We narrowed to 13,112 results for: CRISPR-Cas9

1. pLenti-multi-CRISPR-sgCasp8_#2-puro

   Plasmid

   #231978

   Purpose

   Knockout mouse Casp8

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-multi-CRISPR-sgCasp8_#1-puro

   Plasmid

   #231977

   Purpose

   Knockout mouse Casp8

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVS1-NDi-CRISPRi (Gen1)

   Plasmid

   #73497

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB-P2A-mCherry
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAVS1-NDi-CRISPRi (Gen2)

   Plasmid

   #73498

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CRISPRv2-sgIl1r1_#2-hygro

   Plasmid

   #231990

   Purpose

   Knockout mouse Il1r1

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   sgRNA with Cas9 and hygromycin resistance (Il1r1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX459-CTNNA1(α-catenin CRISPR KO)

   Plasmid

   #229708

   Purpose

   Knockout of a-catenin in mammalian cells using CRISPR-Cas9 technology

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR_eGFP_497

   Plasmid

   #111824

   Purpose

   Knockout eGFP

   Depositor

   Steve Shih

   Article

   Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

   Insert

   gRNA targeting eGFP 477-497
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPR_eGFP_191

   Plasmid

   #111821

   Purpose

   Knockout eGFP

   Depositor

   Steve Shih

   Article

   Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

   Insert

   gRNA targeting eGFP 171-191
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPR_eGFP_369

   Plasmid

   #111823

   Purpose

   Knockout eGFP

   Depositor

   Steve Shih

   Article

   Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

   Insert

   gRNA targeting eGFP 349-369
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRISPR_eGFP_314

    Plasmid

    #111822

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 294-314
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPRi

    Plasmid

    #199808

    Purpose

    vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

    Depositor

    Steve Salipante

    Article

    Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

    Inserts

    dCas9
    sgRNA with GFP cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid

    #91249

    Purpose

    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPR_RAF1_94

    Plasmid

    #111826

    Purpose

    Knockout Raf1

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting RAF1 94-114 (RAF1 Human)
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Plas-CRISPR_reporter

    Plasmid

    #157658

    Purpose

    CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene

    Depositor

    Frank Albert

    Article

    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

    Insert

    dCAS9-VP64, mCherry, KanMX
    

    Use

    Insert storage (replicative in e. coli)

    Tags

    Expression

    Mutation

    N28D in mCherry- please see depositor comments be…

    Promoter

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPRa_all-in-one

    Plasmid

    #183695

    Purpose

    Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPR-cBEST

    Plasmid

    #125689

    Purpose

    C to T base editor for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

    Insert

    streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPR-mcBEST

    Plasmid

    #209416

    Purpose

    Plasmid for multiplexed cytosine base editing in streptomycetes

    Depositor

    Tilmann Weber

    Article

    Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2

    Inserts

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    csy4 from Pseudomonas aeruginosa PAO1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    base editing cassette: PtipA ; sgRNAs: PkasO* an…

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PL-CRISPR.EFS.tRFP-gIfngr1-1

    Plasmid

    #166479

    Purpose

    Expresses spCas9, tRFP and a gRNA targeting mouse Ifngr1

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    gRNA for mouse Ifngr1 (Ifngr1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. PL-CRISPR.EFS.tRFP-gIfngr1-2

    Plasmid

    #166480

    Purpose

    Expresses spCas9, tRFP and a gRNA targeting mouse Ifngr1

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    gRNA for mouse Ifngr1 (Ifngr1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPR-cBEST-v2-PermE

    Plasmid

    #209446

    Purpose

    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site

    Depositor

    Tilmann Weber

    Article

    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

    Insert

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; sgRNA expression from ermE* promoter

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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