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pDEST47-Arl13b12

Plasmid

#40872

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

no stop codon

Promoter

CMV

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL18

Plasmid

#183390

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

pro-IL18 (IL18 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNeonGreen-LEHD-NanoLuc

Plasmid

#98289

Purpose

caspase-9 BRET Sensor

Depositor

Maarten Merkx

Article

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Use

Tags

His-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST40-Arl13b11

Plasmid

#40873

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Tags

His and V5

Expression

Mammalian

Mutation

no stop codon

Promoter

CMV

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
pDEST40-Arl13b89

Plasmid

#40874

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-Arl13b2

Plasmid

#40871

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Gateway entry

Tags

Expression

Mutation

no stop codon

Promoter

Available since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only
FL MALT1 WT in pET29b-Strep

Plasmid

#48968

Purpose

Expresses Full length wild type MALT1 in e.coli

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 isoform A (MALT1 Human)

Use

Tags

His and Strep

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
pDEST47-Arl13b17

Plasmid

#40875

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains Arl13b amino acids 1-199

Promoter

CMV

Available since

March 13, 2013

Availability

Academic Institutions and Nonprofits only
pET21 Catalytic Domain of MALT1

Plasmid

#48970

Purpose

Expresses catalytic domain of MALT1 in e.coli AA 329-566

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 (MALT1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains AA 329-566

Promoter

T7

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
pDEST53-Arl13b20

Plasmid

#40876

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted Arl N-terminal domain; added stop codon

Promoter

CMV

Available since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only
TAL3039

Plasmid

#41213

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-casp8ap2-Right (casp8ap2 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 1, 2013

Availability

Academic Institutions and Nonprofits only
TAL3038

Plasmid

#41212

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-casp8ap2-Left (casp8ap2 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pmAmetrine-DEVD-tdTomato

Plasmid

#18879

Purpose

Caspase-3 FRET biosensor, mammalian expression of mAmetrine-DEVD-tdTomato

Depositor

Robert Campbell

Article

Ai et al Nat Methods. 2008 May . 5(5):401-3.

Insert

mAmetrine-DEVD-tdTomato (CASP3 lab constructed, Human)

Use

Tags

Expression

Mammalian

Mutation

mAmetrine-tdTomato linker sequence: ...ITLGGTGSGS…

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP/golgin-160/3DE

Plasmid

#180631

Purpose

Expression of caspase-resistant golgin-160

Depositor

Carolyn Machamer

Article

Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

Insert

golgin-160/3DE (GOLGA3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D59E, D139E, D311E

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FL MALT1 Catalytic Mutant in pET29b-Strep

Plasmid

#48969

Purpose

Expresses Full length catalytic mutant MALT1 in e.coli

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 isoform A (MALT1 Human)

Use

Tags

His and Strep

Expression

Bacterial

Mutation

Catalytic Mutant C464A

Promoter

T7

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
pET21 Catalytic Domain of MALT1 Catalytic Mutant

Plasmid

#48971

Purpose

Expresses catalytic domain of MALT1 catalytic mutant in e.coli AA 329-566

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 (MALT1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains AA 329-566; Catalytic Mutant C464A

Promoter

T7

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
pT2iApowriter2(#336)

Plasmid

#184104

Purpose

cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic

Depositor

Sophie Vriz

Article

Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

Insert

myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2

Use

Zebrafish transient transgenesis

Tags

internal tag: 5myc

Expression

Mutation

DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT22Apowriter2(#340)

Plasmid

#184105

Purpose

cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

Insert

myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2

Use

Zebrafish transgenesis (blue eyes)

Tags

internal tag: 5myc

Expression

Mutation

DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT22iuasvApoWtEos3N(#486)

Plasmid

#184107

Purpose

cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

Insert

myr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2

Use

Zebrafish transgenesis (blue eyes)

Tags

Expression

Mutation

DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

Promoter

4xUAS

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT22iuasvApoWChe(#488)

Plasmid

#184108

Purpose

cyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

Insert

myr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2

Use

Zebrafish transgenesis (blue eyes)

Tags

Expression

Mutation

DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

Promoter

4xUAS

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge