We narrowed to 63,680 results for: cloning
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Plasmid#106430PurposeExpresses SpdCas9 from the nEF promoter in AAV backboneDepositorInsertnEF-dCas9
UseAAV and CRISPRTagsHA-NLS and NLSExpressionMammalianPromoternEFAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE3G-SOX17
Plasmid#104541PurposePiggyBac vector encoding doxycycline-inducible human SOX17DepositorAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SUV[SET]-dCas9
Plasmid#100088PurposeCatalytic domain [SET] of human SUVAR39H1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSUVAR39H1 (SUV39H1 Human)
UseCRISPRTags3XFLag-NLS-SUV[SET]-dCas9-NLSExpressionMammalianMutationdeleted aa 1-76PromoterCMVAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGPTVII-Bar-U-MatryoshCaMP6s
Plasmid#100024PurposePlant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
ExpressionPlantPromoterAtUBQ10Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-CDH5-mRuby3-IRES-puro
Plasmid#85144Purposelentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3DepositorInsertCDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)
UseLentiviralTagsmRuby3PromoterCAGAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-13:FBL-mEGFP
Plasmid#87427PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human FBLDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFBL Homology Arms with linker-mEGFP (FBL Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available sinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pLenti-V5-KDM3A
Plasmid#82331Purposelentiviral expression of KDM3ADepositorInsertKDM3A (KDM3A Human)
UseLentiviralTagsV5ExpressionMammalianMutationtwo silent mutations to make it siRNA resistantAvailable sinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMZ379
Plasmid#74215PurposeCombined sgRNA/Cas9 vector with NatMX markerDepositorInsertsCas9
rrk1:sgRNA
UseCRISPRExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTKW106alp7A
Plasmid#69360PurposeStabilized lacZ expression vector containing alp7A and hok/sokDepositorInsertslacZ
alp7A
hok
placIq-lacI
ExpressionBacterialPromoterpTac and placIqAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTK305
Plasmid#66812PurposeIVT vector for Fluc mRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFirefly luciferase
UseLuciferase; Mrna ivtPromoterT7Available sinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-ATAD2
Plasmid#65370PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertATAD2 (ATAD2 Human)
TagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMU-NUCr
Plasmid#61168PurposeFluorescent reporter for nucleus NLS-mCherry-GUS expressed under AtUBQ10 promoterDepositorInsertNLS-mCherry-GUS
ExpressionPlantPromoterAtUBQ10Available sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA
Plasmid#55202PurposePlasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdsRed2
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR.hU6hH1
Plasmid#32686Purposedual shRNA expression cassette entry vectorDepositorTypeEmpty backboneUseRNAiExpressionMammalianPromoterhU6hH1Available sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-MCU-V5-HIS
Plasmid#31731DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMCU (MCU Human)
TagsV5-HIS6XExpressionMammalianPromoterCMVAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti6/V5-DEST-HMGB1
Plasmid#31208DepositorAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVU/myc
Plasmid#24179DepositorInsertChloramphenicol resistance gene (CmR) - ccdB gene
UseGateway Cloning and LentiviralTagsMycExpressionMammalianAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-YES1
Plasmid#23938DepositorInsertYES1 (YES1 Human)
UseGateway CloningAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223-TRPM7
Plasmid#23476DepositorInsertTRPM7 (TRPM7 Human)
UseGateway CloningMutationInsert encodes NCBI: NR_149154.2 bases 265 to 146…Available sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MAPK1
Plasmid#23498DepositorAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by