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pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI

Plasmid

#115219

Purpose

Expresses PspCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PspCas13

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 21, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI

Plasmid

#115209

Purpose

Expresses PbuCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PbuCas13

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI

Plasmid

#115216

Purpose

Expresses Pin2Cas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

Pin2Cas13

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Plasmid

#58440

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Use

Tags

Expression

Yeast

Mutation

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI

Plasmid

#115208

Purpose

Expresses PinCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PinCas13

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Plasmid

#58439

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Use

Tags

Expression

Yeast

Mutation

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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p255 pPr-CREM/Ins/CMV-STOP-luc

Plasmid

#8408

Purpose

Modified cre expressed from probasin, separated from the Cre-dependent CMV-STOP-luc cassette by insulators.

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CREM/insulators/lox/luciferase

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

modified Cre

Promoter

Probasin, CMV

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI

Plasmid

#115207

Purpose

Expresses BzoCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

BzoCas13

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Plasmid

#58441

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 3 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Use

Tags

Expression

Yeast

Mutation

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-Gαi3-YFP (Citrine variant, b/c loop insert)

Plasmid

#171375

Purpose

For the mammalian expression of rat Gαi3 internally tagged with YFP

Depositor

Mikel Garcia-Marcos

Article

Marivin et al Sci Signal. 2016 Apr 12;9(423):ra37. doi

Insert

Gai3-YFP

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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M59 pPET28a-TEV-human beta cat domains R1-C

Plasmid

#17200

Depositor

Randall Moon

Insert

beta catenin (CTNNB1 Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

domains R1C

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172504

Purpose

Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI

Plasmid

#115220

Purpose

Expresses Pin3Cas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

Pin3Cas13

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

Plasmid

#115210

Purpose

Expresses AspCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

AspCas13

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI

Plasmid

#115214

Purpose

Expresses PauCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PauCas13

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

Plasmid

#229819

Purpose

A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Type

Empty backbone

Use

Tol2 destination vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 InsertionR214-EPE

Plasmid

#212461

Purpose

Encodes SARS-CoV-2 spike R214 insertion for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike R214 insertion (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

R214 insertion

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV puro PRDM14 ΔSET+ΔZinc finger domains

Plasmid

#193678

Purpose

Constitutive retroviral expression of PRDM14

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Retroviral

Tags

Expression

Mutation

Deletion of SET and Zinc finger domains

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

Plasmid

#91819

Purpose

Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Use

Tags

10xHis

Expression

Yeast

Mutation

5xGly linker, PreScission Protease site (LEVLFQGP…

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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M61 pPET28c-Xenopus beta catenin domains R10C

Plasmid

#17202

Depositor

Randall Moon

Insert

beta catenin (ctnnb1.L Frog)

Use

Tags

HIS

Expression

Bacterial

Mutation

domains R10C

Promoter

Available since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only