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We narrowed to 14,145 results for: TIM

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Showing: 9001 - 9050 of 14145 results

  1. pENTRY4_ Dja6_1

    Plasmid
    #126894
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126894-plasmid-map-sequence-id-249699

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  2. pENTRY4_ ERF922

    Plasmid
    #126893
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126893-plasmid-map-sequence-id-249692

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  3. pHH0103 TCERG1L(ORF-2) WW domain #1

    Plasmid
    #104294
    Purpose
    Bacterial expression of WW domain #1 from TCERG1L(ORF-2)
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    TCERG1L(ORF-2) WW domain #1 (TCERG1L Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104294-plasmid-map-sequence-id-202572

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  4. pHH0103 WAC WW domain

    Plasmid
    #104272
    Purpose
    Bacterial expression of WW domain from WAC
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WAC WW domain (WAC Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104272-plasmid-map-sequence-id-202570

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  5. pHH0103 TCERG1 WW domain #2

    Plasmid
    #104240
    Purpose
    Bacterial expression of WW domain #2 from TCERG1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    TCERG1 WW domain #2 (TCERG1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104240-plasmid-map-sequence-id-204799

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  6. pHH0103 TCERG1 WW domain #3

    Plasmid
    #104241
    Purpose
    Bacterial expression of WW domain #3 from TCERG1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    TCERG1 WW domain #3 (TCERG1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104241-plasmid-map-sequence-id-204382

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  7. pHH0103 HYPC WW domain #1

    Plasmid
    #104231
    Purpose
    Bacterial expression of WW domain #1 from HYPC
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    HYPC WW domain #1 (PRPF40B Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104231-plasmid-map-sequence-id-204368

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  8. pHH0103 WWP1 WW domain #4

    Plasmid
    #104223
    Purpose
    Bacterial expression of WW domain #4 from WWP1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WWP1 WW domain #4 (WWP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104223-plasmid-map-sequence-id-204364

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  9. pHH0103 TCERG1 WW domain #1

    Plasmid
    #104239
    Purpose
    Bacterial expression of WW domain #1 from TCERG1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    TCERG1 WW domain #1 (TCERG1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104239-plasmid-map-sequence-id-204293

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  10. pHH0103 WWP1 WW domain #3

    Plasmid
    #104222
    Purpose
    Bacterial expression of WW domain #3 from WWP1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WWP1 WW domain #3 (WWP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104222-plasmid-map-sequence-id-204289

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  11. pHH0103 WWP1 WW domain #1

    Plasmid
    #104220
    Purpose
    Bacterial expression of WW domain #1 from WWP1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WWP1 WW domain #1 (WWP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104220-plasmid-map-sequence-id-204206

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  12. pHH0103 WWP1 WW domain #2

    Plasmid
    #104221
    Purpose
    Bacterial expression of WW domain #2 from WWP1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WWP1 WW domain #2 (WWP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104221-plasmid-map-sequence-id-204204

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  13. A6TSR

    Plasmid
    #50359
    Purpose
    expresses T.gondii MIC2(67-660) domains in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2-A6TSR
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A, N357S, N463S, N470D
    Promoter
    CHICK beta-actin promoter
    Available Since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pET-29b(+)_His-CBD-SpEndoH

    Plasmid
    #218196
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH
    Depositor
    Florence Pojer
    Insert
    EndoH
    Tags
    tufA-His-CBD
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 45-313
    Promoter
    T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218196-plasmid-map-sequence-id-431799

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  15. pmr101A-cPr-mNeonGreen (ErmR)

    Plasmid
    #177208
    Purpose
    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Yeast codon optimized
    Promoter
    Synthetic constitutive promoter
    Available Since
    March 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    177208-plasmid-map-sequence-id-356946

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  16. pmr101A-cPr-mNeonGreen (KmR)

    Plasmid
    #177209
    Purpose
    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Yeast codon optimized
    Promoter
    Synthetic constitutive promoter
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177209-plasmid-map-sequence-id-348605

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  17. pAAV-CaMKIIa-hM3D(Gq)-mCherry-WPRE-pA(Short)

