We narrowed to 389 results for: Tight TRE

1. pLenti-nSpCas9-TadA8e

   Plasmid

   #209044

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TadA8e-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-nSpCas9-TadA8.20m

   Plasmid

   #209045

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8.20m is fused to the N-terminus of SpCas9(D10A)-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TadA8.20m-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-nSpCas9-APOBEC3A

   Plasmid

   #209040

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   APOBEC3A-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-nSpCas9-NoCBE

   Plasmid

   #209038

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase) linked to ssDNA binding domain from RAD51 and uracil DNA glycosylase inhibitor (UGI)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-nSpCas9-APOBEC1

   Plasmid

   #209039

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   APOBEC1-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-nSpCas9-BEACON2

   Plasmid

   #209041

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   BEACON2-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-nSpCas9-evoCDA1

   Plasmid

   #209042

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   evoCDA1-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUt-mPF-GFP

   Plasmid

   #199222

   Purpose

   LPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Insert

   rtTA-Advanced::P2A::EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRER-PhoCl-Insert

   Plasmid

   #219675

   Purpose

   Caging at lumenal N-ter of POI: TMEM-PhoCl-MCS in pTREtight2

   Depositor

   Helge Ewers

   Article

   Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

   Insert

   RER-PhoCl
   

   Use

   Tags

   PhoCl

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   June 14, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pTSA-38

    Plasmid

    #17328

    Depositor

    Takeshi Sano

    Article

    Sano et al Proc Natl Acad Sci U S A. 1995 Apr 11. 9

    Insert

    streptavidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Truncated to include only amino acids 16-133 of m…

    Promoter

    Available since

    Feb. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pInsert-PhoCl-RER

    Plasmid

    #219676

    Purpose

    Caging at cytosolic C-ter of POI: mScarletCD4-PhoCl-RER in pTREtight2

    Depositor

    Helge Ewers

    Article

    Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

    Insert

    CD4mScarlet-PhoCl-RER
    

    Use

    Tags

    mScarlet and PhoCl

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. aCE89

    Plasmid

    #204494

    Purpose

    TREtight promoted WBeta and PhlF promoted PhiC31 without PEST tags

    Depositor

    Ron Weiss

    Article

    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

    Inserts

    φC31 integrase
    WBeta recombinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    PhIF and TREtight

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. aCE90

    Plasmid

    #204495

    Purpose

    TREtight promoted WBeta and PhlF promoted PhiC31 with PEST tags

    Depositor

    Ron Weiss

    Article

    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

    Inserts

    phiC31 recombinase
    WBeta recombinase
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    PhIF and TREtight

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TtGNN-TAF4-HFD

    Plasmid

    #106381

    Purpose

    dox inducible expression of GFP fused TAF4 HFD (827-909) with neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    GFP fused TAF4 HFD (827-909) (Taf4 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    TREtight

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLVX-UbC-rtTA-Ngn2:2A:EGFP

    Plasmid

    #127288

    Purpose

    all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

    Depositor

    Fred Gage

    Article

    Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

    Insert

    Ngn2:2A:Ascl1 (NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Ngn2 and EGFP linked via 2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

    Plasmid

    #132389

    Purpose

    Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    human TauWT 2N4R-EGFP (MAPT Synthetic, Human)
    

    Use

    CRISPR and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    TREtight

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

    Plasmid

    #132393

    Purpose

    Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    human TauP301L 2N4R-EGFP (MAPT Synthetic, Human)
    

    Use

    CRISPR and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Proline 301 to Leucine (P301L)

    Promoter

    TREtight

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCHW101020

    Plasmid

    #222454

    Purpose

    Level 1, Position 2 containing 35S::CUP2-p65::NOS.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    35S::CUP2-p65::NOS (CUP2 )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCHW00011

    Plasmid

    #222439

    Purpose

    Level 0, CDSns module (AATG-TTCG) containing S. cerevisiae CUP2.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    CUP2 (Saccharomyces cerevisiae) (CUP2 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28b-AF0623

    Plasmid

    #30164

    Depositor

    SeWon Suh

    Article

    Kim do et al Acta Crystallogr Sect F Struct Biol Crys

    Insert

    AF0623 (lig Archaeoglobus fulgidus)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only