposeVector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoterDepositorInserthumanized enOsCas12f1
UseTagsExpressionMammalianMutationD52R / T132RPromoterCBh, CMV, hU6Available sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA
Plasmid#197027PurposeVector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoterDepositorInserthumanized enRhCas12f1
UseTagsExpressionMammalianMutationL270RPromoterCBh, CMV, hU6Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2[dEcto]_IRES-mCherry-H2A-P2A-PuroR
Plasmid#171596PurposeEctodomain deleted negative control ACE2 from Bxb1 landing padDepositorInsertsUseSynthetic Biology; Promoterless bxb1 dna recombin…TagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only