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pLEGFP-Y705F-STAT3

Plasmid

#71445

Purpose

Retroviral expression of Y705F-STAT3. Please note that this plasmid contains Y705F-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Mutation

Y705F

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pOTTC946 - pAAV CMV-IE NK1R-IRES-EGFP

Plasmid

#102935

Purpose

An AAV vector that expresses rat NK1R-IRES-EGFP under the CMV-IE promoter

Depositor

Brandon Harvey

Article

Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

Insert

NK1R (Tacr1 Rat)

Use

AAV

Expression

Mammalian

Promoter

CMV-IE

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC385 - pAAV CMV-IE IRES EGFP

Plasmid

#102936

Purpose

An AAV vector that expresses IRES EGFP under the CMV-IE promoter

Depositor

Brandon Harvey

Article

Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

CMV-IE

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGST2-GST-TEV-NDP52

Plasmid

#187828

Purpose

Plasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NDP52 (CALCOCO2 Human)

Tags

GST-TEV

Expression

Bacterial

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX Stat3-SH2

Plasmid

#46513

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat3 (STAT3 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 584-700

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pMX-IY HA-GBRPL2

Plasmid

#89298

Purpose

For expression of the mammalian Atg8 protein GABARAPL2 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Retroviral

Tags

HA and IRES-YFP

Expression

Mammalian

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMX-IY HA-GBRPL1

Plasmid

#89297

Purpose

For expression of the mammalian Atg8 protein GABARAPL1 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAPL1 (GABARAPL1 Human)

Use

Retroviral

Tags

HA and IRES-YFP

Expression

Mammalian

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMX-IY GBRPL1

Plasmid

#89294

Purpose

For expression of the mammalian Atg8 protein GABARAPL1 without a tag

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAPL1 (GABARAPL1 Human)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMX-IY LC3A

Plasmid

#89290

Purpose

For expression of the mammalian Atg8 protein LC3A without a tag

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

LC3A (MAP1LC3A Human)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF1-MCS2-EF1-Puro-RGS7

Plasmid

#113728

Purpose

Express RGS7 in mamalian cells

Depositor

Yardena Samuels

Article

Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

Insert

RGS7 (RGS7 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMX-IY LC3B

Plasmid

#89291

Purpose

For expression of the mammalian Atg8 protein LC3B without a tag

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

LC3B (MAP1LC3B Human)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMX-IY GBRPL2

Plasmid

#89295

Purpose

For expression of the mammalian Atg8 protein GABARAPL2 without a tag

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGG-GST-TEV-mCherry-NAP1 (WT)

Plasmid

#198036

Purpose

Used for the expression and purification of mCherry NAP1 in a mammalian expression system (SMC Internal No. 2065)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NAP1 (AZI2 Human)

Tags

GST-TEV and mCherry

Expression

Mammalian

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGG-GST-TEV-NAP1 (WT)

Plasmid

#198034

Purpose

Used for the expression and purification of NAP1 in a mammalian expression system (SMC Internal No. 2064)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NAP1 (AZI2 Human)

Tags

GST-TEV

Expression

Mammalian

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)

Plasmid

#198072

Purpose

Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Tags

GST-TEV-EGFP

Expression

Insect

Mutation

TBK1ΔCTD (res 1-666)

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pGST2-GST-TEV-OPTN S177D S473D

Plasmid

#187827

Purpose

Plasmid for the expression and purification of GST-TEV-OPTN S177D S473D. Internal Ref: SMC1588

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

OPTN (OPTN Human)

Tags

GST-TEV

Expression

Bacterial

Mutation

S177D S473D

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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EF.STAT3C.Ubc.GFP

Plasmid

#24983

Purpose

3rd generation lentiviral expression of constitutively active Stat3

Depositor

Linzhao Cheng

Article

Hillion et al Cancer Research 68, 10121, December 2008

Insert

STAT3 (STAT3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

A662C, N664C (constitutively active)

Available Since

May 28, 2010

Availability

Academic Institutions and Nonprofits only

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pmCherry-N1 GBP (GBP-mCherry)

Plasmid

#162879

Purpose

Expression in mammalian cells of GFP binding protein (GBP) tagged with mCherry

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

GFP Binding Protein

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)

Plasmid

#198035

Purpose

Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

SINTBAD (TBKBP1 Human)

Tags

EGFP and GST-TEV

Expression

Insect

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pBT272_(pRosa26-GTET)

Plasmid

#36882

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
diphteria toxin A

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

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