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We narrowed to 13,583 results for: TIM

Showing: 9001 - 9050 of 13583 results

  3. ReaLiSM A1-43-GPIba+2cys

    Plasmid
    #59577
    Purpose
    express VWF A1-GPIba ReaLiSM construct for single molecule study
    Depositor
    Timothy Springer
    Insert
    VWF A1-linker-GPIba (VWF Human)
    Use
    Tags
    6His
    Expression
    Mammalian
    Mutation
    Promoter
    chick beta-actin
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. RepX_pET28a(+)

    Plasmid
    #172199
    Purpose
    Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.
    Depositor
    Taekjip Ha
    Article
    Arslan et al Science. 2015 Apr 17;348(6232):344-7. do
    Insert
    RepX superhelicase
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    C18L, C43S, C167V, S400C, C612A
    Promoter
    Available since
    March 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    172199-plasmid-map-sequence-id-356977

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  15. pDB4695

    Plasmid
    #171127
    Purpose
    A plasmid containing the codon-optimized OsTIR1-F74A, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system.
    Depositor
    Li-Lin Du
    Article
    Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.
    Insert
    OsTIR1-F74A
    Use
    Other
    Tags
    Expression
    Mutation
    non applicable
    Promoter
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171127-plasmid-map-sequence-id-341099

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  16. pZA16mflon

    Plasmid
    #75439
    Purpose
    Plasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    mf-lon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75439-plasmid-map-sequence-id-147407

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  19. pAL-rfp_T

    Plasmid
    #74075
    Purpose
    pAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA rfp (T)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Available since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74075-plasmid-map-sequence-id-141966

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  21. pZ8-T_dCas9

    Plasmid
    #74062
    Purpose
    pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    dcas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    74062-plasmid-map-sequence-id-137972

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  23. pLV-HuGDNF

    Plasmid
    #182946
    Purpose
    To expres Human GDNF with optimized codon usage for better expression
    Depositor
    Pantelis Tsoulfas
    Insert
    Human GDNF (GDNF Synthetic, Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182946-plasmid-map-sequence-id-360108

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  24. pUbiGUSPlus

    Plasmid
    #64402
    Purpose
    Ubiquitin promoter driving synthetic optimised GUSPlus gene. Can be used as a reporter of promoter activity in plants by replacing Ubi promoter with sequence of interest.
    Depositor
    Claudia Vickers
    Article
    Vickers et al Plant Cell Rep. 2003 Sep;22(2):135-40. E
    Insert
    ubiquitin (Ubi-1) promoter
    Use
    Tags
    GUSPlus reporter
    Expression
    Plant
    Mutation
    Promoter
    Ubi-1
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64402-plasmid-map-sequence-id-108183

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  29. pV1025

    Plasmid
    #111424
    Purpose
    Expression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)
    Depositor
    Gerald Fink
    Article
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111424-plasmid-map-sequence-id-212243

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  30. pNR220

    Plasmid
    #79541
    Purpose
    Input plasmid: expresses three serine recombinases downstream of three inducible promoters.
    Depositor
    Timothy Lu
    Article
    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.
    Inserts
    TP901
    BxbI
    A118
    Use
    Tags
    His tag and LAA degradation tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79541-plasmid-map-sequence-id-148753

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  33. pTXB1-FIS

    Plasmid
    #51564
    Purpose
    Expression of E. coli FIS for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    FIS
    Use
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51564-plasmid-map-sequence-id-78815

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  34. pLenti spCas9 T2A iRFP670 P2A puro

    Plasmid
    #122182
    Purpose
    The plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
    Depositor
    Raphael Gaudin
    Inserts
    spCas9 (NEWENTRY )
    iRFP670
    Use
    Lentiviral
    Tags
    T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    122182-plasmid-map-sequence-id-237289

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  36. pAAV-CBh-mKate2-IRES-MCS (C-gap-WT)

    Plasmid
    #105922
    Purpose
    AAV-mediated gene expression from CBh promoter. Contains left AAV2 ITR with an 11bp deletion in C region and canonical right AAV2 ITR
    Depositor
    Marcella Patrick
    Inserts
    mKate2
    IRES-Multiple cloning site
    Use
    AAV
    Tags
    Expression
    Mutation
    codon optimized
    Promoter
    CBh and same CBh promoter as mKate2
    Available since
    Feb. 15, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    105922-plasmid-map-sequence-id-200599

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  37. pLenti spCas9 T2A mNeonGreen P2A puro

    Plasmid
    #122183
    Purpose
    The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
    Depositor
    Raphael Gaudin
    Inserts
    spCas9 (NEWENTRY )
    mNeonGreen
    Use
    Lentiviral
    Tags
    T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    122183-plasmid-map-sequence-id-241282

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Showing: 9001 - 9050 of 13583 results