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iRFP-PAmCherry1-PH_PLCd1

Plasmid

#92167

Purpose

PI(4,5)P2 single molecule fluorescent reporter

Depositor

Xuelin Lou

Article

Ji et al J Biol Chem. 2015 Nov 6;290(45):26978-93

Insert

iRFP-PAmCherry1-PH_PLCd1

Tags

iRFP-PAmCherry1

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC-mTRF1

Plasmid

#64162

Purpose

Retroviral vector expressing mouse TRF1 with N-terminal Myc tag

Depositor

Titia de Lange

Article

Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

Insert

mTRF1 (Terf1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only
pET28C-mCherry-HNRNPA1LCD

Plasmid

#204405

Purpose

E. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

HNRNPA1 (186-320) (HNRNPA1 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Mito-LSSmGFP

Plasmid

#186536

Purpose

Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to mitochondria

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Tags

Human COX8a first 29 amino acids

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pTalpha1-Dre

Plasmid

#133925

Purpose

Neuron-specific expression of Dre

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

HA-Dre

Tags

HA

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pNIC-AncCAR_A

Plasmid

#168286

Purpose

Expresses AncCAR_A in E. coli from a T7 promoter

Depositor

Nicholas Harmer

Article

Thomas et al Commun Biol. 2019 Nov 22;2:429. doi: 10.

Insert

AncCAR_A

Use

Synthetic Biology

Tags

6*His

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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TagRFP-T-VAPB(1- 218)- eMagB

Plasmid

#162255

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

TagRFP-T-VAPB(1- 218)- eMagB

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pXWS40tetO

Plasmid

#160827

Purpose

40 repeats of tetO sponge

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

(40 X tetO)-t

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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DmTmem63

Plasmid

#136598

Purpose

Expresses the mechanosensitive channel Drosophila melanogaster Tmem63 in mammalian cells

Depositor

Ardem Patapoutian

Article

Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

Insert

DmTMEM63

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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OSCA3.1

Plasmid

#136596

Purpose

Expresses the mechanosensitive channel OSCA3.1 in mammalian cells

Depositor

Ardem Patapoutian

Article

Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

Insert

OSCA3.1 (ERD4 Mustard Weed)

Tags

pIRES2-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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CLXSN 5myc-CDC6-mut

Plasmid

#109333

Purpose

express tagged Cdc6 mutant

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

CDC6 (CDC6 Human)

Use

Retroviral

Tags

5x Myc

Expression

Mammalian

Mutation

R56A, L59A, K81A, E82A, N83A

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pUA66 PcspA GFP No Linker

Plasmid

#105605

Purpose

Expresses GFP under temperature sensitive promoter PcspA

Depositor

Pamela Silver

Article

Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

Insert

GFP

Expression

Bacterial

Promoter

PcspA

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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p3s-Cas9-HF1

Plasmid

#104173

Purpose

Cas9-HF1 mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

Cas9-HF1

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLC242-GFP-metap2

Plasmid

#100518

Purpose

Expresses GFP tagged metap2 in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

Metap2 (METAP2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SAMTOR (D190A)

Plasmid

#100515

Purpose

Expresses HA tagged SAMTOR D190A mutant in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Tags

HA

Expression

Mammalian

Mutation

D190A

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRAF-MutS

Plasmid

#86605

Purpose

E. coli MutS expression plasmid

Depositor

Richard Fishel

Article

Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

Insert

E. coli MutS (D835R,R840E)

Tags

his-tag and ald-tag

Expression

Bacterial

Mutation

D835R, R840E

Promoter

T7

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-Cdi

Plasmid

#83044

Purpose

Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)

Depositor

Jennifer Doudna

Article

Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

Insert

CdiCas9

Tags

His-MBP

Expression

Bacterial

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only
Sox1/2/3-2373/+3>nls::Cas9::nls

Plasmid

#59989

Purpose

Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Sox1/2/3 (SoxB1) -2373/+3

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pGLB2 5'3'

Plasmid

#49346

Purpose

luciferase reporter with Bmp2 promoter (1702 bp) and Bmp2 3'UTR (2015 bp)

Depositor

Melissa Rogers

Article

Fritz et al J Biol Chem. 2004 Nov 19;279(47):48950-8

Inserts

Bmp2 distal promoter (Bmp2 Mouse)
Bmp2 3' UTR (Bmp2 Mouse)

Use

Luciferase

Tags

luciferase

Promoter

-

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B8-Leum

Plasmid

#44316

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-Leum

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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