We narrowed to 18,451 results for: Met

1. spa-GCaMP6m

   Plasmid

   #67557

   Purpose

   photoactivatable fluorescent calcium reporter

   Depositor

   Ehud Isacoff

   Article

   Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

   Insert

   spa-GCaMP6m
   

   Tags

   HIS-tag

   Expression

   Mammalian

   Mutation

   K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pTYB3-human PUM1-HD PUF531_Q867E-Q939E-C935S-Q1011E- C1007S-intein-CBD

   Plasmid

   #73306

   Purpose

   Expresses mutant human PUM1-HD

   Depositor

   Traci Hall

   Article

   Wang et al Nat Methods. 2009 Nov;6(11):825-30. doi:

   Insert

   human PUM1 Pumilio homology domain (PUM1 Human)
   

   Tags

   intein

   Expression

   Bacterial

   Mutation

   Q867E-Q939E-C935S-Q1011E-C1007S

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

3. sspa-GCaMP6m

   Plasmid

   #67560

   Purpose

   short photoactivatable fluorescent calcium reporter

   Depositor

   Ehud Isacoff

   Article

   Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

   Insert

   sspa-GCaMP6m
   

   Expression

   Mammalian

   Mutation

   K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

4. TALEN-CCR5L18-NtermN2

   Plasmid

   #51446

   Purpose

   Expresses TALEN targeting left CCR5A site (L18) with N2 N-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Left (L18) N1 N-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 EL variant

   Expression

   Mammalian

   Mutation

   K150Q and K153Q

   Promoter

   CMV

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. TALEN-CCR5R18-NtermN2

   Plasmid

   #51447

   Purpose

   Expresses TALEN targeting right CCR5A site (R18) with N2 N-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Right (R18) N2 N-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 KK variant

   Expression

   Mammalian

   Mutation

   K150Q and K153Q

   Promoter

   CMV

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFAB3906

   Plasmid

   #47809

   Purpose

   BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD2 efficiency.

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   promoter 14 and BCD2
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   see insert

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSE89: pFuGW-G5p-EYFP

   Plasmid

   #240461

   Purpose

   Synthetic GAL4p comprising 5 tandem GAL4 binding sites (G5p) regulating EYFP

   Depositor

   Lior Nissim

   Article

   Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

   Inserts

   G5p
   EYFP
   

   Use

   Lentiviral

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSE92: pFuGW-G14p-EYFP

   Plasmid

   #240462

   Purpose

   A synthetic GAL4 promoter comprising 14 tandem GAL4 binding sites (G14p) regulating EYFP

   Depositor

   Lior Nissim

   Article

   Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

   Inserts

   EYFP
   G14p
   

   Use

   Lentiviral

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSE152: pFuGW-hUbCp-3CLpro-SV40NLS

   Plasmid

   #240455

   Purpose

   SARS-CoV-2 Protease 3CLpro expression plasmid

   Depositor

   Lior Nissim

   Article

   Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

   Insert

   3CLpro
   

   Use

   Lentiviral

   Promoter

   hUbCp

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. POC1550

    Plasmid

    #226497

    Purpose

    pMOBC360_VM_C1 assembled plasmid (Chloramphenicol R) containing four inserts (POC1546-POC1549) assembled in POC1525 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    

    Expression

    Bacterial

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. POC1546

    Plasmid

    #226487

    Purpose

    pMOBK360_VL_N1 assembled plasmid (Kanamycin R) containing four inserts (POC1535-POC1538) assembled in POC1518 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    ssembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    

    Expression

    Bacterial

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. POC1547

    Plasmid

    #226489

    Purpose

    pMOBK360_VM_N2 assembled plasmid (Kanamycin R) containing three inserts (POC1539-POC1541) assembled in POC1519 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    

    Expression

    Bacterial

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. POC1548

    Plasmid

    #226490

    Purpose

    pMOBK360_VM_N3 assembled plasmid (Kanamycin R) containing two inserts (POC1542-POC1543) assembled in POC1519 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    

    Expression

    Bacterial

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. POC1549

    Plasmid

    #226491

    Purpose

    pMOBK360_VR_N4 assembled plasmid (Kanamycin R) containing two inserts (POC1544-POC1545) assembled in POC1520 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    

    Expression

    Bacterial

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. POC1431

    Plasmid

    #221578

    Purpose

    Assembled plasmid containing four inserts (from POC1426, POC1427, POC1428 and POC1429) assembled using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    Assembled construct flanked by methylation-protectable BsaI sites and containing four always-methylable internal BsaI sites
    

    Use

    Synthetic Biology

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTol1-EcoRI-BmtI-BamHI-cmlc2-nmKate

    Plasmid

    #238372

    Purpose

    Empty backbone to generate guideRNA shuttles, that also drives expression of nuclear mKate in cardiomyocytes.

    Depositor

    Juan Manuel González-Rosa

    Article

    Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

    Insert

    nmKate
    

    Promoter

    cmlc2 (myl7)

    Available Since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTol1-Spe-KpnI-ubb-nmKate

    Plasmid

    #238373

    Purpose

    Empty backbone to generate guideRNA shuttles, that also drives ubiquitous expression of nuclear mKate.

    Depositor

    Juan Manuel González-Rosa

    Article

    Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

    Insert

    nmKate
    

    Promoter

    ubiquitin

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-hSyn-IvfChr-citrine-KV2.1

    Plasmid

    #221615

    Purpose

    A recombinant AAV2 plasmid encoding vfChrimson-citrine with trafficking enhancement and soma targeting with KV2.1 motif.

    Depositor

    John Lin

    Article

    Mermet-Joret et al eLife 2023 12:RP90327

    Insert

    vfChrimson-citrine with membrane trafficking enhancement and soma targeting
    

    Use

    AAV

    Mutation

    vfChrimson-citrine with membrane trafficking enha…

    Promoter

    Human Synapsin

    Available Since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRetro-UMI

    Plasmid

    #222686

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Mutation

    PGK without BbsI cutsite

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEN-X-mPGK-NeoR-SV40 polyA-Z

    Plasmid

    #218909

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    mPGK-Neomycine-SV40 polyA
    

    Expression

    Mammalian

    Mutation

    395T>C

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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