165,075 results

1. pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry

   Plasmid

   #203652

   Purpose

   Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   Lentiviral

   Tags

   Sapphire-IRES-H2B-mCherry

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pER-mScarlet3_N1

   Plasmid

   #189772

   Purpose

   Construct for labelling endoplasmic reticulum in mammalian cells using ER targeted mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   ER-mScarlet3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1 SARS-CoV-2 N

   Plasmid

   #158079

   Purpose

   Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 nucleocapsid (N )
   

   Expression

   Mammalian

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-FLAG-MTOR-S2215Y

   Plasmid

   #69013

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S2215Y

   Promoter

   CMV

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Gal:mCherry-uTEV3

   Plasmid

   #135453

   Purpose

   Yeast expression of uTEV3 fused to mCherry

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   mCherry-uTEv3
   

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   S219V mutation improves stability , I138T/S153N/T…

   Promoter

   Gal

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CoV2-M-IRES-E

   Plasmid

   #177938

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
   

   Expression

   Mammalian

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG/MNase

   Plasmid

   #123461

   Purpose

   Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.

   Depositor

   Steven Henikoff

   Article

   Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1

   Insert

   Protein A-Protein G-Micrococcal Nuclease
   

   Tags

   6xHIS and HA

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LPC-flag-REST-WT

   Plasmid

   #41903

   Depositor

   Stephen Elledge

   Article

   Westbrook et al Nature. 2008 Mar 20;452(7185):370-4. doi

   Insert

   RE1-silencing transcription factor (REST Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   May 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BB3cK_pGAP_23*_pTEF_Cas9

   Plasmid

   #104909

   Purpose

   hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   June 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pB-mTSPAN4-mCherry

    Plasmid

    #210330

    Purpose

    Overexpression of mTSPAN4

    Depositor

    Li Yu

    Article

    Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

    Insert

    mTSPAN4 (Tspan4 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge