We narrowed to 26,489 results for: CAL;

1. pHH0103_CAPNS1_EF-hand_8+EF-hand_5

   Plasmid

   #109995

   Purpose

   Protein expression and purification of CAPNS1_EF-hand_8+EF-hand_5

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   CAPNS1_EF-hand_8+EF-hand_5 (CAPNS1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. SspB-mCherry-DHPH-TIAM1

   Plasmid

   #176104

   Purpose

   Heterodimerization with iLID, Rac1 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   TIAM1 (TIAM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2+-HypocratesCS

   Plasmid

   #161960

   Purpose

   Mammalian expression of non-targeted inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives

   Depositor

   Dmitry Bilan

   Article

   Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:

   Insert

   Hypocrates
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Cysteine 355 to Serine

   Promoter

   CMV, SP6

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

   Plasmid

   #199252

   Purpose

   Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   High specificity eSpCas9(1.1)-D10A nickase
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A K848A K1003A R1060A

   Promoter

   CAG promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR

   Plasmid

   #67275

   Purpose

   For sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell lines

   Depositor

   Jannik Elverløv-Jakobsen

   Article

   Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do

   Insert

   puromycin resistant gene
   

   Use

   Tags

   linked to red flouresent protein gene (katushka)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Rat Alpha2 delta-1 HA pMT2

   Plasmid

   #58729

   Purpose

   expression of HA tagged rat Alpha2 delta-1 in mamalian cells

   Depositor

   Annette Dolphin

   Article

   Kadurin et al J Biol Chem. 2012 Sep 28;287(40):33554-6

   Insert

   alpha2 delta-1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGH

   Plasmid

   #74289

   Purpose

   pAAV vector to express H2B-GFP and oG (optimized Glycoprotein) in a Cre-dependent manner

   Depositor

   Edward Callaway

   Article

   Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

   Insert

   H2B-GFP and oG (optimized Glycoprotein)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   chimeric glycoprotein

   Promoter

   Ef1a

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1a-GCaMP6s-WPRE-pGHpA

   Plasmid

   #67526

   Purpose

   "Fluorescent reporter for calcium imaging"

   Depositor

   Botond Roska

   Article

   Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   6xHis, T7 tag, and Xpress epitope

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTU422

   Plasmid

   #178982

   Purpose

   13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)

   Depositor

   Stan Brouns

   Article

   van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

   Inserts

   gRAMP
   CRISPR array
   

   Use

   Tags

   Strep-Tag II SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. SspB-HaloTag-DHPH-TIAM1

    Plasmid

    #176114

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLL7.0: mTiam1(64-437)-tgRFPt-SSPB R73Q

    Plasmid

    #60418

    Purpose

    Mammalian Expression of Tiam DH/PH-tgRFPt-Micro

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    mTiam1-tgRFPt-SSPB R73Q
    

    Use

    Cre/Lox and Lentiviral

    Tags

    tgRFPt

    Expression

    Mammalian

    Mutation

    mTiam 64-437 // SSPB R73Q, Y11K and A15E

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. DRH335: FCK-Optopatch1

    Plasmid

    #51695

    Purpose

    Coexpression plasmid of QuasAr1 and CheRiff for all-optical electrophysiology

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    Optopatch1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. HT110_FCMV_QuasAr6b_citrine

    Plasmid

    #178823

    Purpose

    Expresses an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag

    Depositor

    Adam Cohen

    Article

    Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

    Insert

    QuasAr6b_citrine
    

    Use

    Lentiviral

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40

    Plasmid

    #100855

    Purpose

    AAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    jRGECO1b
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDule-3-nitroTyrosine (5B)

    Plasmid

    #85498

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32H H70C D158S I159A L162R

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. SspB-iRFP-DHPH-TIAM1

    Plasmid

    #176118

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTol2-CreLite

    Plasmid

    #131783

    Purpose

    Tol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PhyBCreC, PIF6CreN, and mTagBFP2
    

    Use

    Cre/Lox and Synthetic Biology; Zebrafish tol2 tra…

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Ubi

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-ASAP2s

    Plasmid

    #101276

    Purpose

    Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

    Depositor

    Francois St-Pierre

    Article

    Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

    Insert

    ASAP2s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn (human synapsin)

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.hSyn1.Twitch2B.WPRE.SV40

    Plasmid

    #100040

    Purpose

    AAV expression of Twitch2B driven from hSyn1 promoter. Twitch 2B is a fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Has Service

    AAV1

    Insert

    Twitch2B
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. dmBACCS2NS-IRES-dOrai-IRES-GFP

    Plasmid

    #72895

    Purpose

    Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2NS-IRES-dOrai-IRES-GFP
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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