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pZ_P-GAP-ALD1

Plasmid

#126711

Purpose

yeast plasmid complementing ALD1 from Komagataella phaffii

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

ald1

Use

Tags

6xHis,Myc

Expression

Yeast

Mutation

Promoter

GAP

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-GAP-ADH3

Plasmid

#126710

Purpose

yeast plasmid complementing ADH3 from Komagataella phaffii

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

adh3

Use

Tags

6xHis,Myc

Expression

Yeast

Mutation

Promoter

GAP

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-deltaA1-R1597W-VWF

Plasmid

#124826

Purpose

Expresses multimeric human VWF protein without A1 domain and a R1597W mutation in A2 domain. This protein has C-terminal c-Myc tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain and R1597W mutation A2 domain (VWF Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-deltaA1-D1498A-VWF

Plasmid

#124825

Purpose

Expresses multimeric human VWF protein without A1 domain and a D1498A mutation in A2 domain. This protein has C-terminal c-Myc tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain and D1498A mutation A2 domain (VWF Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pGM190

Plasmid

#69614

Purpose

inducible gene expression in Streptomyces

Depositor

Günther Muth

Article

Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

Inserts

aphII
tsr
rep
sso
TipA promoter
dso

Use

Streptomyces- e. coli shuttle vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pEA01

Plasmid

#170766

Purpose

Utilisation of pSAM2 excision tool in Amycolatopsis spp

Depositor

Jean-Luc Pernodet

Article

Santos et al Microorganisms. 2022 Apr 16;10(4). pii:

Inserts

xis, int
oriT
pac

Use

Tags

Expression

Bacterial

Mutation

Promoter

trcp

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pGM1202

Plasmid

#69615

Purpose

inducible gene expression in Streptomyces, C-terminal His-tag encoding sequence

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aac
tsr
rep
sso
TipA promoter
dso

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pGM1190

Plasmid

#69994

Purpose

OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aac
tsr
rep
sso
TipA promoter
dso

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGM202T7

Plasmid

#69993

Purpose

E. coli/Streptomyces expression vector containing the T7-promoter

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aphII
tsr
rep
sso
T7 promoter
dso
His-Orf

Use

Tags

N-terminal and C-terminal His tag encoding sequen…

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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HA-GABARAP

Plasmid

#137760

Purpose

Expression of GABARAP

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GABARAP (GABARAP Human)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4-FLAG-Jhdm2a

Plasmid

#38136

Depositor

Toru Nakano, Toshinobu Nakamura

Article

Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

Insert

Jhdm2a (Kdm3a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

protein starts at aa115

Promoter

CMV

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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HA-MAP1LC3C

Plasmid

#137758

Purpose

Expression of LC3C

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

MAP1LC3C (MAP1LC3C Human)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OK+9MS-Cas9

Plasmid

#120354

Purpose

piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

Depositor

Knut Woltjen

Article

Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

Insert

OK+9MS cassette

Use

CRISPR; Piggybac transposon

Tags

Expression

Mammalian

Mutation

Klf4 [1-483]

Promoter

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pmGFP-ADAR1-p150

Plasmid

#117927

Purpose

ADAR1-p150 expression plasmid.

Depositor

Kumiko Ui-Tei

Article

Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

Insert

ADAR1-p150 (ADAR Human)

Use

Tags

myc-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-XBP1 mNeonGreen NLS

Plasmid

#115968

Purpose

ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen

Depositor

David Andrews

Article

Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

Insert

X-box binding protein 1 (XBP1 Synthetic, Human)

Use

Lentiviral

Tags

HA tag, c-myc NLS, and mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pmGFP-ADAR1-p110

Plasmid

#117928

Purpose

ADAR1-p110 expression plasmid.

Depositor

Kumiko Ui-Tei

Article

Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

Insert

ADAR1-p110 (ADAR Human)

Use

Tags

myc-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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hFLT1

Plasmid

#83435

Purpose

human VEGFR1 with HA and FLAG tag

Depositor

Christie Thomas

Article

Raikwar et al Exp Cell Res. 2013 Oct 15;319(17):2578-8

Insert

human VEGFR1 (FLT1 Human)

Use

Tags

Flag and Myc

Expression

Mammalian

Mutation

HA-tag was inserted 30 AA downstream of the FLT1 …

Promoter

CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAG654 pFAST

Plasmid

#172861

Purpose

Expresses pFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

pFAST

Use

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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5HRE/GFP

Plasmid

#46926

Purpose

hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system

Depositor

Martin Brown, Thomas Foster

Article

Vordermark et al Neoplasia. 2001 Nov-Dec;3(6):527-34.

Insert

5X HRE of VEGF (VEGFA Human)

Use

Tags

d2EGFP

Expression

Mammalian

Mutation

five copies of a 35-bp fragment from the hypoxia-…

Promoter

EF-1α promoter

Available since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

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FR_HNF4A2

Plasmid

#31100

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF4A (HNF4A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

splice variant 2

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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