We narrowed to 8,446 results for: gnal

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pcDNA3.1-Flag-gp130dimer-stm2585

Plasmid

#174395

Purpose

Constitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

IL6ST cytosolic domain and sarA chimera

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-stm2585

Plasmid

#174391

Purpose

S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with N-terminal Flag tag

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

stm2585

Tags

Flag

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLIC-His-GBAi

Plasmid

#171755

Purpose

For the expression of His-GBAi in bacteria

Depositor

Mikel Garcia-Marcos

Article

Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

Insert

GBAi

Tags

His

Expression

Bacterial

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-N1-mSNX27-H112A RNAi-resistant

Plasmid

#163621

Purpose

mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 and with a point mutation H112A C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27-H112A RNAi-resistant (Snx27 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

H112A and RNAi resistance

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Ad-HisFlag-USP19ER

Plasmid

#155244

Purpose

for generating adenoviruses expressing the ER isoform of rat USP19

Depositor

Simon Wing

Article

Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

Insert

USP19 (ER isoform) (Usp19 Rat)

Use

Adenoviral

Tags

6XHis-3XFlag

Mutation

H118R- please see dep. comments

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSWD95Y14D

Plasmid

#159098

Purpose

CckA FRET Sensor with cyclic-di-GMP point mutant

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3

Expression

Bacterial

Mutation

Mutated Y514 to D514

Promoter

Xylose

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSWD95D293A

Plasmid

#159099

Purpose

CckA FRET Sensor with DITE motif mutant

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3

Expression

Bacterial

Mutation

CckA D293 mutated to A293 and V199I on mRuby3 (P…

Promoter

Xylose

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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HA-Casp-2L

Plasmid

#157932

Purpose

Over expression of Casp-2L

Depositor

Baoji Xu

Article

Xu et al Nat Commun. 2019 Aug 9;10(1):3622. doi:

Insert

Casp2 (Casp2 Mouse)

Tags

HA and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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TB:3xDBE_RE:MiniP

Plasmid

#124529

Purpose

3 Copies of the Daf-16 family binding element

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

3 copies of the Daf-16 family binding element

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA

Plasmid

#140223

Purpose

Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activator

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein

Expression

Mammalian

Mutation

D832A

Promoter

PGK

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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HsH1R DRY->AAY-mTurquoise2

Plasmid

#137786

Purpose

Visualization of the Histamine-1 receptor DRY mutant

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Human histamine receptor; DRY mutant

Expression

Mammalian

Mutation

DRY -> AAY

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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HsH1R DRY->AAY-mCherry

Plasmid

#137788

Purpose

Visualization of the Histamine-1 receptor DRY mutant

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Human histamine receptor; DRY mutant

Expression

Mammalian

Mutation

DRY -> AAY

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-RnBarr2

Plasmid

#137798

Purpose

Visualization of beta-arrestin2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin2 (Arrb2 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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HsH1R DRY->AAY-p2A-mCherry

Plasmid

#137787

Purpose

Visualization of the Histamine-1 receptor DRY mutant

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Human histamine receptor; DRY mutant

Expression

Mammalian

Mutation

DRY -> AAY

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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RnAT1aR DRY->AAY-mCherry

Plasmid

#137783

Purpose

Visualization of the At1aR DRY mutant

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat At1a receptor; DRY mutant (Agtr1a Rat)

Expression

Mammalian

Mutation

DRY -> AAY

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFP-RIP2_S176E

Plasmid

#131203

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

Insert

RIPK2 S176E (RIPK2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S176E

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFP-RIP2_Y474F

Plasmid

#131204

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

Insert

RIPK2 Y474F (RIPK2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Y474F

Promoter

pCMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 mSmoothened NQ4

Plasmid

#126407

Purpose

Expression of Smoothened NQ4 in mammalian cell lines.

Depositor

Stacey Ogden

Article

Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

Insert

m-Smoothened (Smo Mouse)

Expression

Mammalian

Mutation

Smo glycosylation site NQ4

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 mSmoothened NQ3

Plasmid

#126406

Purpose

Expression of Smoothened NQ3 in mammalian cell lines.

Depositor

Stacey Ogden

Article

Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

Insert

m-Smoothened (Smo Mouse)

Expression

Mammalian

Mutation

Smo glycosylation site NQ3

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK502

Plasmid

#121457

Purpose

CEN HIS3 CNB1

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Promoter

Endogenous genomic promoter region

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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