We narrowed to 8,304 results for: construct

1. iLID-mRuby3-iTrkA

   Plasmid

   #136510

   Purpose

   Mammalian expression of iLID fused to mRuby3 and intracellular signaling domain of TrkA (aa 450-799)

   Depositor

   Bianxiao Cui

   Article

   Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

   Insert

   iLID mRuby3 iTrkA (Ntrk1 Rat, Avena sativa)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. iLID-mRuby3-iTrkB

   Plasmid

   #136511

   Purpose

   Mammalian expression of iLID fused to mRuby3 and intracellular signaling domain of TrkB (aa 455-822)

   Depositor

   Bianxiao Cui

   Article

   Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

   Insert

   iLID mRuby3 iTrkB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFlare9A-CD45i4e5i5

   Plasmid

   #131834

   Purpose

   A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a CTCF binding site.

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   PTPRC (PTPRC Human)
   

   Use

   Reporter construct

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PROM5_MprbcL_short_chlo

   Plasmid

   #165161

   Purpose

   L0 part - promoter

   Depositor

   Jim Haseloff

   Article

   Frangedakis et al ACS Synth Biol. 2021 Jul 16;10(7):1651-1

   Insert

   Marchantia polymorpha rbcL promoter region
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. 5UTR_Mprbcl_chlo

   Plasmid

   #163934

   Purpose

   L0 part - 5'UTR

   Depositor

   Jim Haseloff

   Article

   Frangedakis et al ACS Synth Biol. 2021 Jul 16;10(7):1651-1

   Insert

   Marchantia polymorpha rbcL 5'UTR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. PROM_Ntpsba_chlo

   Plasmid

   #163950

   Purpose

   L0 part - promoter

   Depositor

   Jim Haseloff

   Article

   Frangedakis et al ACS Synth Biol. 2021 Jul 16;10(7):1651-1

   Insert

   Nicotiana tabacum psbA promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. 5UTR_Mppetb_short_chlo

   Plasmid

   #163933

   Purpose

   L0 part - 5'UTR

   Depositor

   Jim Haseloff

   Article

   Frangedakis et al ACS Synth Biol. 2021 Jul 16;10(7):1651-1

   Insert

   Marchantia polymorpha petB 5'UTR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pCsB_spacer

   Plasmid

   #136076

   Purpose

   Use when making odd level constructs (L3,...) if pCsB position (position 2) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Even spacer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pCsA_spacer

   Plasmid

   #136075

   Purpose

   Use when making odd level constructs (L3,...) if pCsA position (position 1) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Even spacer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pGBW-m4136457

    Plasmid

    #149537

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pGBW-m4046858

    Plasmid

    #145658

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pGBW-m4046730

    Plasmid

    #145659

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pGBW-m4046823

    Plasmid

    #145634

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pGBW-m4046816

    Plasmid

    #145622

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pGBW-m4046791

    Plasmid

    #145586

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGBW-m4046774

    Plasmid

    #145574

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGBW-m4046846

    Plasmid

    #145575

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pBV163

    Plasmid

    #108364

    Purpose

    Used to replace C. glabrata YAP1 promoter with the stronger TDH3 promoter.

    Depositor

    Scott Moye-Rowley

    Article

    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

    Insert

    Knock-in Cg TDH3 promoter in Cg YAP1 gene
    

    Use

    Replace c.glabrata yap1 promoter with c. glabrata…

    Tags

    Expression

    Mutation

    Promoter

    TDH3

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TF101-STTM-miR394

    Plasmid

    #84813

    Purpose

    Turning off/down miR394 in Soybean

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM-miR394
    

    Use

    Tags

    Expression

    Plant

    Mutation

    bp8 is deleted- please see depositor comments bel…

    Promoter

    unknown

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TF101-STTM-miR390

    Plasmid

    #84812

    Purpose

    Turning off/down miR390 in Soybean

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM-miR390
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    unknown

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge