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BB3_arscen_URA3_Hygro

Plasmid

#118077

Purpose

low copy plasmid, neg. control

Depositor

Michael Sauer

Article

Steiger et al Metab Eng. 2019 Mar;52:224-231. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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SK-YFP-NM-A

Plasmid

#48664

Purpose

Bacterial NM repression YFP reporter: protospacer A

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

NM prototspacer A/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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SK-YFP-TD-A

Plasmid

#48666

Purpose

Bacterial TD repression YFP reporter: protospacer A

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TD prototspacer A/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pDL128

Plasmid

#43912

Depositor

Stephen Elledge

Article

Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

Insert

DIF1 (DIF1 Budding Yeast)

Expression

Yeast

Mutation

SML domain mutated PPMLINQRT->AAAAAAAA, S59G, …

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pDL127

Plasmid

#43911

Depositor

Stephen Elledge

Article

Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

Insert

DIF1 (DIF1 Budding Yeast)

Expression

Yeast

Mutation

HUG domain mutated VGMRIRQ->AAAAAAA, S59, Q66H

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+8NmCherry-ABCB4a

Plasmid

#34941

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCB4a

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Promoter

SP6

Available Since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+8NCerulean-ABCB4a

Plasmid

#34945

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCB4a

Use

Sea urchin, zebrafish, xenopus

Tags

Cerulean

Expression

Mammalian

Promoter

SP6

Available Since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+8CCerulean-ABCG2a

Plasmid

#34946

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCG2a

Use

Sea urchin, zebrafish, xenopus

Tags

Cerulean

Expression

Mammalian

Promoter

SP6

Available Since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+NmCitrine-ABCB1a

Plasmid

#34956

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCB1a

Use

Sea urchin, zebrafish, xenopus

Tags

mCitrine

Expression

Mammalian

Promoter

SP6

Available Since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
pGL4 [luc2P/ATF4-RE/Hygro] Vector

Plasmid

#236816

Purpose

Express ATF4 response element in luciferase reporter assay

Depositor

Promega Corporation

Has Service

DNA

Insert

ATF4 (ATF4 Human)

Use

Luciferase

Mutation

None

Promoter

minP

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-CreERT2

Plasmid

#14797

Purpose

Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Cre-ERT2 fusion protein

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pBsaBM3

Plasmid

#85102

Purpose

P450 BM3 variant (A74G/C773S/C810S)

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

P450 BM3

Use

Cloning vector

Mutation

A74G/C773S/C810S

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCVII-E2Crimson

Plasmid

#162756

Purpose

Retroviral (MSCV) expression of E2-Crimson fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

E2-Crimson

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-ERT2CreERT2

Plasmid

#13777

Purpose

Mammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

ERT2-Cre-ERT2 fusion protein

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pCAG-Cre

Plasmid

#13775

Purpose

Mammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Cre

Use

Cre/Lox

Tags

Myc

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pLYS1-FLAG-MitoGFP-HA

Plasmid

#50057

Purpose

Expression of GFP targeted to mitochondria with Flag and HA tags

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

Mito-GFP

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PPRE X3-TK-luc

Plasmid

#1015

Purpose

PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

Depositor

Bruce Spiegelman

Article

Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

Insert

PPRE

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 3, 2005

Availability

Academic Institutions and Nonprofits only

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pJSK659

Plasmid

#205128

Purpose

Expression of GFP-SnxA in mammalian cells

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

SnxA

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-pSu9-HaloTag7-mGFP

Plasmid

#184905

Purpose

Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

Presequence of Fo-ATPase subunit 9

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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lentiGuide-mCherry

Plasmid

#217005

Purpose

lentiviral plasmid for gRNA cloning with selectable mCherry expression

Depositor

Deepta Bhattacharya

Article

D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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