We narrowed to 11,588 results for: EMB

Showing: 3661 - 3680 of 11588 results

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6xHis(TEV)-Drp1-StrepII

Plasmid

#174422

Purpose

Expresses human Drp1 Isoform 3 in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Tags

6xHis(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pMCB619

Plasmid

#171011

Purpose

CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

U6:sgRNA_Puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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His-GFP-Rim2

Plasmid

#170869

Purpose

Mammalian expression of His-GFP-Rim2.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Regulating synaptic membrane exocytosis protein 2 (Rim 2) (Rims2 Rat)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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SpyTag003-OspC

Plasmid

#159998

Purpose

Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

SpyTag003-OspC (20-209) C129S

Tags

His6-tag and Thrombin cleavage site

Expression

Bacterial

Mutation

C129S mutation improves expression level.

Promoter

T7

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pET15-His-∆NGlpG

Plasmid

#155131

Purpose

Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Tags

His

Expression

Bacterial

Mutation

insert codes for GlpG residues 87-276

Promoter

T7

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-RFP-RGG

Plasmid

#124934

Purpose

Expression of RGG-RFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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lentiGuide-RRBP1-1

Plasmid

#92156

Purpose

CRISPR guide RNA targeting human RRBP1

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 sgRNA-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-TIS11B CPM2

Plasmid

#121143

Purpose

Mammalian expression of mCherry tagged TIS11B CPM2 (charge pattern mutant 2)

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

ZFP36L1 (TIS11B) charge pattern mutant 2 (VEGFA Human)

Expression

Mammalian

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pQE80L TriCatcher-RGDSP

Plasmid

#112631

Purpose

Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcher

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

TriCatcher-RGDSP

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

central RGDSP integrin-binding sequence

Promoter

T5

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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MIS12-FRB-tagRFP

Plasmid

#114039

Purpose

Kinetochore localised FRB tagged with FLAG and tagRFP

Depositor

Geert Kops

Article

Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

Insert

MIS12 (MIS12 Human)

Tags

FRB-FLAG-tagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Halo-Erk2 donor

Plasmid

#113104

Purpose

Donor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

N terminal Erk2 donor region including homology arms and HaloTag

Use

Synthetic Biology

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pHis6-hDF

Plasmid

#72585

Purpose

human dysferlin full length tagged with His

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin (DYSF Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only
pHsp70B (GB0155)

Plasmid

#68172

Purpose

Provides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtHsp70B promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pPML1 (GB0045)

Plasmid

#68164

Purpose

Provides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtML1 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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TAPBPL pmRFP-N2 (721)

Plasmid

#62000

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

TAPBPL (TAPBPL Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pFAST-BAC-Cdc20wt (2193)

Plasmid

#39850

Depositor

Jonathon Pines

Article

Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

Insert

Cdc20 (CDC20 Human)

Use

Baculovirus expression

Tags

His

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
Desmocollin2_tail-GST

Plasmid

#36992

Depositor

Kathleen Green

Article

Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

Insert

Dsc2 (DSC2 Human)

Tags

GST

Expression

Bacterial

Mutation

Intracellular domain of Dsc2 (nt 2,603–3,158)

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only
pT7-7 asyn S87A

Plasmid

#36058

Depositor

Hilal Lashuel

Article

Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

Insert

alpha-synuclein S87A (SNCA Human)

Expression

Bacterial

Mutation

S87A

Promoter

T7

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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TAL2364

Plasmid

#36777

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HDAC1-TALEN_Left (Hdac1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
KIFC1

Plasmid

#25253

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

KIFC1:G361-C715 (KIFC1 Human)

Use

Baculovirus expression

Tags

His

Mutation

Contains amino acids G361-C715

Promoter

polyhedrin

Available Since

Aug. 13, 2010

Availability

Industry, Academic Institutions, and Nonprofits