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PX458_CIC_iso1_1

Plasmid

#135743

Purpose

Encodes gRNA for 3' target of human CIC_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CIC_iso1 gRNA (CIC Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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deltaMyc-HA

Plasmid

#16556

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC (MYC Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of aa 40-178

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
HOXC8-pSG5

Plasmid

#21001

Depositor

Corey Largman

Article

Lawrence et al Cell Growth Differ. 1993 Aug . 4(8):665-

Insert

HOXC8 (HOXC8 Human)

Expression

Mammalian

Mutation

missing first 24 N-terminal amino acids of coding…

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pGFP-PTEN D92A/C124A

Plasmid

#50522

Depositor

Kenneth Yamada

Article

Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

Insert

PTEN (PTEN Human)

Tags

GFP

Expression

Mammalian

Mutation

D92A/C124A

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pGST2-CeEP55

Plasmid

#21501

Depositor

James Hurley

Article

Lee et al Science. 2008 Oct 24. 322(5901):576-80.

Insert

CEP55-EABR (ESCRT and ALIX Binding Region) domain (CEP55 Human)

Tags

GST

Expression

Bacterial

Mutation

Q230 (CAG changed to TAA), Q218 (CAA changed to T…

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only
pAc-His-zfTpo

Plasmid

#64309

Purpose

Express soluble His-tagged zebrafish thrombopoietin in insect cells

Depositor

Petr Bartunek

Article

Svoboda et al Blood. 2014 Jul 10;124(2):220-8. doi: 10

Insert

Thrombopoietin (thpo Zebrafish)

Tags

(His)6

Expression

Insect

Mutation

Thrombopoietin starts at AA37 (AA1-36 encode the …

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only
pCS2-zfKitlgb

Plasmid

#168200

Purpose

Express zebrafish Kitlgb, mRNA synthesis

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlgb (kitlgb Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Mutation

I85V, V222I, and D233N- please see depositor comm…

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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NLS-dCas9-HA-NLS-GFP4-VPR

Plasmid

#183924

Purpose

VPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

dCas9-GFP4-VPR

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-E3_5-HilA

Plasmid

#153326

Purpose

Triggers secretion of SptP120 fused to the E3_5 DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-E3_5 DARPin

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-K27-HilA

Plasmid

#153328

Purpose

Triggers secretion of SptP120 fused to the K27 DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-K27 DARPin

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_YFP

Plasmid

#45278

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

YFP

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pQCXIH-Flag Smad3 EPSM

Plasmid

#27026

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

T179V S204A S208A S213A

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pBabe_puro_DEST_Flag_SAMD7

Plasmid

#45275

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

SAMD7 (SAMD7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-WW-CR-CT

Plasmid

#18069

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Tags

GST

Expression

Bacterial

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-2T SHP1 deltaP

Plasmid

#8596

Depositor

Ben Neel

Article

Timms et al Mol Cell Biol 1998 Jul;18(7):3838-50.

Insert

SHP1 (PTPN6 Human)

Tags

GST

Expression

Bacterial

Mutation

deltaP (deletion of phosphatase domain, amino aci…

Available Since

June 21, 2005

Availability

Academic Institutions and Nonprofits only
pFETCh_MBD1_iso1

Plasmid

#86263

Purpose

Donor vector for 3' FLAG tag of human MBD1_iso1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MBD1_iso1 homology arms (MBD1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pTH33 [pmyo-3::YFP-BeCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168169

Purpose

Expression of YFP-BeCyclOp(A-2x) in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)

Tags

YFP

Expression

Worm

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMS28: pTns(PmcCAST)_ΔTniQ,ΔTnsD

Plasmid

#168161

Purpose

Inducible expression of PmcCAST TnsAB and TnsC proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

PmcCAST Tns proteins (TnsAB and TnsC)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS26: pTns(PmcCAST)_ΔTniQ

Plasmid

#168159

Purpose

Inducible expression of PmcCAST TnsAB, TnsC and TnsD proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

PmcCAST Tns proteins (TnsAB, TnsC and TnsD)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS27: pTns(PmcCAST)_ΔTnsD

Plasmid

#168160

Purpose

Inducible expression of PmcCAST TnsAB, TnsC and TniQ proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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