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pQMD

Plasmid

#48103

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of myc-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

myc tag

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm_P1T_DP-A21

Plasmid

#90318

Purpose

Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS A21

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Type

Empty backbone

Expression

Bacterial

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRU1105

Plasmid

#14469

Depositor

Philip Poole

Article

Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

Insert

DsRedT3

Expression

Bacterial

Available Since

April 23, 2007

Availability

Academic Institutions and Nonprofits only

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pMApra-Ptol

Plasmid

#218359

Purpose

Empty vector with XylS-Pm expression system

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-AviTag

Plasmid

#67175

Purpose

CRISPaint universal donor for AviTag fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

AviTag

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP-ura4MX6

Plasmid

#33140

Purpose

genomic GFP tagging in yeast

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Inserts

ura4MX6
GFP

Available Since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pXWStetO

Plasmid

#160824

Purpose

1 repeat of tetO sponge

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

tetO-t

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pDZ251 (pKAN 24xPP7SL cyc)

Plasmid

#35192

Depositor

Robert Singer

Article

Larson et al Science. 2011 Apr 22;332(6028):475-8.

Insert

24xPP7SL cyc

Available Since

Feb. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pCD315

Plasmid

#113321

Purpose

pBAD-W108 scRNA.b1

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

scRNA.b1_MS2

Use

CRISPR

Expression

Bacterial

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pEZ01

Plasmid

#81124

Purpose

pACSA_Pcpt_gfp

Depositor

Brian Pfleger

Article

Zess et al Biotechnol Bioeng. 2016 Feb;113(2):424-3

Insert

GFP

Promoter

Pcpt

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pFLlR5

Plasmid

#149481

Purpose

Broad-Host range expression vector, RSF1010 origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LacI-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLlacO-1

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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JDS70

Plasmid

#32285

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Type

Empty backbone

Use

T7

Tags

3X Flag and FOKI WT

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pFVR5

Plasmid

#149489

Purpose

Broad-Host range expression vector, RSF1010 origin, VanR(AM) repressor, PVanCC promoter, mRFP, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEZ10

Plasmid

#81135

Purpose

pACSA_J23113_tetR PEZ3_gfp

Depositor

Brian Pfleger

Article

Zess et al Biotechnol Bioeng. 2016 Feb;113(2):424-3

Insert

GFP

Promoter

PEZ3

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pLO86

Plasmid

#91724

Purpose

mycobacterial expression of mCherry reporter

Depositor

Graham Hatfull

Article

Oldfield et al J Bacteriol. 2014 Oct;196(20):3589-97. d

Type

Empty backbone

Use

Unspecified; Mycobacterial expression

Tags

mCherry

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFCyR5

Plasmid

#149480

Purpose

Broad-Host range expression vector, RSF1010 origin, CymR(AM) repressor, PCymRC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-12Pk-LEU2MX6

Plasmid

#49179

Purpose

genomic 12Pk epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

12Pk

Expression

Yeast

Available Since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pDR3.10

Plasmid

#19367

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

msp-142 ORF+3' in pDONR201

Expression

Worm

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

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pH6HTC_NASP-Halo

Plasmid

#175340

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

NASP (NASP Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 Grc3

Plasmid

#63760

Purpose

yeast ORF Grc3 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Grc3

Use

Gateway donor vector

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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