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We narrowed to 15,985 results for: nes

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Showing: 3581 - 3600 of 15985 results

  2. SpCas9-HF1-plus

    Plasmid
    #126768
    Purpose
    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126768-plasmid-map-sequence-id-410966

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  3. pSpG-PPE

    Plasmid
    #170130
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103
    Insert
    nCas9-SpG(H840A)-M-MLV
    Use
    CRISPR
    Expression
    Plant
    Mutation
    H840A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1…
    Promoter
    maize Ubiquitin-1
    Available Since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170130-plasmid-map-sequence-id-333605

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  6. pVenus-N1 (804)

    Plasmid
    #61854
    Purpose
    empty backbone for tagging proteins at the C-terminus with Venus
    Depositor
    Jonathon Pines
    Type
    Empty backbone
    Tags
    Venus
    Expression
    Mammalian
    Available Since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61854-plasmid-map-sequence-id-375257

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  7. LentiCRISPRv2 RFP670

    Plasmid
    #187646
    Purpose
    lentiviral vector expressing RFP670 alongside Cas9 and an sgRNA cloning site
    Depositor
    Martin Carroll
    Insert
    RFP 670
    Use
    Lentiviral
    Tags
    RFP670 and sgRNA cloning site
    Promoter
    EFS (P2A)
    Available Since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187646-plasmid-map-sequence-id-367454

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  10. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196078
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196078-plasmid-map-sequence-id-388989

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  12. mRuby-C1

    Plasmid
    #54552
    Purpose
    Localization: C1 Cloning Vector, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mRuby
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. pBJ4

    Plasmid
    #167133
    Purpose
    A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter
    Depositor
    Akira Mine
    Article
    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d
    Insert
    luxCDABE
    Use
    Luciferase
    Expression
    Bacterial
    Promoter
    Kanamycin promoter
    Available Since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167133-plasmid-map-sequence-id-330439

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Showing: 3581 - 3600 of 15985 results