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RalGDS-YPet
Plasmid#134929PurposeFluorescently tagged affinity reagentDepositorInsertRaIGDS RBD, YPet
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK5 K232R
Plasmid#80878Purposemammalian expression of ALK5 K232RDepositorInsertALK5 (TGFBR1 Human)
UseTagsHAExpressionMammalianMutationK232R (kinase dead)PromoterCMVAvailable sinceAug. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Orange Nano-lantern(Ca2+)/pcDNA3
Plasmid#71346PurposeExpress Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cellDepositorInsertOrange Nano-lantern(Ca2+)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
Plasmid#159803PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p8103 LentiCRISPR v2 sgNT-2
Plasmid#163313PurposeExpresses Cas9 and a non-targeting control guide RNADepositorInsertsgNT-2
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHis2-Arf1 (17-181)-Q71L
Plasmid#184808PurposeBacterial expression of Arf1 (aa17-181, Q71L mutation)DepositorInsertArf1 (ARF1 Human)
UseTagsHis6ExpressionBacterialMutationaa 17-181 only, Q71LPromoterT7Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJAB988
Plasmid#133920PurposeminiICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoterDepositorInsertminiICE-tet(M)cat(PC194)_PT7-GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-RIPK3
Plasmid#78822PurposeTo detect localization of RIPK3 protein in live mammalian cellsDepositorInsertRIPK3 (RIPK3 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV103
Plasmid#63193PurposepRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionWorm and YeastMutationPromoterAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUChW Parkin shRNA Mul1 shRNA
Plasmid#78523PurposeLentiviral vector encoding two shRNAs and mCherry reporterDepositorUseLentiviral and RNAiTagsExpressionMutationPromoterH1Available sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV_nLightR
Plasmid#217657PurposeExpresses norepinephrine indicator nLightR under mammalian promoterDepositorInsertnLightR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
EK0307 CMV-TO-BFPmut-stop-3UTR(Bdnf) (FLP-IN)
Plasmid#191145PurposeExpression of a synthetic construct with 3' UTR of murine Bdnf in the 3' UTR of an inactive BFP in mammalian cells.DepositorInsert3UTR(Bdnf)
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-nCas9(D10A)-PmCDA1
Plasmid#79617PurposeExpresses nCas9(D10A)-PmCDA1 in yeast cellsDepositorInsertSpCas9
UseTagsPmCDA1ExpressionYeastMutationD10A for nickase Cas9PromoterpGal1Available sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spCas9-ABE-Umax-rest1
Plasmid#222144PurposeAdenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9
UseCRISPR; Zebrafish expressionTagsBipartite NLSExpressionMutationPromoterT3Available sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-AviTag-DogCatcher-MBP
Plasmid#171928PurposeExpresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.DepositorInsertAviTag-DogCatcher-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationDogCatcher added to N-terminus of MBP via a GSGES…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-6011nes
Plasmid#108652PurposeAn ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-ERK-EV
UseTagsXFPExpressionMammalianMutationPromoterpCAGGSAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMK365 (Tet-OsTIR1-loxP-PURO-loxP)
Plasmid#121185PurposeOsTIR1 introductionDepositorInsertTet-OsTIR1-loxP-PURO-loxP
UseCRISPRTagsExpressionMutationOsTIR1 codon optimized for human cellsPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro LAP2
Plasmid#15712DepositorInsertLAP2 (CEBPB Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDV02_linear_entry_TagBFP-2A-MCS-2A-mNeonGreen
Plasmid#200401PurposeEntry vector for open-loop ADAR-based RNA sensors. Expresses TagBFP constitutively. Expresses mNeonGreen upon target detectionDepositorInsertOpen-loop DART VADAR sensor entry cassette
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only