    Plasmid
    #183705
    Purpose
    AAV to express hM3D(Gq)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit
    Depositor
    Jonathan Ploski
    Insert
    hM3D(Gq)
    Use
    AAV
    Tags
    mCherry
    Promoter
    CaMKIIa
    Available Since
    May 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183705-plasmid-map-sequence-id-360768

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  18. pBS-L1PA1CH_blast rescue

    Plasmid
    #69611
    Purpose
    Expresses a blasticidin tagged codon optimized L1PA1 designed to recover inserts
    Depositor
    Astrid Roy-Engel
    Insert
    L1PA1
    Tags
    blast rescue cassette
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    69611-plasmid-map-sequence-id-123344

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  19. pET-29b(+)_His-CBD-3C-EmPNGaseF

    Plasmid
    #218193
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseF
    Depositor
    Florence Pojer
    Insert
    PNGaseF
    Tags
    tufA-His-CBD-3C
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 41-354
    Promoter
    T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218193-plasmid-map-sequence-id-431798

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  20. pCDH-Flag-hS1pr1_Neo

    Plasmid
    #203709
    Purpose
    Overexpression of FLAG-S1PR1.
    Depositor
    Timothy Hla
    Insert
    Flag-hS1PR1 (S1PR1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available Since
    Aug. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    203709-plasmid-map-sequence-id-403684

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  21. pPICZaa-STE2-cohis

    Plasmid
    #133433
    Purpose
    Yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Expression
    Yeast
    Mutation
    C59S/C252A, Codon optimized for P. pastoris, and …
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133433-plasmid-map-sequence-id-267727

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  23. RABGEF1A-c014

    Plasmid
    #175478
    Purpose
    Protein expression in insect cells. Entire CDS, M1-G491. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    RABGEF1 (RABGEF1 Human)
    Tags
    His6-Zbasic-TEV
    Expression
    Insect
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175478-plasmid-map-sequence-id-353251

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  25. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid
    #121173
    Purpose
    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.
    Depositor
    Timo Otonkoski
    Insert
    dCas9-VP192-T2A-EGFP
    Use
    CRISPR and Lentiviral; Inducible by doxycycline
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121173-plasmid-map-sequence-id-234995

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  26. psubCAG-WPRE-hVEGF-D-Strept

    Plasmid
    #119227
    Purpose
    Plasmid encodes Strep-tagged full-length (unprocessed) VEGF-D of human origin to be used for stimulation of lymphangiogenesis in mice in vivo.
    Depositor
    Kari Alitalo
    Insert
    VEGFD (VEGFD Human)
    Use
    AAV
    Tags
    Strept
    Promoter
    CAG
    Available Since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    119227-plasmid-map-sequence-id-231678

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  27. p426TEF-LptTPS-A

    Plasmid
    #89468
    Purpose
    Expression of prespatane synthase LptTPS-A in S. cerevisiae for sesquiterpene production
    Depositor
    Jing-Ke Weng
    Insert
    LptTPS-A
    Tags
    none
    Expression
    Yeast
    Promoter
    pBR322 origin of replication for propagation in E…
    Available Since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89468-plasmid-map-sequence-id-176087

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  28. pBHM2641

    Plasmid
    #229525
    Purpose
    Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    CRISPR
    Tags
    t2a-mNeonGreen-AID*
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    TEF1
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229525-plasmid-map-sequence-id-459550

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  29. pBHM2647

    Plasmid
    #229989
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229989-plasmid-map-sequence-id-461139

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  30. pBHM2648

    Plasmid
    #229990
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229990-plasmid-map-sequence-id-461146

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  31. pBHM2649

    Plasmid
    #229991
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229991-plasmid-map-sequence-id-461155

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  32. m.3047-Left TALE-G1397-N-DddA11-mCherry

    Plasmid
    #211336
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3047-Left TALE-G1397-N-DddA11-mCherry
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211336-plasmid-map-sequence-id-415645

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  33. pAAV-CAG-mNeonGreen

    Plasmid
    #124101
    Purpose
    Expression of the fluorescent protein mNeonGreen in mammalian cells
    Depositor
    Pantelis Tsoulfas
    Insert
    mNeonGreen
    Use
    AAV
    Expression
    Mammalian
    Mutation
    Codon usage was optimized for Homo Sapiens
    Promoter
    CAG
    Available Since
    April 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124101-plasmid-map-sequence-id-241203

