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We narrowed to 14,145 results for: TIM

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Showing: 9051 - 9100 of 14145 results

  1. pET-29b(+)_His-CBD-EmEndoF1

    Plasmid
    #218195
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF1
    Depositor
    Florence Pojer
    Insert
    EndoF1
    Tags
    tufA-His-CBD
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 51-339
    Promoter
    T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218195-plasmid-map-sequence-id-431792

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  2. m.3075-Left TALE-G1397-N-DddA11-mCherry

    Plasmid
    #211337
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3075-Left TALE-G1397-N-DddA11-mCherry
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211337-plasmid-map-sequence-id-415646

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  3. pT7CFE1_EVA71_VP12A

    Plasmid
    #212977
    Purpose
    Encodes EV-A71 4643 VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Enterovirus A71 4643 VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212977-plasmid-map-sequence-id-420362

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  4. pT7CFE1_EVD68_VP12A

    Plasmid
    #212984
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212984-plasmid-map-sequence-id-420357

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  5. pT7CFE1_EVD68_3ABC

    Plasmid
    #212979
    Purpose
    ncodes EV-D68 MO/14/18947 3ABC optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABC
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212979-plasmid-map-sequence-id-420356

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  6. pT7CFE1_EVD68_3ABCD

    Plasmid
    #212981
    Purpose
    Encodes EV-D68 MO/14/18947 3ABCD optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABCD
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212981-plasmid-map-sequence-id-420354

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  7. pCCL-c-MNDU3-CD3D-274+5G>A

    Plasmid
    #198023
    Purpose
    LV Expression of CD3D cDNA with mutation at c.274+5G>A used as a disease editing target for CD3d SCID mutation identified in Ecuador
    Depositor
    Donald Kohn
    Insert
    CD3D c.274+5G>A
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Codon optimized (20 bp) on either end of cDNA for…
    Available Since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    198023-plasmid-map-sequence-id-390930

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  8. pCCL-c-MNDU3-CD3D-275-2A>G

    Plasmid
    #198024
    Purpose
    LV Expression of CD3D cDNA with mutation at c.275-2A>G used as a disease editing target for CD3d SCID mutation identified in Japan
    Depositor
    Donald Kohn
    Insert
    CD3D c.275-2A>G
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Codon optimized (20 bp) on either end of cDNA for…
    Available Since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198024-plasmid-map-sequence-id-390335

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  9. pAAV-CaMKIIa-hM4D(Gi)-mCherry-WPRE-pA(Short)

    Plasmid
    #183704
    Purpose
    AAV to express hM4D(Gi)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit
    Depositor
    Jonathan Ploski
    Insert
    hM4D(Gi)
    Use
    AAV
    Tags
    mCherry
    Promoter
    CaMKIIa
    Available Since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    183704-plasmid-map-sequence-id-360830

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  10. SYKA-c014

    Plasmid
    #175488
    Purpose
    Protein expression in insect cells. Entire CDS, M1-N635. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    SYK (SYK Human)
    Tags
    His6-Zbasic-TEV
    Expression
    Insect
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175488-plasmid-map-sequence-id-353252

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  11. pANY4-pL18-ccdB

    Plasmid
    #164898
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Tags
    His-tag
    Expression
    Bacterial
    Available Since
    April 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164898-plasmid-map-sequence-id-327989

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  12. pDD289

    Plasmid
    #137018
    Purpose
    GFP-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    GFP-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized GFP
    Expression
    Worm
    Available Since
    April 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    137018-plasmid-map-sequence-id-291422

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  13. pHH0103 PQBP1 WW domain

    Plasmid
    #104287
    Purpose
    Bacterial expression of WW domain from PQBP1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    PQBP1 WW domain (PQBP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104287-plasmid-map-sequence-id-203724

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  14. pHH0103 HLA-DQA2 WW domain

    Plasmid
    #104297
    Purpose
    Bacterial expression of WW domain from HLA-DQA2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    HLA-DQA2 WW domain (HLA-DQA2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104297-plasmid-map-sequence-id-203688

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  16. pHH0103 HYPC WW domain #2

    Plasmid
    #104232
    Purpose
    Bacterial expression of WW domain #2 from HYPC
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    HYPC WW domain #2 (PRPF40B Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104232-plasmid-map-sequence-id-204196

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  17. m2ap

    Plasmid
    #50051
    Purpose
    expresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cells
    Depositor
    Timothy Springer
    Insert
    M2AP
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    starts at aa47; N127R, S158A, S177N, N357S, N463S…
    Promoter
    CMV
    Available Since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. AAV-sCAG-mBaoJin-oPRE

    Plasmid
    #218684
    Purpose
    AAV vector plasmid expressing mBaoJin under a short variant of the CAG (sCAG) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.
    Depositor
    Bart Nieuwenhuis
    Insert
    mBaoJin
    Use
    AAV
    Expression
    Mammalian
    Promoter
    Short variant of the CAG promoter
    Available Since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    218684-plasmid-map-sequence-id-438234

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  20. PKMYT1

    Plasmid
    #39061
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PKMYT1 (PKMYT1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  21. AAV-SYN-mBaoJin-oPRE

