Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,065 results for: control gRNA

Clear keywords
Showing: 341 - 360 of 1065 results

  2. pSB700-mouse-ACTC1-Puro

    Plasmid
    #128326
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against mouse ACTC1 for activation (Actc1 Mouse)
    Expression
    Mammalian
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128326-plasmid-map-sequence-id-255029

    Enlarge

  5. pHEE401

    Plasmid
    #71286
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…
    Available Since
    Jan. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    71286-plasmid-map-sequence-id-127777

    Enlarge

  9. pNICKclos2.0

    Plasmid
    #73228
    Purpose
    Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052
    Depositor
    Sheng Yang
    Article
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Inserts
    Cas9 nickase
    sgRNA to xylR
    Use
    E.coli - clostridium shuttle vector
    Mutation
    D10A
    Promoter
    Pj23119 and Pthl
    Available Since
    June 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    73228-plasmid-map-sequence-id-178086

    Enlarge

  12. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid
    #127668
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters
    Depositor
    Jennifer Phillips-Cremins
    Article
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Insert
    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a and hU6
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127668-plasmid-map-sequence-id-247046

    Enlarge

  14. pdCASclos

    Plasmid
    #73640
    Purpose
    Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824
    Depositor
    Sheng Yang
    Article
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Inserts
    dCas9
    sgRNA to spo0A
    Use
    E.coli-clostridium shuttle vector
    Mutation
    D10A, H840A
    Promoter
    Pj23119 and ptb
    Available Since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73640-plasmid-map-sequence-id-148604

    Enlarge

  15. pdCas9-DNMT3A-PuroR_hNT

    Plasmid
    #71830
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    Non-targeting sgRNA human (DNMT3A Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71830-plasmid-map-sequence-id-131025

    Enlarge

  16. ipUSEPR-sg-Hs-PHGDH-int2

    Plasmid
    #188682
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPHGDH intron control (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Mutation
    WT
    Available Since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188682-plasmid-map-sequence-id-375035

    Enlarge

  17. ipUSEPR-sg-Hs-PHGDH-int1

    Plasmid
    #188681
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPHGDH intron control (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Mutation
    WT
    Available Since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188681-plasmid-map-sequence-id-375018

    Enlarge

  18. ipUSEPR-sg-Hs-PHGDH-int3

    Plasmid
    #188683
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPHGDH intron control (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Mutation
    WT
    Available Since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188683-plasmid-map-sequence-id-375016

    Enlarge

Showing: 341 - 360 of 1065 results