We narrowed to 15,052 results for: pET

1. pEN019-SpCas9-hiNLS-t-M1M4

   Plasmid

   #234456

   Purpose

   Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

   Depositor

   Ross Wilson

   Article

   Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

   Insert

   SpCas9 hiNLS t-M1M4
   

   Use

   CRISPR

   Tags

   His6, Nucleoplasmin, SV40, and c-Myc

   Expression

   Bacterial

   Mutation

   Cys at positions 80 and 574 have been mutated to …

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. OA-1136J

   Plasmid

   #153023

   Purpose

   Express His-MBP-TEV-CasRx recombinant protein

   Depositor

   Omar Akbari

   Article

   Brogan et al ACS Sens. 2021 Nov 26;6(11):3957-3966. d

   Insert

   CasRx
   

   Tags

   His-MBP-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AURKA_HUMAN_D0

   Plasmid

   #79739

   Purpose

   This plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   AURKA (AURKA Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pEE050

   Plasmid

   #176807

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   601
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEE060

   Plasmid

   #176817

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   611
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NEK1_HUMAN_D0

   Plasmid

   #79725

   Purpose

   This plasmid encodes the kinase domain of NEK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   NEK1 (NEK1 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

7. MERTK_HUMAN_D0

   Plasmid

   #79705

   Purpose

   This plasmid encodes the kinase domain of MERTK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   MERTK (MERTK Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pSNAP-CAPZ

   Plasmid

   #69948

   Purpose

   Simultaneous expression of a His- and SNAP-tagged β1 subunit and untagged α1 subunits of chicken CapZ

   Depositor

   Jeff Gelles

   Article

   Bombardier et al Nat Commun. 2015 Nov 13;6:8707. doi: 10.

   Inserts

   CapZ beta 1 (CAPZB Chicken)
   CapZ alpha 1 (CAPZA1 Chicken)
   

   Tags

   His 6 and SNAP

   Expression

   Bacterial

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MARK3_HUMAN_D0

   Plasmid

   #79740

   Purpose

   This plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   MARK3 (MARK3 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pEE065

    Plasmid

    #176822

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    704
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LIC1B_Caspase-LOVN7C-7 C450A

    Plasmid

    #104628

    Purpose

    bacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutation

    Depositor

    Jim Wells

    Article

    Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    Caspase-3 (CASP3 Human)
    

    Tags

    His and LOV2

    Expression

    Bacterial

    Mutation

    C450A (dark-locked and light-insensitive mutation)

    Promoter

    T7

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. KCC2G_HUMAN_D0

    Plasmid

    #79711

    Purpose

    This plasmid encodes the kinase domain of KCC2G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KCC2G (CAMK2G Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. EPHB1_HUMAN_D0

    Plasmid

    #79694

    Purpose

    This plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    EPHB1 (EPHB1 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. STK3_HUMAN_D0

    Plasmid

    #79689

    Purpose

    This plasmid encodes the kinase domain of STK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    STK3 (STK3 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pGST-HsSyx-375-792

    Plasmid

    #179292

    Purpose

    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Tags

    His6 + GST + TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    residues 375-792 from accession number NP_0010361…

    Promoter

    T7

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEE062

    Plasmid

    #176819

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    701
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEE077

    Plasmid

    #176834

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    716
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GAK_HUMAN_D0

    Plasmid

    #79682

    Purpose

    This plasmid encodes the kinase domain of GAK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    GAK (GAK Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. KC1D_HUMAN_D0

    Plasmid

    #79723

    Purpose

    This plasmid encodes the kinase domain of KC1D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KC1D (CSNK1D Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. CDKL1_HUMAN_D0

    Plasmid

    #79699

    Purpose

    This plasmid encodes the kinase domain of CDKL1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CDKL1 (CDKL1 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

21. FGFR3_HUMAN_D0

    Plasmid

    #79731

    Purpose

    This plasmid encodes the kinase domain of FGFR3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    FGFR3 (FGFR3 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pSUMO-HsSyx3

    Plasmid

    #179288

    Purpose

    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + SUMO + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Tags

    His6 + SUMO + TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    residues 393-792 from accession number NP_0010361…

    Promoter

    T7

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CDK16_HUMAN_D0

    Plasmid

    #79696

    Purpose

    This plasmid encodes the kinase domain of CDK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CDK16 (CDK16 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

24. actin CP alpha 2 beta 2

    Plasmid

    #13452

    Depositor

    John Cooper

    Article

    Bhattacharya et al J Biol Chem. 2006 Oct 13. 281(41):31021-

    Insert

    capping protein alpha 2 beta 2 (mouse) (Capzb, Capza2 Mouse)
    

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

25. pEE038

    Plasmid

    #176795

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    407
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEE048

    Plasmid

    #176805

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    504
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CHK2_HUMAN_D0

    Plasmid

    #79715

    Purpose

    This plasmid encodes the kinase domain of CHK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CHK2 (CHEK2 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

28. DAPK1_HUMAN_D0

    Plasmid

    #79728

    Purpose

    This plasmid encodes the kinase domain of DAPK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    DAPK1 (DAPK1 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

29. BMPR2_HUMAN_D0

    Plasmid

    #79709

    Purpose

    This plasmid encodes the kinase domain of BMPR2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    BMPR2 (BMPR2 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

30. KC1E_HUMAN_D0

    Plasmid

    #79724

    Purpose

    This plasmid encodes the kinase domain of KC1E. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KC1E (CSNK1E Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only