    Plasmid
    #218683
    Purpose
    AAV vector plasmid expressing mBaoJin under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.
    Depositor
    Bart Nieuwenhuis
    Insert
    mBaoJin
    Use
    AAV
    Expression
    Mammalian
    Promoter
    Human synapsin promoter
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    218683-plasmid-map-sequence-id-447610

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  23. pLV-DBNF-mCherry

    Plasmid
    #187195
    Purpose
    To express the mouse BDNF tagged with the FP mCherry
    Depositor
    Pantelis Tsoulfas
    Insert
    BDNF
    Use
    Lentiviral
    Tags
    fluorescent protein mCherry
    Expression
    Mammalian
    Mutation
    The codons have been optimized for better express…
    Promoter
    CMV
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187195-plasmid-map-sequence-id-374465

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  24. pcDNA3-HA-p48

    Plasmid
    #11614
    Depositor
    Steven Johnson
    Insert
    Interferon-Stimulated Gene Factor 3 (IRF9 Human)
    Tags
    HA
    Expression
    Mammalian
    Available Since
    July 18, 2007
    Availability
    Academic Institutions and Nonprofits only

  25. pmCellFree_KA1_SARS-COV-2_NSP16

    Plasmid
    #169389
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP16 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP16
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169389-plasmid-map-sequence-id-336165

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  26. pSMH1741

    Plasmid
    #160930
    Purpose
    Expression of soluble Sc octamer (H3)
    Depositor
    Stephen Harrison
    Inserts
    HHF1
    HTA1
    HTB1
    HHT1
    Tags
    6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Available Since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160930-plasmid-map-sequence-id-318962

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  27. pSMH1742

    Plasmid
    #160929
    Purpose
    Expression of soluble Sc octamer (Cse4)
    Depositor
    Stephen Harrison
    Inserts
    HHF1
    HTA1
    HTB1
    Cse4
    Tags
    6His-TEV, N-terminal 6His-TEV, and none
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Available Since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160929-plasmid-map-sequence-id-319094

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  28. FUG-P2A-tC3-W

    Plasmid
    #129475
    Purpose
    3rd-generation lentiviral vector for hUbC-driven co-expression of EGFP and truncated C3
    Depositor
    Robert Gross
    Insert
    truncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    human ubiquitin C
    Available Since
    Feb. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    129475-plasmid-map-sequence-id-384588

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  30. lentiCRISPRv2_PROX1_1+3

    Plasmid
    #121176
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121176-plasmid-map-sequence-id-235001

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  31. pT7CFE1_polio_3ABC

    Plasmid
    #212986
    Purpose
    Encodes Poliovirus type I Mahoney 3ABC optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney 3ABC
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212986-plasmid-map-sequence-id-420355

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  32. GBH-R-cmlc2GFP.1

    Plasmid
    #188004
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker cmlc2 GFP
    Use
    Targetable insertional mutagen and reporter system
    Promoter
    Endogenuous Promoter; cmlc2 promotor
    Available Since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188004-plasmid-map-sequence-id-381042

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  33. rnALDH

    Plasmid
    #67920
    Purpose
    Expresses aldehyde dehydrogenase from Rattus norvegicus, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Insert
    Rattus norvegicus aldehyde dehydrogenase (Aldh3a1 Rat)
    Tags
    6x-His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  34. GBH-R-gcryGFP.3

    Plasmid
    #188000
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available Since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188000-plasmid-map-sequence-id-381121

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  35. SPR

    Plasmid
    #39161
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase) (SPR Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  37. pCAGGS-Flag-S1pr1-R120A

    Plasmid
    #203707
    Purpose
    Overexpression of FLAG-S1PR1-R120A mutant.
    Depositor
    Timothy Hla
    Insert
    Flag-S1PR1-R120A (S1PR1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    R120A
    Available Since
    Dec. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    203707-plasmid-map-sequence-id-417438

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  38. PBRM1

    Plasmid
    #39013
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PBRM1 (PBRM1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. PBRM1

    Plasmid
    #39027
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    polybromo 1 [isoform a] (PBRM1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  40. pmCellFree_KA1_SARS-COV-2_NSP10

    Plasmid
    #169384
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP10 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP10
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169384-plasmid-map-sequence-id-336496

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  42. PBRM1

    Plasmid
    #38999
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PBRM1 (PBRM1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  43. psubCMV-mVEGF-D-dNdC-WPRE

    Plasmid
    #119228
    Purpose
    Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has native signal peptide to be used for stimulation of lymphangiogenesis in mice in vivo.
    Depositor
    Kari Alitalo
    Insert
    Vegfd-dN-dC (Vegfd Mouse)
    Use
    AAV
    Promoter
    CMV
    Available Since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    119228-plasmid-map-sequence-id-231686

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  44. pmCellFree_KA1_SARS-COV-2_ORF3b

    Plasmid
    #169391
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3b with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF3b
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169391-plasmid-map-sequence-id-336874

    Enlarge

  46. PBRM1

    Plasmid
    #39028
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PBRM1 (PBRM1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  50. PBRM1

    Plasmid
    #39030
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    polybromo 1 [isoform a] (PBRM1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 9051 - 9100 of 14145 results