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  34. m.5147-Left TALE-G1397-N-DddA11-mCherry

    Plasmid
    #211338
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.5147-Left TALE-G1397-N-DddA11-mCherry
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211338-plasmid-map-sequence-id-415660

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  35. pDD288

    Plasmid
    #137017
    Purpose
    mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    mNG-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized mNG
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137017-plasmid-map-sequence-id-274039

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  36. PIP2-CFP(UST) sensor

    Plasmid
    #170357
    Purpose
    PH-CFP(UST) fusion used to measure realtime changes in PIP2 levels.
    Depositor
    Kees Jalink
    Insert
    PH-CFP(UST) (PLCD1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    170357-plasmid-map-sequence-id-336129

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  37. PIP2-YFP(UST) sensor

    Plasmid
    #170358
    Purpose
    PH-YFP(UST) fusion used to measure realtime changes in PIP2 levels.
    Depositor
    Kees Jalink
    Insert
    PH-YFP(UST) (PLCD1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    170358-plasmid-map-sequence-id-335511

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  38. pHH0103 FNBP3 WW domain #1

    Plasmid
    #104233
    Purpose
    Bacterial expression of WW domain #1 from FNBP3
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    FNBP3 WW domain #1 (PRPF40A Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104233-plasmid-map-sequence-id-204803

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  39. pDD291

    Plasmid
    #137019
    Purpose
    mKate2-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    mKate2-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized mKate2
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137019-plasmid-map-sequence-id-274037

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  41. pAAV-CamKIIa-C1V1::FusionRed::Kv2.1

    Plasmid
    #102771
    Purpose
    "Activate neurons with 2p stimulation"
    Depositor
    Karel Svoboda
    Insert
    C1V1::FusionRed::Kv2.1
    Use
    AAV
    Tags
    FusionRed and Kv2.1 traficking domain
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    102771-plasmid-map-sequence-id-239474

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  42. pDD316

    Plasmid
    #137020
    Purpose
    mTurq2-GLO^SEC^2xHA vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    mTurq2-GLO^SEC^2xHA + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    2xHA and C. elegans GLO-optimized mTurq2
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137020-plasmid-map-sequence-id-274032

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  43. pHH0103 KIBRA WW domain #1

    Plasmid
    #104229
    Purpose
    Bacterial expression of WW domain #1 from KIBRA
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    KIBRA WW domain #1 (WWOX Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104229-plasmid-map-sequence-id-204298

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  44. pHH0103 FNBP3 WW domain #2

    Plasmid
    #104234
    Purpose
    Bacterial expression of WW domain #2 from FNBP3
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    FNBP3 WW domain #2 (PRPF40A Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104234-plasmid-map-sequence-id-204379

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  45. MIC2

    Plasmid
    #50050
    Purpose
    expresses toxoplasma gondii MIC2 extracellular domain in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2
    Tags
    HIS, SBP, and TEV
    Expression
    Mammalian
    Mutation
    S158A, N357S, N463S, N470D
    Promoter
    CMV
    Available Since
    April 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  46. DBJS-p2.25

    Plasmid
    #246894
    Purpose
    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid encoding guide for UBAP2L Nterm.
    Depositor
    David Bartel
    Insert
    UBAP2L Nterm Guide RNA 1 (UBAP2L Human)
    Expression
    Mammalian
    Available Since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    246894-plasmid-map-sequence-id-490581

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  49. AAV-SYN-eGFP-oPRE

    Plasmid
    #218681
    Purpose
    AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.
    Depositor
    Bart Nieuwenhuis
    Insert
    eGFP
    Use
    AAV
    Expression
    Mammalian
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218681-plasmid-map-sequence-id-433352

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  50. pET-29b(+)_His-CBD-EmEndoF3

    Plasmid
    #218194
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF3
    Depositor
    Florence Pojer
    Insert
    EndoF3
    Tags
    tufA-His-CBD
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 41-329
    Promoter
    T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218194-plasmid-map-sequence-id-431796

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