We narrowed to 9,628 results for: otos

1. pYFAC-CH1

   Plasmid

   #168977

   Purpose

   AMA1 based expression in filamentous fungi. pYFAC-pyrG carrying PalcA ; unique restriction site: PacI

   Depositor

   Yit Heng Chooi

   Article

   Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

   Type

   Empty backbone

   Use

   Synthetic Biology; Heterologous expression in fil…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEG BacMam C term 3C eGFP myc

   Plasmid

   #160685

   Purpose

   Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Use

   Tags

   His8 eGFP 3C

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFIN-XOPS-tdTOM

   Plasmid

   #44359

   Depositor

   Susan Semple-Rowland

   Article

   Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

   Insert

   XOPS-tdTOM
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Xenopus Opsin (XOPS) (pos 3125-4501)

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-TRE-Tight2-AkaLuc

   Plasmid

   #186186

   Purpose

   AAV vector packaging AkaLuciferase under the TRE promoter

   Depositor

   Vladislav Verkhusha

   Article

   Kasatkina et al Nat Commun. 2022 May 19;13(1):2813. doi:

   Insert

   AkaLuc luciferase
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   TRE-Tight2

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYFAC

   Plasmid

   #168982

   Purpose

   Filamentous fungi vector. Also referred to as pYFAC-pyrG.

   Depositor

   Yit Heng Chooi

   Article

   Chooi et al Environ Microbiol. 2017 May;19(5):1975-1

   Type

   Empty backbone

   Use

   Synthetic Biology; Heterologous expression in fil…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mOrange-H2B-6

   Plasmid

   #57942

   Purpose

   Localization: Nucleus/Histones, Excitation: 548, Emission: 562

   Depositor

   Michael Davidson

   Article

   Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   mOrange

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available since

   Oct. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

7. BcLOV-mCh-SOS

   Plasmid

   #208279

   Purpose

   Expresses BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

   Depositor

   Lukasz Bugaj

   Article

   Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

   Insert

   BcLOV-mCh-SOS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Yellow Cameleon-Nano30/pcDNA3

   Plasmid

   #51963

   Purpose

   mammalian expression of Yellow Cameleon-Nano30, an ultrasensitive Ca2+ indicator with Kd = 30 nM

   Depositor

   Takeharu Nagai

   Article

   Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

   Insert

   Yellow Cameleon-Nano30
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pGFP-PTEN G129E

   Plasmid

   #50523

   Depositor

   Kenneth Yamada

   Article

   Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   G129E

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

10. mOrange2-H2B-6

    Plasmid

    #57962

    Purpose

    Localization: Nucleus/Histones, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

11. MAC-SARS-CoV-2 Orf9c

    Plasmid

    #158379

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 Orf9c
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCAG-Gdf9

    Plasmid

    #163599

    Purpose

    Expresses GDF-9 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Gdf9 (Gdf9 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS104 pHAGE2 CMVtetO2 MCS-3C-22xGS-GFP

    Plasmid

    #199357

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV 4xSTAR-mTFP-NLS-blast

    Plasmid

    #136257

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    mTFP-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3 CrkII

    Plasmid

    #50731

    Purpose

    mammalian expression of CrkII

    Depositor

    Kenneth Yamada

    Article

    Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

    Insert

    CrkII (CRK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCfB8792

    Plasmid

    #126910

    Purpose

    Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    ADE2 gRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3

    Plasmid

    #21800

    Depositor

    David Ron

    Article

    Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

    Insert

    PSMD14 (Psmd14 Mouse)
    

    Use

    Tags

    GST and TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJL017

    Plasmid

    #53532

    Purpose

    This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

    Depositor

    James Fleckenstein

    Article

    Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

    Insert

    etpBA
    

    Use

    Tags

    6His and myc

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. MPC020: CamKII CMV_NovArch_citrine

    Plasmid

    #153193

    Purpose

    Encodes Novarch

    Depositor

    Adam Cohen

    Article

    Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

    Insert

    NovArch
    

    Use

    Tags

    citrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT2A-EKAREV-NLS

    Plasmid

    #173856

    Purpose

    Encoding EKAREV-NLS.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    EKAREV-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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