We narrowed to 7,122 results for: GFP expression plasmids

1. FLAG-LoxP-GFP-LoxP-SNAP-TERT

   Plasmid

   #71391

   Purpose

   Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.

   Depositor

   Thomas Cech

   Article

   Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

   Insert

   TERT (TERT Human)
   

   Use

   Hr donor

   Tags

   FLAG-SNAP

   Promoter

   Endogenous TERT promotor

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV hSyn FLEx-OFF Kir2.1-2A-GFP

   Plasmid

   #161580

   Purpose

   Express Kir2.1 in cells not expressing Cre.

   Depositor

   Robert Malenka, Kevin Beier

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   Kir2.1-2A-GFP (Kcnj2 Mouse)
   

   Use

   AAV

   Mutation

   NA

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520)

   Plasmid

   #139990

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-VQR with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   VQR=D1135V/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)

   Plasmid

   #139993

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   xCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160)

   Plasmid

   #139991

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   VRER=D1135V/G1218R/R1335E/T1337R

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

   Plasmid

   #139995

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   HF1=N497A/R661A/Q695A/Q926A

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

   Plasmid

   #113039

   Purpose

   AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. NLS-nCas9-NLS-P2A-EGFP (pRZ70)

   Plasmid

   #123616

   Purpose

   CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   NLS-nCas9-NLS-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CMV

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. nCas9-UGI-NLS-P2A-EGFP (pJUL1001)

   Plasmid

   #123611

   Purpose

   CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   nCas9-UGI-NLS-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CAG

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. (282) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4,K852A)

    Plasmid

    #160741

    Purpose

    Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to a GFP nanobody

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    HA-nGFP-(EAAAK)4-OGT(4,K852A)
    

    Tags

    HA-Tag

    Expression

    Mammalian

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBrain-GFP-TACC3KDP∆678-681-shTACC3

    Plasmid

    #69115

    Purpose

    Dual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

    Inserts

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    

    Use

    RNAi

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of 678-681aa region. Silent mutations i…

    Promoter

    CMV

    Available Since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMV-GFP-pA U6 shRNA (Rat NPAS4 #1)

    Plasmid

    #233270

    Purpose

    To express GFP from a CMV promoter and to express and shRNA targeting Rat Npas4

    Depositor

    Glenn Schafe, Jonathan Ploski

    Article

    Ploski et al PLoS One. 2011;6(8):e23760. doi: 10.1371

    Insert

    GFP and shRNA targeting Rat NPAS4 (Npas4 Rat)
    

    Use

    AAV

    Tags

    GFP

    Available Since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CMV-GFP-pA U6 shRNA (Rat NPAS4 #5)

    Plasmid

    #233271

    Purpose

    To express GFP from a CMV promoter and to express and shRNA targeting Rat Npas4

    Depositor

    Glenn Schafe, Jonathan Ploski

    Article

    Ploski et al PLoS One. 2011;6(8):e23760. doi: 10.1371

    Insert

    GFP and shRNA targeting Rat NPAS4 (Npas4 Rat)
    

    Use

    AAV

    Tags

    GFP

    Available Since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-cFOS-FRB-VP16-P2A-EGFP

    Plasmid

    #210510

    Purpose

    expresses cFOS-FRB-VP16 and EGFP component in rat hippocampal neuron cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    FRB-VP16-P2A-EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    cFos

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-N1 Dynamin1 T78R L84R T92R V118R

    Plasmid

    #112106

    Purpose

    Mammalian expression plasmid of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T78R L84R T92R V118R

    Promoter

    CMV

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1α1.1-ChrimsonR-GFP

    Plasmid

    #122063

    Purpose

    AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV2

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    EF1a（1.1kb short version)

    Available Since

    March 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. retro-gfp-puro vector

    Plasmid

    #58249

    Purpose

    Retroviral expression vector encoding bicistronic EGFP and Puromycin cassette

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Insert

    EGFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

    Plasmid

    #83925

    Purpose

    Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    eGFP
    Bsr
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A

    Promoter

    hUbC

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE

    Plasmid

    #102986

    Purpose

    AAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    membrane-targeted SNAP protein after FLP-mediated recombination
    Nuclear-targeted eGFP after FLP-mediated recombination
    

    Use

    AAV; Adeno associated viral vector

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Vsx2 R0+R1+R3 +SV40 base prom-GFP-IRES-AP

    Plasmid

    #225892

    Purpose

    Enhancer reporter

    Depositor

    Michael Dyer

    Article

    Honnell et al Development. 2024 Jul 1;151(13):dev20243

    Insert

    R0+R1+R3
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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