159,481 results

1. pCAGGS-PIF3-mEGFP

   Plasmid

   #100283

   Purpose

   Expresses PIF3 (1-100 aa) fused with mEGFP in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome interacting factor 3 (PIF3 Mustard Weed)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   N-terminus (1-100 aa) of PIF3 is used

   Promoter

   CAG promoter

   Available since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSMPUW_6.16_ M4_Ef1a-gateway-V5-SV40-Zeo

   Plasmid

   #128452

   Purpose

   lentiviral destination vector with Ef1a promoter, C-terminal V5 tag, SV40 promoter, and Zeo Resistance gene

   Depositor

   Lindy Barrett

   Insert

   Ef1a promoter, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pH6HTC_HSPA9-Halo

   Plasmid

   #175332

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   HSPA9 (HSPA9 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMIA10.53-Clover

   Plasmid

   #214450

   Purpose

   BxbI landing-pad mClover-IRES-puro donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   mClover
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-hRIPK1-Flag-delC

   Plasmid

   #112493

   Purpose

   Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

   Depositor

   Junying Yuan

   Insert

   RIPK1 (RIPK1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deletion of C terminus (AA290-671 deleted)

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHiFi Cas9-2×sgRNA (empty, donor)

   Plasmid

   #162277

   Purpose

   All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.

   Depositor

   Kazuhisa Nakayama

   Article

   Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi

   Insert

   HiFi Cas9
   

   Use

   CRISPR

   Tags

   BPNLS and FLAG tag

   Expression

   Mammalian

   Mutation

   SpCas9 (R691A)

   Promoter

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-P-RAN-GFP1

   Plasmid

   #106408

   Purpose

   P-RAN-GFP1 (RANbody GFP1::vHRP)

   Depositor

   Joshua Sanes

   Article

   Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

   Insert

   P-RAN-GFP1
   

   Use

   Tags

   HA and His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCVpuro-hAIM2-DsRed monomer

   Plasmid

   #51609

   Purpose

   Express DsRed monomer-tagged hAIM2 in mammalian cells

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   AIM2 (AIM2 Human)
   

   Use

   Retroviral

   Tags

   DsRed monomer

   Expression

   Mammalian

   Mutation

   Promoter

   MESV

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTn7-SCOUT11.G

   Plasmid

   #200999

   Purpose

   pLv1-pUC18R6KT-miniTn7-Gm-sfGFP, GmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Insert

   superfolder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23104

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. dCas9-CRY2Clust-FUSN

    Plasmid

    #200967

    Purpose

    induction of phase-separated condensate mediated by FUSN-IDR

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    FUSN
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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